... 9, 10 and 13 ), whereas deletion of 296
residues (VP16-DEC1:4 11 6) decreas ed the binding (lane
11 ), and deletion of 312 residues (VP16-DEC1:4 10 0)
abolished it (lane 12 ). Expression levels of ... 273 residues from the C-terminal region
of DEC1 (DEC1 :1 309, DEC1 :1 232 and DEC1 :1 13 9)
had little effect on the suppressive activity of DEC1.
Expression of these trunc...
... with FnBRAD9 ,10 as compared with the binding
of 15 E 11 to the intact repeat region (Fig. 6C,D). The
residual binding of 15 E 11 to FnBRAD9 ,10 could be a
consequence of the weak binding of repeats ... (MI:0049)
l
MINT-79 911 03, MINT-79 911 14, MINT-79 911 26, MINT-79 911 38, MINT-79 911 53, MINT-
79 911 65, MINT-79 911 77: fnbB (uniprotkb:Q53682) binds (MI:0407)toFibron...
... involved the insertion
of several fragments of the 5¢-region of mce -1 into the mul-
tiple cloning site of the vector pPD95.77 provided by A.
Fire (Carnegie Institute, Baltimore, MD, USA). The inser-
ted ... AM1.
J Bacteriol 18 4, 17 50 17 58.
J. Ku
¨
hnl et al. Methylmalonyl-CoA epimerase from C. elegans
FEBS Journal 272 (2005) 14 65 14 77 ª 2005 FEBS 14 75
11 Pfeif...
... were spotted onto PEI
cellulose sheets with 4 lL of nonradioactive tracer (10 0 lg
of 5-FU and 10 0 lg of uridine for the UP assay mixture;
10 0 lg of 5-FU and 10 0 lg of deoxyuridine for the TP
assay ... homolog. (B) The expression
patterns of T07C4 .1: :GFP and
R12E2 .11 ::GFP are shown. T07C4 .1: :GFP
expression is robust in neurons and the
intestine, whereas R12E2 ....
... Effect of pyruvate kinase overpro-
duction on glucose metabolism of Lactococcus lactis.
Microbiology 15 0, 11 03 11 11.
18 Thomas S & Fell DA (19 98) A control analysis explora-
tion of the role of ... performed in strain CS1897
which contains an additional copy of pyk in
the TP9 011 -1 phage attachment site. A
double cross-over event of the resulting
plasmid, pCS...
... L. Bang et al. Functional analysis of GALE mutation in ldlD cells
FEBS Journal 276 (2009) 19 52 19 61 ª 2009 The Authors Journal compilation ª 2009 FEBS 19 61
Functional analysis of mutations in ... on the ratio
of intensity of the myc–GALE to tubulin bands in the western blot
and normalized by setting the amount of GALE in ldlD(myc–GALE)
wt #11 as 1. 0. The...
... absorbancies of the
two compounds (Fig. 3). The 52 kDa ETB
cHx
elutes at a
retention volume of 1. 6 mL, whereas the 21- mer cET -1
starts to elute at a volume of 2 .1 mL. Coelution of
cET -1 with ETB
cHx
therefore ... Different distribution of
endothelin receptor subtypes in pulmonary tissues
revealed by the novel selective ligands BQ -12 3 and
[Ala
1, 3 ,11 ,15
]ET -1...