... the program
GNOMOKO
[24]. The
molecular masses were calculated from the ratio of
the forward scattering intensity of the samples and of the
molecular mass standard BSA. The volume fractions of
monomers, ... large amounts of indole-3-acetic acid.
Indolepyruvate decarboxylase, the key enzyme in the
biosynthetic pathway of indole-3-acetic acid, catalyses the
for...
... calculated from the
measured relaxation parameters, that contain contribu-
tions from the overall as well as the local dynamics.
Graphical analysis of the spectral density values pro-
vides a ... the
similar values of the mean and weighed mean s
e
.
Helix II shows the largest variability in local fluctua-
tion frequency, despite the fact that the relative mean
generaliz...
... for the catalytic modules of other
polymerases [11]. Furthermore, the conservation of
catalytic aspartate residues and their 3D arrangement
suggest that the catalysis mode is probably comparable
with ... domain performs no primase
activity; and (b) after the best possible fit of the Ca
atoms of the catalytic triad, are positional homologs
of the R148 and K300 residues...
... years as a result of
the expansion of the Aedes aegypti mosquito to dif-
ferent geographic areas, and DHF has spread from
South East Asia to the Western Pacific and the
Americas. A substantial ... epitopes of
the others [63].
The major pharmaceutical companies are currently
developing a treatment against the disease. A tetra-
valent live attenuated vaccine was devel...
... of the 5¢ exon, and 25 bp
of the 3¢ exon. Oligo 104 (45 nucleotides, TAATACGACTC
ACTATAGGGATCGAATTCTGGGTTCAAAACGTAA)
contains, in order, a T7 RNA polymerase promoter (nucleo-
tides 1–17), a six-nucleotide ... transcription enhancer, an
EcoRI restriction site, 14 nucleotides of authentic 5¢ exon,
and the first two nucleotides of the Cr.LSU intron. Oligo 158
(26 nucleotides,...
... matching
with the C-terminal sequence of the coding region: 5¢TTAC
AAGGACAAATTAATTGTGCCAG. For amplification
of the long isoform the same 5¢ primers were used, the 3¢
specific primer was FF3B: 5¢TTACAAGTCTTGCAA
AGGGAAGGAT. ... subtraction
of the spontaneous release of the basophils, the allergen-
induced histamine release was calculated as percent of the
total amount...
... peptides was proposed as the reason of
this apparent increased uptake, as the rate of uptake was
thesameinserum-freemedium[17].Asalreadystated
above, this Tat CPP peptide is able to vectorize various
cargo ... T at peptide uptake i s
under evaluation.
Comparative FACS analysis of the internalization
of the full-length Tat protein construct and the Tat CPP
Differences in the...
... signal
of the preceding amino acid, correlating the amide HN and
the Ca signals, correlating the amide HN and the Ca signal
of the preceding amino acid, correlating the amide NH with
the Ca and ... of the protein backbone, and for
more than 78% of the side chain atoms.
The main set of backbone u and w dihedral angles was
calculated from the chemical shift val...