Báo cáo khoa học: Enhanced peptide secretion by gene disrupt

Báo cáo khoa học: Enhanced peptide secretion by gene disruption of CYM1, a novel protease in Saccharomyces cerevisiae doc

... been investigated. Intracellular synthesis of CCK-22 was decreased in a cym1D0 strain accompanied by a n increased concentration of proCCK. In contrast, the fraction of extracellular CCK-22 was ... peroxidase and a dinamin-like GTPase, and is required for maintenance of mitochondrial morphology and DNA [45,46]. Whether disruption of CYM1 in uences the ability to mai...

Ngày tải lên : 07/03/2014, 16:20

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Tài liệu Báo cáo khoa học: "Enhanced word decomposition by calibrating the decision threshold of probabilistic models and using a model ensemble" pdf

... We 376 guarantee ∑ l ji A Pr(t ji |b ji )=1 with t ji being a transition from a certain l j,i−1 ∈ A B to l ji . The ad- vantage of the model is that instead of evaluating an exponential number of ... experience in terms of training set size. We want to remind the reader that our two algorithms are aimed at small datasets. We randomly split each dataset into 10 subsets wher...

Ngày tải lên : 20/02/2014, 04:20

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Tài liệu Báo cáo khoa học: Guanidinium chloride- and urea-induced unfolding of FprA, a mycobacterium NADPH-ferredoxin reductase Stabilization of an apo-protein by GdmCl docx

... Guanidinium chloride- and urea-induced unfolding of FprA, a mycobacterium NADPH-ferredoxin reductase Stabilization of an apo-protein by GdmCl Nidhi Shukla 1 , Anant Narayan Bhatt 1 , Alessandro ... Changes in functional and structural properties of FprA on incubation with increasing concentration of GdmCl at pH 7.0 and 25 °C. (A) Changes in enzymatic activity of FprA on incu...

Ngày tải lên : 19/02/2014, 16:20

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Báo cáo khoa học: Abortive translation caused by peptidyl-tRNA drop-off at NGG codons in the early coding region of mRNA potx

... the A B Fig. 1 Plasmid constructs are derivatives of pDA3480 [9,10]. The 5¢ end of the transcript is 5¢-AAUUGUGAGCGGAUAACAAUUUCA- CCAGGUAAUAAAUU AAAUAAAAUUUAAAUAUG-3¢ for the gene variants that ... [15–18]. Drop-off at a pair of rare AGA AGG codons has been demonstrated in a natural gene and ribosome stalling and drop-off at a pair of argin- ine codons in mini-genes [1...

Ngày tải lên : 30/03/2014, 20:20

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Báo cáo khoa học: Interaction between catalytically inactive calpain and calpastatin Evidence for its occurrence in stimulated cells docx

... digest- ible protein substrate, and finally calpastatin. The finding that calpastatin was the most efficient ligand at promoting calpain transition, detected as an increase in mAb that bound to calpain, is ... of arachidonate suggests that conditions promoting calpain–calpastatin interaction in the cytosol can be Ca 2+ independent and precede calpain activation. Additional information wa...

Ngày tải lên : 07/03/2014, 12:20

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Báo cáo khoa học: Adenylyl cyclase Rv0386 from Mycobacterium tuberculosis H37Rv uses a novel mode for substrate selection ppt

... catalytic domain fused to an AAA-ATPase and a helix-turn-helix DNA-binding domain. In Rv0386, the standard substrate, adenine-defining lysine-aspartate couple is replaced by glutamine-asparagine. The ... has a glutamine- asparagine pair at the positions defining ATP as a substrate instead of the lysine-aspartate consensus. We show that the purified catalytic domain of Rv0386 is a...

Ngày tải lên : 07/03/2014, 21:20

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Báo cáo khoa học: Contributions to catalysis and potential interactions of the three catalytic domains in a contiguous trimeric creatine kinase doc

... in adjacent active sites, essentially allowing only one of a set of interacting active sites to complete a catalytic cycle at a time. Further understanding of catalysis and the interaction of active ... three catalytic domains in a single polypeptide. Proc Natl Acad Sci USA 101, 16555–16560. 24 Kinukawa M, Nomura M & Vacquier VD (2007) A sea urchin sperm ﬂagellar ade...

Ngày tải lên : 16/03/2014, 06:20

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Báo cáo khoa học: Isolation, characterization, sequencing and crystal structure of charybdin, a type 1 ribosome-inactivating protein from Charybdis maritima agg. potx

... Bull 50, 747–750. 2 Narayanan S, Surolia A & Karande AA (2004) Ribo- some-inactivating protein and apoptosis: abrin causes cell death via mitochondrial pathway in Jurkat cells. Biochem J 377, ... the puriﬁcation, characterization and structural determination of charybdin, a novel 29-kDa protein from bulbs of C. maritima agg. Charybdin was characterized by biochemical metho...

Ngày tải lên : 16/03/2014, 14:20

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Báo cáo khoa học: R120G aB-crystallin promotes the unfolding of reduced a-lactalbumin and is inherently unstable ppt

... position in aA-crystallin, R116C, caused congenital cataract in humans [12]. In aA-crystallin and aB-crystallin, R116 and R120, respectively, are located within the con- served a- crystallin domain. ... resultant data were transformed to a mass scale using an algorithm in the masslynx software (Waters ⁄ Micromass). Chaperone activity of R120G aB-crystallin Reduction assay Bovine...

Ngày tải lên : 16/03/2014, 18:20

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Báo cáo khoa học: Cdk2 activity is dispensable for triggering replicon initiation after transient hypoxia in T24 cells docx

... reoxygenation caused, within 30 min, a signiﬁcant decrease of chromatin associated Cdc6 accompanied by a phosphoryla- tion of a part of it. The decrease of chromatin associated Cdc6 as well as the ... approa- ches. Microinjection of antibodies against Cdk2, cyclin E or cyclin A and the antisense mRNA of Cdk2 block initiation of DNA synthesis in mammalian cells [19,...

Ngày tải lên : 16/03/2014, 23:20

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