Báo cáo khoa học: Molecular basis of the unusual catalytic preference for GDP/GTP in Entamoeba histolytica 3-phosphoglycerate kinase doc

Báo cáo khoa học: Molecular basis of the unusual catalytic preference for GDP/GTP in Entamoeba histolytica 3-phosphoglycerate kinase doc

Báo cáo khoa học: Molecular basis of the unusual catalytic preference for GDP/GTP in Entamoeba histolytica 3-phosphoglycerate kinase doc

... structures of the wild-type, single- and double-mutant EhPGKs in a closed conformation will certainly help to clarify the molecular arrangement in the guanine ring binding site during the binding of ... carbon 2 of the guanine ring may interact with the side chain of Glu309, whereas the carbonyl group at position 6 of the guanine ring may interact with the hy...
Ngày tải lên : 16/03/2014, 01:20
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Báo cáo khoa học: Molecular basis of perinatal hypophosphatasia with tissue-nonspecific alkaline phosphatase bearing a conservative replacement of valine by alanine at position 406 Structural importance of the crown domain potx

Báo cáo khoa học: Molecular basis of perinatal hypophosphatasia with tissue-nonspecific alkaline phosphatase bearing a conservative replacement of valine by alanine at position 406 Structural importance of the crown domain potx

... soluble version of the mutant protein. The K cat ⁄ K m value of the mutant TNSALP was less than one-tenth of the K cat ⁄ K m value of the soluble form of the wild-type enzyme, indicating that the replacement ... resulting in a K cat ⁄ K m value that was < 10% of that of the wild- type enzyme (Table 1), indicating that the conversion of valine to alanine...
Ngày tải lên : 07/03/2014, 05:20
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Tài liệu Báo cáo khoa học: Molecular basis of glyphosate resistance – different approaches through protein engineering doc

Tài liệu Báo cáo khoa học: Molecular basis of glyphosate resistance – different approaches through protein engineering doc

... An in silico docking analysis of glyphosate binding at the GO active site showed that glyphosate is bound in the same orienta- tion as inferred for glycine (with the phosphonate moi- ety pointing ... in the binding energy for this small substrate. Because of the introduction of an arginine at position 54, the a2–a3 loop (comprising resi- dues 50–60) assumes a diffe...
Ngày tải lên : 14/02/2014, 14:20
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Báo cáo khoa học: Molecular mechanisms of the phospho-dependent prolyl cis ⁄ trans isomerase Pin1 docx

Báo cáo khoa học: Molecular mechanisms of the phospho-dependent prolyl cis ⁄ trans isomerase Pin1 docx

... novel inter- action surfaces for two distinct molecular partners [6], and the conformation of a proline in the linker region between two SH3 domains of the Crp adaptor protein determines the autoinhibition ... detected in vitro of CypA on Pro90 in the full- length Gag protein [5] plays a role in the viral restriction process in vivo remains an open question. I...
Ngày tải lên : 07/03/2014, 05:20
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Báo cáo khoa học: Molecular characterization of Osh6p, an oxysterol binding protein homolog in the yeast Saccharomyces cerevisiae pot

Báo cáo khoa học: Molecular characterization of Osh6p, an oxysterol binding protein homolog in the yeast Saccharomyces cerevisiae pot

... to the Golgi for further glycosylation (p2 form with molecular mass of 69 kDa). The p2 form of CPY traverses the Golgi and is packaged into vesi- cles destined for the vacuolar protein sorting ... motif for pro- tein–protein interaction. In this study, Osh6p was demonstrated to bind a pool of phosphatidylinositol phosphates (PtdInsP) including PtdIns(4)P, PtdIns(5)P, P...
Ngày tải lên : 07/03/2014, 21:20
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Báo cáo khoa học: Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis Functional analysis of the promoter identifies a calcium sensitive region required for basal activity doc

Báo cáo khoa học: Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis Functional analysis of the promoter identifies a calcium sensitive region required for basal activity doc

... sites within the MGP coding sequence (Fig. 2 ). Analysis of the phase of each of the xMGP introns located within the coding region revealed that introns 2 and 3 are of phase I while intron 4 is ... is split into two exons (IA and IB) in the X. laevis gene, with the site of the intron insertion localized within the 5¢ UTR region of t he xMGP gene (Fig. 1 and Table...
Ngày tải lên : 08/03/2014, 10:20
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Báo cáo khoa học: Molecular basis of toxicity of Clostridium perfringens epsilon toxin ppt

Báo cáo khoa học: Molecular basis of toxicity of Clostridium perfringens epsilon toxin ppt

... compro- mises the intestinal barrier of intoxicated animals, allowing the dissemination of toxin via the bloodstream to the main target organs of the kidneys and brain. The mechanism of e-toxin absorption ... that toxin-induced oedema of the brain is due to the damaging action of the toxin on vascular endothelial cells [45]. Toxin- induced increase in vascular...
Ngày tải lên : 14/03/2014, 22:20
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Báo cáo khoa học: Molecular basis of cytokine signalling – theme and variations docx

Báo cáo khoa học: Molecular basis of cytokine signalling – theme and variations docx

... N-terminal extension (clip) binds into the epitope for the type I receptor of the ligand, whereas the main core structure binds into the epitope for the type II receptor of the BMP ligand. Therefore, ... a binding site for the tyrosine kinase Janus kinase 3 (JAK3), which transactivates. The driver, IL-4Ra, contains a large cytosolic domain with binding motifs...
Ngày tải lên : 15/03/2014, 09:20
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Báo cáo khoa học: Molecular dynamics of the DNA-binding domain of the papillomavirus E2 transcriptional regulator uncover differential properties for DNA target accommodation pdf

Báo cáo khoa học: Molecular dynamics of the DNA-binding domain of the papillomavirus E2 transcriptional regulator uncover differential properties for DNA target accommodation pdf

... the deformability required for the formation of the Early protein 2 C-terminal DNA-binding domain–DNA complexes of var- ious types is based not only on the rigidity of the base sequences in the DNA ... to changes in the DNA recognition kinetics and in the stability of the complex [22], suggesting that there is an involvement of this loop in HPV-16 E2–DNA...
Ngày tải lên : 16/03/2014, 10:20
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Báo cáo khoa học: Identification of the structural determinant responsible for the phosphorylation of G-protein activated potassium channel 1 by cAMP-dependent protein kinase pdf

Báo cáo khoa học: Identification of the structural determinant responsible for the phosphorylation of G-protein activated potassium channel 1 by cAMP-dependent protein kinase pdf

... that the failure of these seven mutations to abolish protein phosphorylation was a result of other protein kinases masking the PKA-catalysed part. Indeed, protein kinases other than PKA, including tyrosine ... assess the role of the S ⁄ Ts in the regulation of GIRK1 via PKA in a manner that is unbiased by the eventual contributions of the other subunits in a het-...
Ngày tải lên : 07/03/2014, 00:20
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