... 5¢-aataatccatggggagtaatacttcctggcgta
aaagtgaagtcc-3¢ and 5¢-attattggatccctattgctggtccgattctgccag-3¢.
3-TPR NlpI primers (residues 62–197) were 5¢-aataatccatgg
gggcacagcttttatatgagcgcggag-3¢ and 5¢-aataatggatcctcactgttc
cttatccgatttttcgaagtgc-3¢. ... interactions
TPRs
Many features of the distribution of side chain con-
tacts within NlpI are typical of a TPR protein. The
side...
... picolinic
acid (PA), quinolinic acid (QA) and NAD homeostasis. Indeed, the enzyme
stands at a branch point of the tryptophan to NAD pathway, and deter-
mines the final fate of the amino acid, i.e. transformation ... in the crys-
tal lattice by applying the crystallographic two -fold
axis. PfACMSD was reported to be a dimer, with
subunits related by a dyad axis in the...
... 5¢-AGATCTTATCACTCCCTTACTAAGTCGATG-3¢
for the b subunit and 5¢-CATATGAGCGAGAAAACCA
TGCGCGTGCAG-3¢ and 5¢-AGATCTTAGCTTCCTTT
ACGCAGCTTATGC-3¢ for the c subunit. The segments
were digested with NdeIandBglII ... Poznanskaya, A. A., Yakusheva, M.I. & Yakovlev, V .A. (1977)
Study of the mechanism of action of adenosylcobalamin-depen-
dent glycerol dehydratase from Aerobacter aer...
... annexin Anx24(Ca32), but significant differences
are seen when compared to the structure of nonplant
annexins. A comparison with the structure of the mamma-
lian annexin AnxA5 indicates that canonical ... relatives.
Calcium binding has been identified as a landmark
feature of animal, plant, and metazoan annexin proteins. As
structurally established for annexin A5 [16], th...
... at both ends. In the case of
Xgh7 4A, the active cleft is open. Although a precise anal-
ysis of the mode of action of Xgh7 4A has not been
performed, Xgh7 4A appears to be an endoglucanase
because ... residues in the backbone chain.
Typically, treatment of an XXXG-type xyloglucan
with these endoglucanases generates oligosaccharides
with a tetrasaccharide backbone...
... Ciclosporin A inhibits PPWD1 activity against suc-AAPF-pNA. (C) Binding and
catalysis of isomerization for the standard substrate suc-AAPF-pNA. Black and red spectra of the Ala residues in the peptide are ... biophysical assays. Using a colorimetric
coupled assay against succinyl-Ala-Ala-Pro-Phe-p-nit-
roaniline (suc-AAPF-pNA), a well-characterized CypA
substrate, we found tha...
... and catalytic domains of the a subunit.
In the heterohexamer, the Rieske domain interacts
with the base of the adjacent b subunit and the cata-
lytic domain of the adjacent a subunit. Most of the ... vicinity of
the catalytic iron, aca11 contains residues 356–360 and
carries the totally conserved amino acids Gly
a
354,
Glu
a
357, Asp
a
359 and Asn
a...
... using partial hydrolyates of pullulan and
an inactive TVA II mutant, D325N. A hexasaccharide
containing two panose units, 4
3
-a- panosylpanose (4
3
-P2)
(Fig. 2A) , was thereby prepared. We analyzed ... of Kunkel et al. [17]. To construct the Y37 4A
mutant, the following oligonucleotide was used as a
mutagenic primer: 5¢-GATCACACTCTCGCG
AAACA
AATAATTCATCACCG-3¢. The underline...
... by the
similarity of the quaternary structures of KlPDC and
pyruvamide-activated ScPDC, although the first has
been crystallized in the absence of any allosteric effec-
tors and the latter in the ... described the structural consequences
of substrate activation on the basis of the crystal struc-
ture of pyruvamide-activated ScPDC compared to that
of ScPDC crys...