... at the
same time, distinct absorption bands of oxyheme
appeared at 540 and 579 nm. Then, a broad band
appeared at around 660 nm, and was maximal 9–12 min
after initiation of the reaction. The ... heme–GmHO-1 initiates
the reaction, as revealed by gradual diminution of the
Soret band. After several minutes, a broad band
appears at around 660–675 nm; this increases in inte...
... sites. In the case of the titin and twitchin
kinases, the autoinhibitory sequence acts as a pseudo-
substrate, occluding ATP binding and preventing
protein substrates from binding (reviewed in [9]).
PhK ... An intrinsic calmodulin (CaM, the d
subunit) binds directly to the c protein kinase chain. The interaction site
of CaM on c has been localized to a C-terminal ext...
... thioester acyl–enzyme
intermediate. Biochemistry 49, 341–346.
3 Fukuda A, Matsuyama S, Hara T, Nakayama J,
Nagasawa H & Tokuda H (2002) Aminoacylation of
the N-terminal cysteine is essential for ... than that
from RN4220, MW2 or MSSA476 strain, indicating
that shorter fatty acids were mainly attached to the tria-
cylated lipopeptides of SitC of the SA113 strain
(Figs 1A an...
... macromolecule-64
(OMM-64), that is contained in a HMW aggregate
in the otolith matrix. During characterization of this
protein, we revealed that the aggregate also contains the
inner ear-specific collagen otolin-1 [9].
Results
Cloning ... However, the structural and biochemical
bases of the biomineralization framework and mineral-
associated protein have not been elucid...
... staphy-
lococcal adhesins, comprising an N-terminal region that binds fibrinogen
and elastin, and a C-terminal domain that interacts with fibronectin. The
C-terminal domain of fibronectin-binding ... FEBS
Monoclonal antibodies against FnBRs of FnBPB
A panel of mouse mAbs was produced against the
recombinant repetitive region of FnBPB. Analysis of
mAbs binding to the recombina...
... PCR with GAGCGG
CATATGGA
AATCAAACCGAAGGTTCGCGA and GAGCGG
CATA
TGGAAATCAAACCGAAGGTTCGCGA as the forward
and reverse oligonucleotide primers, respectively. The
primers were designed to introduce ... coordi-
nated by an oxygen donor group derived from the
carboxylate side chain of either a glutamate or an
aspartate. The apparent conflict of this finding with
the absence of a...
... [46]. Other sta-
tistical approaches analysing structural parameters in
large samples of dissimilar proteins regarding the origin
and temperature range, do not show significant trends
regarding the ... adequate to maintain their active
conformation.
In order to improve the understanding of the struc-
tural principles of temperature adaptation we studied a
subtilisin-like...
... resultant binding and selective alkyla-
tion leading to a depletion of mtDNA in intact cells
(Fig. 1). Here we report the synthesis and characterization
of a novel mitochondria-targeted alkylating ... supercoiled, linear and
relaxed-circular plasmid DNA, in order of increasing
apparent molecular weight (Fig. 3A) . Comparison of the
ethidium fluorescence (Fig. 3A) and t...
... protease core) had a C-terminal hexahistidine tag. The
catalytic domain was expressed as a GST-6XHis N-terminal fusion
protein. Variants designed for expression in mammalian cells had
an N-terminal ... require the interaction
with a rapidly titrated endogenous factor, rather than
a NLS [46]. Remarkably, the TRAF domain of USP7
also interacts with several nuclear proteins suc...