Báo cáo khoa học: The effect of replacing the axial methionine ligand with a lysine residue in cytochrome c-550 from Paracoccus versutus assessed by X-ray crystallography and unfolding ppt
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The effect of replacing the axial methionine ligand with
a lysine residue in cytochrome c-550 from Paracoccus
versutus assessed by X-ray crystallography and unfolding
Jonathan A. R. Worrall, ... form, albeit
with the axial ligand still intact, with the main- and
side-chain atoms of K99 flipped up and away from the
heme...
... purification ensured that the
recombinant proteins have the same number of amino acids
as the native elongation factors.
Activity assays
The concentration of EF-Tu active in binding guanine
nucleotides, ... density is caused by the binding of a
deacylated tRNA to the ribosome rather than by a
change in the synthesis of (p)ppGpp. Deacylated tRNA
has a high co...
... of plasma alanine
aminotransferase (AlAT), aspartate aminotransferase
(AsAT) and LDH was assessed. The present study also
investigates the anti -in ammatory property of curcu-
min and capsaicin ... curcumin, capsaicin
and their combination on carrageenan induced
in ammation
To examine the postlocal anti -in ammatory potential of
the combination of spice principles curc...
... comparison of the indi-
vidually observed and predicted time values and for
comparison of within-group changes a repeated
measures analysis
of variance was used. If analysis of
variance showed an ...
patients had a normal left ventricular cavity size and
baseline ejection fraction as evaluated by radionuclide
angiography. Coronary artery disease was excluded
by...
... cellular retinaldehyde-binding protein. After 2 h of incuba-
tion at 37 °C in the dark, the generated retinoids were
extracted with 300 lL of methanol and 300 lL of hexane
and analyzed by normal-phase ... (A)
The lipid amount in each flotation fraction
was quantified by scintillation counting of
[
14
C]PC and expressed as a percentage of
the total amount of...
... communicated by the switchboard operators
through the hospital loudspeakers and paging system, and a
detailed log of all calls is maintained.
Criteria for medical emergency team activation
Calling ... this to aspects of nursing and medical
daily routine.
Materials and methods
The hospital
Austin Health is a university-affiliated teaching hospital with
three hospital...
... interactions of tau and Fyn may play
an important role in regulating the cellular localization
of Fyn and tau.
Results
Tau interacts with Fyn-SH2
Because tau contains tyrosines that could be targeted
by ... also an increase in the amount of
DRM-associated tau isolated from wild-type neurons
(Fig. 5A) . In contrast, pervanadate did not appear to
induce any change in...
... sites are
located in the concave face of the protein near regions
of invariant Trp and Asn arrays. The major binding
site spans ARM repeats 2, 3 and 4 (Fig. 1A) . In the
major binding site, the ... NLS in an extended conformation.
Database
Structural data are available in the protein Data Bank under the accession number
3OQS.
Structured digital abstract
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CLIC4...