... Y, Singh G, Yamanaka H, Tanaka E, Urano
W, Taniguchi A, Saito T, Hara M, Tomatsu T &
Kamatani N (2003) Validation of a Japanese version of
the Stanford Health Assessment Questionnaire in 3,763
patients ... 3,763
patients with rheumatoid arthritis. Arthritis Rheum 49,
784–788.
21 Ikari K, Kuwahara M, Nakamura T, Momohara S, Hara
M, Yamanaka H, Tomatsu T & Kamatani N (2005...
... control was maintained in a standard incubator with 21% O
2
. The normoxia data are the same as shown in
Fig. 1 and are included here for comparison. Expression of miR-2 7a and miR-27b at the indicated ... treated as described in (A) . Total RNA was
prepared at the indicated times and subjected to quantitative real-time PCR analysis. The data shown are mean value ± standard errors...
... product was 1 : 10 diluted
and used as a template in an additional PCR, using attB
primers EVD 2 (5¢-GGGGACAAGTTTGTACAAAAA
AGCAGGCT-3¢) and EVD 4 (5¢-GGGGACCACTTTG
TACAAGAAAGCTGGGT-3¢) in order ... (Galanthus nivalis
agglutinin, GNA) in P. pastoris. A glucose-mannose-
binding legume lectin from the seeds of Canavalia
brasiliensis, a homolog of the classical vacuolar conca-
n...
... The role of the ESSS protein in the assembly of a functional and stable
mammalian mitochondrial complex I (NADH-ubiquinone
oxidoreductase)
Prasanth Potluri, Nagendra Yadava and Immo E. ... notable insights.
Finding mutations in mammalian systems affecting
complex I has been even more o f a challenge. A systematic
investigation of human patients suffer ing from mitochond-
r...
... indicate that the intersubunit disulfide bonds
of BS-RNase have a key role in the mechanism of
antitumour action of the enzyme.
Materials and methods
Materials
Iodoacetic acid (IAA), iodoacetamide ... Poly-
clonal antibodies against BS-RNase, obtained from rabbits
as described previously [11], were used at a dilution of
1 : 1000. Fluorescein-tagged goat anti-rabbit secondary...
... serine
proteases do not have an aspartate as the catalytic apparatus.
However, for chymotrypsin-type serine proteases, the
replacement of this aspartate with an alanine diminishes
protease activity ... Osaka, Japan;
3
School of Knowledge Science, Japan Advanced Institute of Science and Technology,
Ishikawa, Japan
Achromobacter protease I (API) has a unique region of
aromatic ring st...
... software such as aggrescan [42] or tango [43].
Protein aggregation can be understood as an anoma-
lous type of protein–protein interaction. As for native
interactions, the attainment of ordered aggregated
structures ... Barcelona, Spain
3 CIBER de Bioingenierı
´
a, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain
4 Department of Biochemistry and Molecular Biology, Universitat...
... was accompanied by the appearance, from about
day 12, of May–Gru
¨
nwald ⁄ Giemsa-positive granular
structures in SG
+ ⁄ +
cells. A gradual increase in
May–Gru
¨
nwald ⁄ Giemsa staining was seen ... an alternative degradative pathway in the
absence of SG. However, incubation of cells with lac-
tacystin, an inhibitor of proteasome function, did not
cause any accumulation of...
... Meyer-Klaucke for data collection and assis-
tance in data evaluation. The assistance of T. Pavkov
(Institute of Chemistry, University of Graz) in the
acquisition of CD and DLS data is gratefully acknowl-
edged. ... metal-binding recognition
as well as reactivity in chemical transformations. Func-
tional annotation of cupin proteins from sequence and
3D structural data alone...
... proteins involved in
signal transduction and vesicle trafficking, specifically
associate to negatively charged membranes.
As all these data are suggestive of a role of mem-
branes, in particular of ... proteins with cytotoxic activ-
ity: BS-RNase [10] is a natural dimeric RNase; RNase-
AA [21], RNase-AA-G [21], and RNase-AA-GG [22]
are dimeric variants engineered from bovine p...