... structures of the
wild-type, single- and double-mutant EhPGKs in a
closed conformation will certainly help to clarify the
molecular arrangement in the guanine ring binding site
during the binding of ... carbon 2 of the guanine ring may interact with
the side chain of Glu309, whereas the carbonyl group at
position 6 of the guanine ring may interact with the
hy...
... soluble
version of the mutant protein. The K
cat
⁄ K
m
value of
the mutant TNSALP was less than one-tenth of the
K
cat
⁄ K
m
value of the soluble form of the wild-type
enzyme, indicating that the replacement ... resulting in
a K
cat
⁄ K
m
value that was < 10% of that of the wild-
type enzyme (Table 1), indicating that the conversion
of valine to alanine...
... An in silico docking
analysis of glyphosate binding at the GO active site
showed that glyphosate is bound in the same orienta-
tion as inferred for glycine (with the phosphonate moi-
ety pointing ... in the binding energy for this small
substrate. Because of the introduction of an arginine
at position 54, the a2–a3 loop (comprising resi-
dues 50–60) assumes a diffe...
... novel inter-
action surfaces for two distinct molecular partners [6],
and the conformation of a proline in the linker region
between two SH3 domains of the Crp adaptor protein
determines the autoinhibition ... detected in vitro of CypA on Pro90 in the full-
length Gag protein [5] plays a role in the viral
restriction process in vivo remains an open question. I...
... to the Golgi for further glycosylation
(p2 form with molecular mass of 69 kDa). The p2 form
of CPY traverses the Golgi and is packaged into vesi-
cles destined for the vacuolar protein sorting ... motif for pro-
tein–protein interaction. In this study, Osh6p was
demonstrated to bind a pool of phosphatidylinositol
phosphates (PtdInsP) including PtdIns(4)P, PtdIns(5)P,
P...
... sites within the MGP coding sequence
(Fig. 2 ). Analysis of the phase of each of the xMGP introns
located within the coding region revealed that introns 2 and
3 are of phase I while intron 4 is ... is split into two exons (IA and IB) in the
X. laevis gene, with the site of the intron insertion localized
within the 5¢ UTR region of t he xMGP gene (Fig. 1 and
Table...
... compro-
mises the intestinal barrier of intoxicated animals,
allowing the dissemination of toxin via the bloodstream
to the main target organs of the kidneys and brain. The
mechanism of e-toxin absorption ... that toxin-induced
oedema of the brain is due to the damaging action of
the toxin on vascular endothelial cells [45]. Toxin-
induced increase in vascular...
... N-terminal extension (clip) binds into the
epitope for the type I receptor of the ligand, whereas the main core structure binds into the epitope for the type II receptor of the BMP
ligand. Therefore, ... a
binding site for the tyrosine kinase Janus kinase 3
(JAK3), which transactivates. The driver, IL-4Ra,
contains a large cytosolic domain with binding motifs...
... the deformability required for the formation of the
Early protein 2 C-terminal DNA-binding domain–DNA complexes of var-
ious types is based not only on the rigidity of the base sequences in the
DNA ... to
changes in the DNA recognition kinetics and in the
stability of the complex [22], suggesting that there is
an involvement of this loop in HPV-16 E2–DNA...
... that the failure of these seven mutations to
abolish protein phosphorylation was a result of other
protein kinases masking the PKA-catalysed part.
Indeed, protein kinases other than PKA, including
tyrosine ... assess the role of the S ⁄ Ts in the regulation of
GIRK1 via PKA in a manner that is unbiased by the
eventual contributions of the other subunits in a het-...