Báo cáo khoa học: Probing the catalytic potential of the hamster arylamine N-acetyltransferase 2 catalytic triad by site-directed mutagenesis of the proximal conserved residue, Tyr190 pot
... ª 20 09 FEBS 6941
Probing the catalytic potential of the hamster arylamine
N-acetyltransferase 2 catalytic triad by site-directed
mutagenesis of the proximal conserved residue, Tyr190
Xin Zhou
1
, ... refolding [26 ,27 ]. Conse-
quently, we hypothesized that modulation of the cata-
lytic potential of the catalytic triad might be accessi...
... residues H204, H209 and D356 correspond
to the catalytic facial triad that coordinates the mono-
nuclear iron in the active site (H208, H213 and D3 62
of NDO), whereas D201 corresponds to D205 of
NDO, ... nmolÆmin
)1
Æmg
)1
protein. On the other hand,
the 24 DMA activity of the V205A mutant was
reduced to 0.1 nmolÆmin
)1
Æmg
)1
protein (Table 2) .
The 24 DMA conver...
... context. But the results
of this study cannot be used for comparing the sizes
of lexicons since the number of error tokens is given
rather than the number of error types. Furthermore
it is ...
90/ 72/ 21
98/80/30
97/63/16
Lang. T1
97/74 /28
100/83/44
97/85 /26
Personal Tr.
99/ 92/ 69
100/94/73
99/91/67
Power Tr.
92/ 75/43
98/77/44
100/76 /22
Systran
97/43 /...
... Copenhagen,
Denmark.
(Received 7 February 20 03, revised 15 April 20 03,
accepted 17 April 20 03)
Eur. J. Biochem. 27 0, 25 76 25 82 (20 03) Ó FEBS 20 03 doi:10.1046/j.14 32- 1033 .20 03.03 625 .x
[8,9,11–16], and the same area has ... to
each other in an area of the C-terminal part of the TF1
domain and the N-terminal portion of the TF2 domain
(Fig. 1). Mutation and la...
... each. The error bars
represent the range. (B) HepG2 cells were incubated with 20 0 lLof20n
M
125
I-T–AT in the absence or presence of 2 l
M
unlabeled T–AT, 0 .2 l
M
Fab M27, 0 .2 l
M
mAb M27, 0 .2 l
M
mAbM38,and1l
M
cleaved ... that the sequence including FIREVP
(residues 411–416) constitutes a critical part of the linear
epitope of mAb M27.
Effect of M27 on inhibiti...
... specificities of TTSPs F. Be
´
liveau et al.
22 22 FEBS Journal 27 6 (20 09) 22 13 22 26 ª 20 09 The Authors Journal compilation ª 20 09 FEBS
to the medium, and the cells were incubated for a 2- week
selection ... specificities of TTSPs F. Be
´
liveau et al.
22 26 FEBS Journal 27 6 (20 09) 22 13 22 26 ª 20 09 The Authors Journal compilation ª 20 09 FEBS
Probing the s...