... Requirement for asparagine in the aquaporin NPA
sequence signature motifs for cation exclusion
Dorothea Wree
1
, Binghua Wu
1
, Thomas Zeuthen
2
and Eric Beitz
1
1 Department ... highly conserved NPA motifs are a hallmark of the aquaporin (AQP)
family. The NPA triplets form N-terminal helix capping structures with the
Asn side chains located in the...
... that the structure of the
N-terminal domain is maintained, at least in the
vicinity of the tryptophan residues. The increase in
exposed clustered hydrophobicity observed for the
mutants is therefore ... renders the pro-
tein unable to form oligomers [58], indicating that
interactions involving the IXI motif are also essential
for the oligomerization of mammalian sHsp...
... [15,22];
and (b) the domain is stable under the conditions used
in SPR experiments.
Assessment of optimal conditions for studying
the Hsc70–NBD1 interaction by SPR
Before determining the effect of the F508del ... indicate
that we can use SPR to assay the binding of interac-
ting proteins to NBD1. To further optimize the condi-
tions for investigating the NBD1–Hsc70 inter...
... against HNE–protein adducts. Ligand-
binding tests showing the functionality of the same HNE-treated porcine (B) and bovine (C) OBPs as in the immunostaining. The plots show
the residual AMA-binding ... frequently. For example, bovine
OBP is peculiar in that it is a dimer with domain
swapping [14,15]. There is currently a lack of informa-
tion on the in vivo binding proper...
... [10,14],
showed the same strong binding affinity. Further-
more, major changes of the surrounding sequences
did not affect the binding affinity of the recognition
sequence, indicating that it is generally independent
of ... binding mode of the HD and in uence the
nucleotide contacts made by the recognition helix [6].
Notably, these different binding modes seemed to
depend on th...
... retinoid-binding pro-
teins between photoreceptors and retinal pigment epi-
thelium (RPE); it is essential for maintaining normal
vision [3,4]. The key step in the retinoid visual cycle is
the ... RPE65c in the inner retina,
including Mu
¨
ller cells, but not in the RPE. Furthermore, double-immuno-
staining of dissociated retinal cells using antibodies for RPE65c and gluta...
... 2007)
doi:10.1111/j.1742-4658.2007.06051.x
The mechanisms underlying increased cardioprotection in younger female
mice are unclear. We hypothesized that serine-threonine protein kinase
(protein kinase B; Akt) triggers ... effects via the prosurvival serine-threonine
protein kinase, Akt (also known as protein kinase B)
[2]. In agreement with this, elevated levels of activated
Akt in...
... integrin-dependent
cell volume sensing and signaling integrates into the
overall context of insulin signaling. Similarly, sensing
of glutamine-induced hepatocyte swelling by integrins
feeds into ... hepatocyte swelling [12,13], insu-
lin-induced hepatocyte swelling is registrated by the
integrin system, leading to a Src-dependent activation
of the p38-type MAPK and thereby inhibition of...
... of the c ring, respectively. The
C-terminal helices show a clear handedness, and two
of the rings face in the opposite direction in the
membrane to the other two, forming the same pattern
as in ... of the
ion-binding site. Therefore, the c ring of A. woodii has
only 10 membrane-buried negative charges that are
essential for binding the ion and also for the rota...
... synthesized in the Spemann
organizer, namely Follistatin, Chordin and Noggin
[12]. These proteins are antagonists of BMP and other
members of the TGF-b family, binding to and inhibit-
ing these ... these signaling molecules from binding to their
receptors in the extracellular space, thus inhibiting ven-
tralizing activities. Of these proteins, Chordin has a
long-range effect. In X...