Báo cáo khoa học: Phosphorylation modulates the local conformation and self-aggregation ability of a peptide from the fourth tau microtubule-binding repeat pdf
... or SYK themselves, is capable
of transforming NIH-3T3 cells [70]. In addition, we
and others have demonstrated that the activation and
plasma membrane localization of the fusion construct
are dependent ... key
tyrosines that are subject to auto- and ⁄ or transphosph-
orylation, and that mediate interaction with Vav,
c-Cbl and the p8 5a subunit of phosphatidylinositol
3-...
... centrifugation through an oil
cushion and dissolved prior to determination of the
amount of dyes associated with them. The advantages
of such an assay are that: (a) it is quantitative; (b) it is
not affected ... they are
hydrophobic and negatively charged. A comparison of
rhodamine uptake, in the presence and the absence
of uncouplers, allows the estimation of t...
... significant advantage that enthalpy
changes can be monitored directly and data obtained
in a model-free fashion [36,37]. Disadvantages of calo-
rimetry are the high concentrations and amounts of
material ... reports
documenting that the lengths of the pre- and post-
transition baselines selected and used in data analysis
directly affect the values of the thermodynam...
... can put as many features as
possible together under the log-linear model to eval-
uate the quality of a phrase and a phase pair. The
phrase table extracting procedure is trainable and
can be optimized ... used to evaluate the quality of a
phrase and a phrase pair. Ideally, a perfect combina-
tion of feature functions divides the correct and in-
correct candid...
... exponential phase, and then incubated for 30 min in galac-
tose medium to activate the GAL regulon. Total mRNAs were
extracted and subjected to standard northern blot analysis. GAL1
mRNA, and ACT1 ... the
S13 4A- S ⁄ S13 4A- AS, S13 3A- S ⁄ S13 3A- AS, S13 3A- S13 4A-
S ⁄ S13 3A- S13 4A- AS and S134D-S ⁄ S134D-AS pairs of
primers (Table S2). Transformation of yeast cells with...
... -ATC ATC TCC
ATC GAC TAC TCC CTG-3¢, the antisense primer
5¢-AAG AAT
TCT AGA TTA ATG GTG ATG ATG GTG
ATG ATG GTG TGG GGT CAG CGG TGC AGC AGG
GGG GGT-3¢ (XbaI sites underlined; His-tag in italic) ... by bis-ANS was
decreased upon phosphorylation. A possible explana-
tion for this apparent discrepancy could be that PKA
phosphorylation induces a conformational change that
increases the...
... 3¢-GG
AACCACGAAAGTAG CAATC AGA ACACTA AAACC A
GGTACAATGATGC-5 ¢ . All vector inserts were
sequenced prior to use.
CDK4 pY17 antibody generation
Murex lop-eared rabbits were injected with a phosphopep-
tide ... 537 of
C-YES was mutated from TAC (Tyr) to TTC (Phe) with
the forward primer: 3¢-GTCACAGAGCCACAGTTCCAG
CCAGGA-5¢. Codon 305 of C-YES was mutated from
AAA (Lys) to AGA (Arg...
... 5¢-TCATGCTGGTTGAGACTG
GA-3¢ and 5¢-CCAGGTGTGGAGCAAGGCAG-3¢) and a
938 bp fragment of promoter 3 (primers, 5¢-CTCACAG
CAGCCAGTAAGTG-3¢ and 5¢-ACTCACAGGCTCTGG
GGCTG-3¢) were amplified using a Taq polymerase ... 5¢-GGACTCAAGAGAGGAACCTG-3¢; F1b, 5¢-CT
GCCTTGCTCCACACCTG-3¢; F1c, 5¢-CTGCCGTCTCC
GCCGCCACT-3¢; FU, 5¢-TCAAGCCCCGCTACATAG
TT-3¢; and R3, 5¢-AGGAACCAAACACCAAGTGG-3¢
(Fig. 2A)...
... from cleavage of the core residue Gal
(Fig. 3A) , and by
0,3
A
5
at m/z 1111. The C
2a
ion at m/z 528
shows that one Fuc is at the subterminal GlcNAc, and the
characteristic D
1a- 2a
ion [13] at ... fragment ions from both branches, and as
no additional fragment ions are apparent, it can be deduced
that the disaccharide sequence on the 3-branch shares the
same...
... both
iab-5 and iab-6 are active, and iab-7 and iab-8 are
silenced, but only iab-6 drives the expression of Abd-B.
Similarly, although three different Abd-B cis-regulatory
domains are active in A7 , the ... of these sequences are derived from the
Mcp [11], and the Fab-7 [12] regions. Both contain a
boundary and at least one PRE, and are able to medi-
ate long-dista...