Báo cáo khoa học: Subunit sequences of the 4 · 6-mer hemocyanin from the golden orb-web spider, Nephila inaurata Intramolecular evolution of the chelicerate hemocyanin subunits pot
... Subunit sequences of the 4 · 6-mer hemocyanin from the golden
orb-web spider,
Nephila inaurata
Intramolecular evolution of the chelicerate hemocyanin subunits
Anne Averdam, ... the 4 · 6-mer hemocyanin
requires the stoichiometric association of all seven subunit
types (4 · a, 2· b, 2· c ,4 d ,4 e ,4 f ,4 g), with
each...
... AJ 543 425 (hpdB), AJ 543 426 (hpdC)and
AJ 543 427 (hpdA). Within the hpdB gene, nine nucleotides
were exchanged between the type strain and strain 630, but
only two of these replacements changed the ... allow
in-frame cloning of the genes. While the BamHI sites
introduced in the 3¢-UTR of the genes did not affect the
resulting amino acid sequences, the introduction...
... transcriptional regulation of the human
S5a gene, we used PCR to isolate 1.2 kb of HeLa
genomic DNA upstream of the first exon of the
A
B
C
D
E
F
24h 48 h 72h 96h
Regulation of subunit S5a of the 26S proteasome ... factor of the Wnt
signaling cascade, was present at position ) 244 of the
promoter. The Wnt pathway regulates the ability of
the proto-oncogen...
... a
FEBS Journal 272 (2005) 46 31 46 45 ª 2005 FEBS 46 41
extinction coefficient (A
280
) of 52501 cm
)1
Æm
)1
, following
the method of Gill and von Hippel [46 ]. The homogeneity
of the protein was routinely ... Journal 272 (2005) 46 31 46 45 ª 2005 FEBS 46 43
Murcko MA & Su MS (1997) The structural basis for
the specificity of pyridinylimidazole inhibitors of p38
MAP...
... lever-
aging the functionality and density properties of
sequences as done in our SEQ system.
286
Pattern Example
the ORD the fifth
the RB ORD the very first
the JJS the best
the RB JJS the very best
the ... presidents of the United
States or the most common causes of death
in the U.S. SEQ leverages regularities
about sequences to extract a coherent set
of...
... lowers the intracellular levels of benzoate and fluorescein
by catalysing an active efflux of these compounds from the
cell [2 ,4] . The inhibitory effects of different organic acids on
cells of the ... Sal1-digested and cloned into the
SalI site located between the ADH1 promoter and termi-
nator of expression vector pAD4M [ 14] . The correct DNA
sequence of the TA...
... requires further
investigation. In light of the apoptotic effect of PPARc
in adipocytes, we conclude that PPARc is one of the
key regulators involved in the physiology of the adipo-
cyte, from its ... and
30870559 from the China National Nature Sciences
Foundation, 2006CB910703 from the Ministry of
Sciences and Technology of China, and 07dz05907
from the Comm...
... determined the effect of AG 147 8
on the levels of MAPK phosphatases MKP-1 and
MKP-2. As shown in Fig. 4B, AG 147 8 decreased the
expression of the MKP-1 protein. As for the MKP-2
protein, however, AG 147 8 ... examined the effect of AG 147 8 on the viabil-
ity of human NSCLC cell line PC-9. Treatment of the
cells with AG 147 8 markedly suppressed the cell viabil...
... by PCR from the pfVEGF
construct [15], into pGEM4Z (Promega). The pGEM 44,
46 and 47 constructs contain, respectively, mouse VEGF
5¢-UTR sequences +1 to +325, +46 1 to +727 and +735 to
+10 14 (relative ... composed of the entire VEGF
mouse 5¢-UTR (+1 to +10 14) , the coding region for the 1 64
aminoacidformofVEGFandtheVEGF3¢-UTR ( +4 to
+2195). The major polyadenylation...