Báo cáo khoa học: Molecular cloning, expression analysis and functional confirmation of ecdysone receptor and ultraspiracle from the Colorado potato beetle Leptinotarsa decemlineata pdf
... 4114–4128 ª 2005 FEBS
Molecular cloning, expression analysis and functional
confirmation of ecdysone receptor and ultraspiracle from
the Colorado potato beetle Leptinotarsa decemlineata
Takehiko ... ecdysone receptor (EcR) and ultraspiracle (USP) of the
coleopteran Colorado potato beetle Leptinotarsa decemlineata (LdEcR and
LdUSP) w...
... rate of decrease of the linear form, the original data were fitted
by Y(t) ¼ e
–kt
· 100%, where Y is the percentage of the linear form, and t is the refolding time. For the rate of increase of the ... experiments.
For the rate of increase of the native form in the presence of cPDI, the original data were fitted by Y(t) ¼ Y
max
(1 ) e
–kt
) · 100%, where...
... PCR at either the 5¢-end or 3¢-end of the coding regions
of the two HRT cDNAs. The resulting fragments were
sequenced, digested with NcoIandBamHI, and ligated into
the NcoI–BamHI vector of pET32b(+), ... pET32b(+), yielding the
expression plasmids pETHRT1 and pETHRT2. Each of
the expression plasmids was used for transformation of
E. coli BL21(DE3), and 1 mL...
... region of the x-5 gliadin gene,
PCR primers were designed at the position of the initi-
ation and termination codons of the gene based on the
nucleotide sequences extracted from a database of wheat
expressed ... WDEIA.
We cloned and determined the nucleotide sequence
of two x-5 gliadin genes, x-5a and x-5b, from geno-
mic DNA of wheat cultivar Norin 61, a Jap...
... two exons (IA and IB) in the
X. laevis gene, with the site of the intron insertion localized
within the 5¢ UTR region of t he xMGP gene (Fig. 1 and
Table 2 ). The other introns (2, 3 and 4) are ... conserved sites within the MGP coding sequence
(Fig. 2 ). Analysis of the phase of each of the xMGP introns
located within the coding region revealed that intron...
... in the presence or
absence of the b
1B
subunit, whereas, under the same
conditions, the rate of inactivation was increased in the
presence of the b
1
subunit. The effects of b
1B
were further
assessed ... where G
max
is the maximum
conductance, z is the valence of the process and V
½
is the
midpoint voltage of activation.
Ionic current expression levels w...
... of the sinus
gland and the one which gives the name to the family
[3]. The main CHH activity is to elevate glucose concen-
tration in the hemolymph by a process of glycogen de-
gradation in the ... cysteine residues at the same positions as
that of other CHHs of penaeid shrimps (Fig. 1B).
Tissue-specific gene expression of L. schmitti
CHH gene
The size and ex...
... work, we
describe the cloning of the gene sequence of BmKIM, the
functional expression of the recombinant toxin in Escheri-
chia coli and its effect on the sodium channels of neurons
and ventricular ... obtained was the same as the
cDNA. Therefore, there is only one intron in the sequence
encoding the signal peptide. The position and structure of
BmKIM...
... organelle and membrane proteins are synthe-
sized and folded with the assistance of molecular chap-
erones and other folding factors in the endoplasmic
reticulum (ER). In many cases, the process of ... of the
53 kDa spot (5.3) was identical to a pI value calcu-
lated from the amino acid sequence of GmPDIL-3a,
and the isoelectric point of the 57 kDa spot (5....
... expression
were found in the promoter regions of both GmPDIL-1
and GmPDIL-2. However, the mRNA expression
patterns of GmPDIL-1 and GmPDIL-2 were different,
suggesting that the expression of these ... b-conglycinin during
the course of the folding process of these proteins.
Results
Cloning and expression of GmPDIL-1 and
GmPDIL-2
To clone the soybean orth...