Báo cáo khoa học: Requirement for asparagine in the aquaporin NPA sequence signature motifs for cation exclusion docx

Báo cáo khoa học: Requirement for asparagine in the aquaporin NPA sequence signature motifs for cation exclusion docx

Báo cáo khoa học: Requirement for asparagine in the aquaporin NPA sequence signature motifs for cation exclusion docx

... Requirement for asparagine in the aquaporin NPA sequence signature motifs for cation exclusion Dorothea Wree 1 , Binghua Wu 1 , Thomas Zeuthen 2 and Eric Beitz 1 1 Department ... highly conserved NPA motifs are a hallmark of the aquaporin (AQP) family. The NPA triplets form N-terminal helix capping structures with the Asn side chains located in the...
Ngày tải lên : 15/03/2014, 00:20
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Báo cáo khoa học: Glutamic acid residues in the C-terminal extension of small heat shock protein 25 are critical for structural and functional integrity pptx

Báo cáo khoa học: Glutamic acid residues in the C-terminal extension of small heat shock protein 25 are critical for structural and functional integrity pptx

... that the structure of the N-terminal domain is maintained, at least in the vicinity of the tryptophan residues. The increase in exposed clustered hydrophobicity observed for the mutants is therefore ... renders the pro- tein unable to form oligomers [58], indicating that interactions involving the IXI motif are also essential for the oligomerization of mammalian sHsp...
Ngày tải lên : 07/03/2014, 04:20
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Báo cáo khoa học: Deletion of Phe508 in the first nucleotide-binding domain of the cystic fibrosis transmembrane conductance regulator increases its affinity for the heat shock cognate 70 chaperone docx

Báo cáo khoa học: Deletion of Phe508 in the first nucleotide-binding domain of the cystic fibrosis transmembrane conductance regulator increases its affinity for the heat shock cognate 70 chaperone docx

... [15,22]; and (b) the domain is stable under the conditions used in SPR experiments. Assessment of optimal conditions for studying the Hsc70–NBD1 interaction by SPR Before determining the effect of the F508del ... indicate that we can use SPR to assay the binding of interac- ting proteins to NBD1. To further optimize the condi- tions for investigating the NBD1–Hsc70 inter...
Ngày tải lên : 23/03/2014, 05:22
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Báo cáo khoa học: Odorant binding protein has the biochemical properties of a scavenger for 4-hydroxy-2-nonenal in mammalian nasal mucosa doc

Báo cáo khoa học: Odorant binding protein has the biochemical properties of a scavenger for 4-hydroxy-2-nonenal in mammalian nasal mucosa doc

... against HNE–protein adducts. Ligand- binding tests showing the functionality of the same HNE-treated porcine (B) and bovine (C) OBPs as in the immunostaining. The plots show the residual AMA-binding ... frequently. For example, bovine OBP is peculiar in that it is a dimer with domain swapping [14,15]. There is currently a lack of informa- tion on the in vivo binding proper...
Ngày tải lên : 23/03/2014, 10:20
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Báo cáo khoa học: Autoregulatory binding sites in the zebrafish six3a promoter region define a new recognition sequence for Six3 proteins potx

Báo cáo khoa học: Autoregulatory binding sites in the zebrafish six3a promoter region define a new recognition sequence for Six3 proteins potx

... [10,14], showed the same strong binding affinity. Further- more, major changes of the surrounding sequences did not affect the binding affinity of the recognition sequence, indicating that it is generally independent of ... binding mode of the HD and in uence the nucleotide contacts made by the recognition helix [6]. Notably, these different binding modes seemed to depend on th...
Ngày tải lên : 29/03/2014, 08:20
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Tài liệu Báo cáo khoa học: An alternative isomerohydrolase in the retinal Muller cells of a cone-dominant species doc

Tài liệu Báo cáo khoa học: An alternative isomerohydrolase in the retinal Muller cells of a cone-dominant species doc

... retinoid-binding pro- teins between photoreceptors and retinal pigment epi- thelium (RPE); it is essential for maintaining normal vision [3,4]. The key step in the retinoid visual cycle is the ... RPE65c in the inner retina, including Mu ¨ ller cells, but not in the RPE. Furthermore, double-immuno- staining of dissociated retinal cells using antibodies for RPE65c and gluta...
Ngày tải lên : 14/02/2014, 14:20
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Tài liệu Báo cáo khoa học: Metabolic gene switching in the murine female heart parallels enhanced mitochondrial respiratory function in response to oxidative stress pdf

Tài liệu Báo cáo khoa học: Metabolic gene switching in the murine female heart parallels enhanced mitochondrial respiratory function in response to oxidative stress pdf

... 2007) doi:10.1111/j.1742-4658.2007.06051.x The mechanisms underlying increased cardioprotection in younger female mice are unclear. We hypothesized that serine-threonine protein kinase (protein kinase B; Akt) triggers ... effects via the prosurvival serine-threonine protein kinase, Akt (also known as protein kinase B) [2]. In agreement with this, elevated levels of activated Akt in...
Ngày tải lên : 18/02/2014, 16:20
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Tài liệu Báo cáo khoa học: Osmosensing and signaling in the regulation of mammalian cell function docx

Tài liệu Báo cáo khoa học: Osmosensing and signaling in the regulation of mammalian cell function docx

... integrin-dependent cell volume sensing and signaling integrates into the overall context of insulin signaling. Similarly, sensing of glutamine-induced hepatocyte swelling by integrins feeds into ... hepatocyte swelling [12,13], insu- lin-induced hepatocyte swelling is registrated by the integrin system, leading to a Src-dependent activation of the p38-type MAPK and thereby inhibition of...
Ngày tải lên : 18/02/2014, 16:20
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Tài liệu Báo cáo khoa học: An intermediate step in the evolution of ATPases – a hybrid F0–V0 rotor in a bacterial Na+ F1F0 ATP synthase pdf

Tài liệu Báo cáo khoa học: An intermediate step in the evolution of ATPases – a hybrid F0–V0 rotor in a bacterial Na+ F1F0 ATP synthase pdf

... of the c ring, respectively. The C-terminal helices show a clear handedness, and two of the rings face in the opposite direction in the membrane to the other two, forming the same pattern as in ... of the ion-binding site. Therefore, the c ring of A. woodii has only 10 membrane-buried negative charges that are essential for binding the ion and also for the rota...
Ngày tải lên : 18/02/2014, 17:20
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Tài liệu Báo cáo khoa học: Bone morphogenetic proteins in the early development of zebrafish pptx

Tài liệu Báo cáo khoa học: Bone morphogenetic proteins in the early development of zebrafish pptx

... synthesized in the Spemann organizer, namely Follistatin, Chordin and Noggin [12]. These proteins are antagonists of BMP and other members of the TGF-b family, binding to and inhibit- ing these ... these signaling molecules from binding to their receptors in the extracellular space, thus inhibiting ven- tralizing activities. Of these proteins, Chordin has a long-range effect. In X...
Ngày tải lên : 19/02/2014, 00:20
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