Báo cáo khoa học: Protein stabilization by compatible solutes Effect of diglycerol phosphate on the dynamics of Desulfovibrio gigas rubredoxin studied by NMR docx
... Protein stabilization by compatible solutes
Effect of diglycerol phosphate on the dynamics of
Desulfovibrio gigas
rubredoxin
studied by NMR
Pedro Lamosa
1
, David L. ... protein known to date [27]. In this context, assessing
the changes in the dynamic behaviour of proteins in the
presence of solutes is expected to shed light on the
stab...
... FEBS
Absorption spectra of the complexes of heme and
HOs resemble the spectrum of myoglobin with one
exception, the HO of Drosophila melanogaster. In the
HO of fruit fly, the iron of heme was not ... 3. Absorption spectra of various forms of the Syn HO-2–heme
complex. The concentration of the complex was 7 lm. The spectra
are the iron(III) (solid line), ir...
... characterization of the purified enzyme
The c oncentrated enzyme solution (fraction 6) was yellow i n
color. The enzyme s olution showed one main absorption
peak at 266 nm and a broad absorption band in the ... identified by GC-MS analysis, and the
determination of released ammonia. The coupled enzyme
assay revealed the mechanism of the deamination reaction
and the sub...
... at the end of the titration, even after
addition of excess Cu(I), some species still retained
Zn(II) ions.
The discussion of the Cu(I) coordination behavior
becomes more complex in view of the ... homonuclear Cu
5
preparations. Under these conditions, low Zn contents
Table 4. Analytical characterization of the recombinant preparations of the Cu complexes yielded by...
... tem-
perature.
Influence of interference
The influence of the main constituents of plasma on
the determination of 2.00 · 10
)6
molÆL
)1
GSH were
studied. The criterion for interference was fixed at a
± 5.0% variation ... bis(b-CD)s.
Because of the conformation of the linker of 1, the
nitrogen atoms and amido bond formed two chelate
rings to coordinate with copper (...
... models.
The first replaces the unigram component
of LDA topic models with multi-word se-
quences or collocations generated by an
AG. The second extension builds on the
first one to learn aspects of the ... the Doc and Doc
nonterminals — but it
has the advantage that the LDA distributions map
straight-forwardly onto PCFG nonterminals).
The terminals W of the CFG c...
... metabolism. The functional association was
further aided by integration of protein interaction
data with data on gene expression, localization, func-
tion, evolutionary conservation, protein structure ... than 70% of the
promoters are bound by at least one of the top 10%
most highly connected TFs. In addition, 82% of the
promoters are bound by at least one of th...
... (Milan Theater) and is not at the beginning
of that constituent. Finally, the syntactic depen-
dence trace of a word is the path of IOB values
from the root of the tree to the word itself. The
last ... measurement – the relative
position of the two words in their respective sen-
tences. The relative position is defined to be the
word’s absolute position divided...
... The assays were incubated
for 4 h and were stopped by the addition of 0.5
M
EDTA
pH 8 to a final concentration of 20 m
M
. The incorporated
14
C was measured by liquid scintillation counting of ... rubber
transferase activity at the final concentration of 10% (v/v)
in the rubber transferase assay, so these were the chosen
conditions for conducting the inhibitor studies...
... upper).
Higher concentrations of fatty acids accelerated the
formation of the LDs, but the toxic effects of fatty
acids on the cells became evident. Therefore, the cells
cultured under normal conditions ... [14], and detected the changes
in distribution of the labeled protein before and after
the formation of the LDs. To complete pulse-labeling
of the Halo-...