Báo cáo khoa học: Probing the catalytic potential of the hamster arylamine N-acetyltransferase 2 catalytic triad by site-directed mutagenesis of the proximal conserved residue, Tyr190 pot

Báo cáo khoa học: Probing the catalytic potential of the hamster arylamine N-acetyltransferase 2 catalytic triad by site-directed mutagenesis of the proximal conserved residue, Tyr190 pot

Báo cáo khoa học: Probing the catalytic potential of the hamster arylamine N-acetyltransferase 2 catalytic triad by site-directed mutagenesis of the proximal conserved residue, Tyr190 pot

... ª 20 09 FEBS 6941 Probing the catalytic potential of the hamster arylamine N-acetyltransferase 2 catalytic triad by site-directed mutagenesis of the proximal conserved residue, Tyr190 Xin Zhou 1 , ... refolding [26 ,27 ]. Conse- quently, we hypothesized that modulation of the cata- lytic potential of the catalytic triad might be accessi...
Ngày tải lên : 16/03/2014, 00:20
  • 14
  • 350
  • 0
Tài liệu Báo cáo khoa học: A knowledge-based potential function predicts the specificity and relative binding energy of RNA-binding proteins ppt

Tài liệu Báo cáo khoa học: A knowledge-based potential function predicts the specificity and relative binding energy of RNA-binding proteins ppt

... 3.94 )0.68 )2. 09 0. 42 2ADB 2. 66 0.37 )2. 15 0.16 2ADC 3.01 )0 .21 )0.65 0.63 2ASB 2. 12 )0.94 )2. 07 0.48 2ATW 2. 19 )1.38 )2. 94 0.33 2CJK 2. 75 1. 42 2.47 0. 42 Mean ± SD )0.69 ± 1 .28 )0.59 ± 1.94 0.41 ... 0.37 1CVJ 2. 63 )1.13 )0.44 0.51 1DFU 2. 45 )1. 82 )1.93 0.39 1E7K 2. 58 )0.38 0.83 0.34 1EC6 5 .21 )3. 02 )2. 29 0.71 1FJE 3.54 1.49 2. 51 0. 42 1FXL 2. 22 )1. 32 )...
Ngày tải lên : 18/02/2014, 16:20
  • 14
  • 736
  • 0
Tài liệu Báo cáo khoa học: Probing the molecular determinants of aniline dioxygenase substrate specificity by saturation mutagenesis docx

Tài liệu Báo cáo khoa học: Probing the molecular determinants of aniline dioxygenase substrate specificity by saturation mutagenesis docx

... residues H204, H209 and D356 correspond to the catalytic facial triad that coordinates the mono- nuclear iron in the active site (H208, H213 and D3 62 of NDO), whereas D201 corresponds to D205 of NDO, ... nmolÆmin )1 Æmg )1 protein. On the other hand, the 24 DMA activity of the V205A mutant was reduced to 0.1 nmolÆmin )1 Æmg )1 protein (Table 2) . The 24 DMA conver...
Ngày tải lên : 19/02/2014, 02:20
  • 12
  • 634
  • 0
Tài liệu Báo cáo khoa học: "Probing the lexicon in evaluating commercial MT systems Martin" pot

Tài liệu Báo cáo khoa học: "Probing the lexicon in evaluating commercial MT systems Martin" pot

... context. But the results of this study cannot be used for comparing the sizes of lexicons since the number of error tokens is given rather than the number of error types. Furthermore it is ... 90/ 72/ 21 98/80/30 97/63/16 Lang. T1 97/74 /28 100/83/44 97/85 /26 Personal Tr. 99/ 92/ 69 100/94/73 99/91/67 Power Tr. 92/ 75/43 98/77/44 100/76 /22 Systran 97/43 /...
Ngày tải lên : 22/02/2014, 03:20
  • 8
  • 320
  • 0
Báo cáo khoa học: Probing the unfolding region of ribonuclease A by site-directed mutagenesis potx

Báo cáo khoa học: Probing the unfolding region of ribonuclease A by site-directed mutagenesis potx

... ) 0.6 ± 0.4 S21L 2. 5 ± 0 .2 2.78 ± 0. 02 16.4 ± 1.8 0 .2 ± 0.5 1.8 ± 0.4 A20P/S21P 2. 8 ± 0.4 2. 82 ± 0. 02 11.1 ± 0.8 ) 0.3 ± 0.5 ) 1.0 ± 1.6 N34D 2. 9 ± 0.1 2. 86 ± 0. 02 13.1 ± 1 .2 ) 0.9 ± 0.5 ) 0.9 ... investigate the contribution of the two loop regions to the stability of the entire RNase A molecule, we replaced several amino-acid residues b y site-directed...
Ngày tải lên : 16/03/2014, 18:20
  • 10
  • 491
  • 0
Báo cáo khoa học: Probing the mechanism of the bifunctional enzyme ketol-acid reductoisomerase by site-directed mutagenesis of the active site pot

Báo cáo khoa học: Probing the mechanism of the bifunctional enzyme ketol-acid reductoisomerase by site-directed mutagenesis of the active site pot

... 66 13.6 ± 0.7 624 4 ± 431 7 .27 ± 0.44 K155E ND 2. 66 ± 0 .23 23 .3 ± 2. 9 8.04 ± 1.14 K155Q ND 15.3 ± 6.0 9.78 ± 0. 42 9.30 ± 2. 11 E213D 922 ± 91 3.67 ± 0.70 20 79 ± 24 5 16.0 ± 2. 0 E213Q ND 0.441 ± ... ND D217E ND ND ¥ a 80.0 ± 12. 0 a D217N b ND 7.64 ± 0.68 114 ± 7 5.08 ± 0. 52 E 221 D 356 ± 18 1.37 ± 0.15 20 38 ± 171 20 .3 ± 2. 8 E 221 Q ND ND 470 ± 100 a 20 .0 ± 3.0 a E389...
Ngày tải lên : 16/03/2014, 18:20
  • 10
  • 339
  • 0
Báo cáo khoa học: Probing the interface between factor Xa and tissue factor in the quaternary complex tissue factor–factor VIIa–factor Xa–tissue factor pathway inhibitor pptx

Báo cáo khoa học: Probing the interface between factor Xa and tissue factor in the quaternary complex tissue factor–factor VIIa–factor Xa–tissue factor pathway inhibitor pptx

... Copenhagen, Denmark. (Received 7 February 20 03, revised 15 April 20 03, accepted 17 April 20 03) Eur. J. Biochem. 27 0, 25 76 25 82 (20 03) Ó FEBS 20 03 doi:10.1046/j.14 32- 1033 .20 03.03 625 .x [8,9,11–16], and the same area has ... to each other in an area of the C-terminal part of the TF1 domain and the N-terminal portion of the TF2 domain (Fig. 1). Mutation and la...
Ngày tải lên : 17/03/2014, 03:20
  • 7
  • 450
  • 0
Báo cáo khoa học: Probing suggested catalytic domains of glycosyltransferases by site-directed mutagenesis Tobias Hefner and Joachim Stockigt ¨ ppt

Báo cáo khoa học: Probing suggested catalytic domains of glycosyltransferases by site-directed mutagenesis Tobias Hefner and Joachim Stockigt ¨ ppt

... (GENE:AT2G2 326 0) (A. thaliana, O 221 82) , AT II: putative glucosyltransferase (GENE:AT2G2 325 0) (A. thaliana, O 221 83), AT III: putative glucosyltransferase (GENE:AT2G2 321 0) (A. thaliana, O 221 86), ... 440 46.5 20 2.9 24 .1 2. 59 5886.4 L86I 4 52 38.3 180.4 20 .4 1.84 4070.8 A204V 706 12. 3 139.1 13.1 1.30 1841.4 E368D 411 1.9 17.8 0.9 0. 12 2 92. 0 E368A 398 6 .2 24.0 1.1 0.08...
Ngày tải lên : 17/03/2014, 09:20
  • 6
  • 282
  • 0
Báo cáo khoa học: Probing plasma clearance of the thrombin–antithrombin complex with a monoclonal antibody against the putative serpin–enzyme complex receptor-binding site docx

Báo cáo khoa học: Probing plasma clearance of the thrombin–antithrombin complex with a monoclonal antibody against the putative serpin–enzyme complex receptor-binding site docx

... each. The error bars represent the range. (B) HepG2 cells were incubated with 20 0 lLof20n M 125 I-T–AT in the absence or presence of 2 l M unlabeled T–AT, 0 .2 l M Fab M27, 0 .2 l M mAb M27, 0 .2 l M mAbM38,and1l M cleaved ... that the sequence including FIREVP (residues 411–416) constitutes a critical part of the linear epitope of mAb M27. Effect of M27 on inhibiti...
Ngày tải lên : 17/03/2014, 10:20
  • 11
  • 378
  • 0
Báo cáo khoa học: Probing the substrate specificities of matriptase, matriptase-2, hepsin and DESC1 with internally quenched fluorescent peptides potx

Báo cáo khoa học: Probing the substrate specificities of matriptase, matriptase-2, hepsin and DESC1 with internally quenched fluorescent peptides potx

... specificities of TTSPs F. Be ´ liveau et al. 22 22 FEBS Journal 27 6 (20 09) 22 13 22 26 ª 20 09 The Authors Journal compilation ª 20 09 FEBS to the medium, and the cells were incubated for a 2- week selection ... specificities of TTSPs F. Be ´ liveau et al. 22 26 FEBS Journal 27 6 (20 09) 22 13 22 26 ª 20 09 The Authors Journal compilation ª 20 09 FEBS Probing the s...
Ngày tải lên : 23/03/2014, 04:21
  • 14
  • 279
  • 0

Xem thêm

Từ khóa: