... level of
Fig. 1. NGF induces expression of the
microRNAs miR-221 and 222 in PC12 cells.
(A) NGF- induced differentiation of PC12
cells. (B) Schematic representation of the
genomic structure of miR-221 ... Sustained activation of ERK1/2 by NGF induces
microRNA-221 and 222 in PC12 cells
Kazuya Terasawa
1
, Atsuhiko Ichimura
1
, Fumiaki Sato...
... indu ce expression of
genes involved in cell cycle progression include Elk1, c-fos,
c-Jun and c-myc [9,15]. The duration of ERK activation
(transient or sustained) determines the repertoire of ... cell su rface.
In addition it cau ses a decreased EGF bin ding affinity and tyrosine
kinase activity of the r eceptor. Also shown are the stimulators and
inhibitors(depictedinred)...
... blocked
inactivation of the AMPK by insulin (Fig. 7), suggesting a
dominance of the fatty acid-driven pathway for activation
of AMPK over at least some aspects of insulin signalling.
This dominance of ... the activation of AMPK by fatty acids
and insulin and adrenergic signalling processes
Fig. 5 shows studies focused on the dominant a-2 AMPK
isoform. Insulin decreased...
... using
crotonyl-CoA as a substrate [15]. The gene encoding
this bifunctional protein was named AUH (‘AU bind-
ing homolog of enoyl-CoA hydratase’). The RNA-
binding activity of the human protein ... portion of both fusion pro-
teins was removed by proteolysis and the resulting pro-
teins AUHp40 and AUHp30 were again purified by
chromatography. Thus, four different pure fractions...
... comparative immunoblotting data, it was of interest
to re-examine the fate of cals equestrin by studying the entire
complement of key Ca
2+
-binding elements in dystrophin-
deficient skeletal muscle ... for
silver staining, hot Coomassie s taining, ÔStains-AllÕ labelling
or immunoblotting. T he following running conditions were
used: 60 m in at 1 00 V , 6 0 m in at 500 V, 60 min...
... channeling
Prior investigations of channeling may be divided into
two categories: in vitro and in vivo. The former include
experiments with cell extracts and purified enzymes.
These are inherently unsatisfying. ... studies of channeling of
aminoacyl-tRNA for protein synthesis. The cells were
electroporated to facilitate entry of
3
H-labeled amino-
acyl-tRNA and
14
C-labeled...
... study of the role of recombination in
mtDNA inheritance in S. cerevisiae showed that budding
cells were enriched in linear monomers of mtDNA. It was
proposed that in addition to a role in initiating ... mobility binding assays with increasing concentrations
of control Ydc2 and Ydc2-ND35 (A) or Ydc2-ND35/CD15 (B), as
indicated, visualized by phosphorimaging. (C) Binding...
... invention” and the joining phrase was
by ; one of the queries would be:
“invention by student” OR “invention by a student” OR
“invention by the student”
This returns the sum of the number of pages ... conditions, and
again using all of the joining terms produced the
best results.
The SVM algorithm produced the best accuracy
of all, achieving 50.1% accuracy using t...
... Analysis of data was
performed with
ORIGIN
6.1 and
SIGMA PLOT
4.2 software. A
direct plot of activity vs. [GTP] gave in all cases a sigmoidal
curve indicating cooperative substrate binding. In order ... the amount of antigen and was used to
quantify the amounts of GCAPs. An example is shown in
Fig. 1A,B, where the amount of GCAP standards showed a
linear increase in chem...
... containing
the last 326 amino acids in the case of Pta-F1, the last 352
amino acids in the case of Pta-F2, and the last 470 amino
acids in the case of Pta-F3 (Fig. 2).
PCR reactions performed in ... FEBS
Results
Expression and purification of E. coli Pta and
truncated Ptas containing the C-terminal end
By analysis of the protein domain architecture of
E. coli Pta, t...