Báo cáo khoa học: The crystal structure of NlpI A prokaryotic tetratricopeptide repeat protein with a globular fold potx

Báo cáo khoa học: The crystal structure of NlpI A prokaryotic tetratricopeptide repeat protein with a globular fold potx

Báo cáo khoa học: The crystal structure of NlpI A prokaryotic tetratricopeptide repeat protein with a globular fold potx

... 5¢-aataatccatggggagtaatacttcctggcgta aaagtgaagtcc-3¢ and 5¢-attattggatccctattgctggtccgattctgccag-3¢. 3-TPR NlpI primers (residues 62–197) were 5¢-aataatccatgg gggcacagcttttatatgagcgcggag-3¢ and 5¢-aataatggatcctcactgttc cttatccgatttttcgaagtgc-3¢. ... interactions TPRs Many features of the distribution of side chain con- tacts within NlpI are typical of a TPR protein. The side...
Ngày tải lên : 07/03/2014, 16:20
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Tài liệu Báo cáo khoa học: The crystal structure of human a-amino-b-carboxymuconatee-semialdehyde decarboxylase in complex with 1,3-dihydroxyacetonephosphate suggests a regulatory link between NAD synthesis and glycolysis ppt

Tài liệu Báo cáo khoa học: The crystal structure of human a-amino-b-carboxymuconatee-semialdehyde decarboxylase in complex with 1,3-dihydroxyacetonephosphate suggests a regulatory link between NAD synthesis and glycolysis ppt

... picolinic acid (PA), quinolinic acid (QA) and NAD homeostasis. Indeed, the enzyme stands at a branch point of the tryptophan to NAD pathway, and deter- mines the final fate of the amino acid, i.e. transformation ... in the crys- tal lattice by applying the crystallographic two -fold axis. PfACMSD was reported to be a dimer, with subunits related by a dyad axis in the...
Ngày tải lên : 18/02/2014, 06:20
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Tài liệu Báo cáo khoa học: The crystal structure of coenzyme B12-dependent glycerol dehydratase in complex with cobalamin and propane-1,2-diol pptx

Tài liệu Báo cáo khoa học: The crystal structure of coenzyme B12-dependent glycerol dehydratase in complex with cobalamin and propane-1,2-diol pptx

... 5¢-AGATCTTATCACTCCCTTACTAAGTCGATG-3¢ for the b subunit and 5¢-CATATGAGCGAGAAAACCA TGCGCGTGCAG-3¢ and 5¢-AGATCTTAGCTTCCTTT ACGCAGCTTATGC-3¢ for the c subunit. The segments were digested with NdeIandBglII ... Poznanskaya, A. A., Yakusheva, M.I. & Yakovlev, V .A. (1977) Study of the mechanism of action of adenosylcobalamin-depen- dent glycerol dehydratase from Aerobacter aer...
Ngày tải lên : 21/02/2014, 03:20
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Báo cáo khoa học: The crystal structure of annexin Gh1 from Gossypium hirsutum reveals an unusual S3 cluster Implications for cellulose synthase complex formation and oxidative stress response potx

Báo cáo khoa học: The crystal structure of annexin Gh1 from Gossypium hirsutum reveals an unusual S3 cluster Implications for cellulose synthase complex formation and oxidative stress response potx

... annexin Anx24(Ca32), but significant differences are seen when compared to the structure of nonplant annexins. A comparison with the structure of the mamma- lian annexin AnxA5 indicates that canonical ... relatives. Calcium binding has been identified as a landmark feature of animal, plant, and metazoan annexin proteins. As structurally established for annexin A5 [16], th...
Ngày tải lên : 17/03/2014, 03:20
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Báo cáo khoa học: The crystal structure of a xyloglucan-specific endo-b-1,4glucanase from Geotrichum sp. M128 xyloglucanase reveals a key amino acid residue for substrate specificity potx

Báo cáo khoa học: The crystal structure of a xyloglucan-specific endo-b-1,4glucanase from Geotrichum sp. M128 xyloglucanase reveals a key amino acid residue for substrate specificity potx

... at both ends. In the case of Xgh7 4A, the active cleft is open. Although a precise anal- ysis of the mode of action of Xgh7 4A has not been performed, Xgh7 4A appears to be an endoglucanase because ... residues in the backbone chain. Typically, treatment of an XXXG-type xyloglucan with these endoglucanases generates oligosaccharides with a tetrasaccharide backbone...
Ngày tải lên : 23/03/2014, 05:22
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Báo cáo khoa học: The crystal structure of human WD40 repeat-containing peptidylprolyl isomerase (PPWD1) pdf

Báo cáo khoa học: The crystal structure of human WD40 repeat-containing peptidylprolyl isomerase (PPWD1) pdf

... Ciclosporin A inhibits PPWD1 activity against suc-AAPF-pNA. (C) Binding and catalysis of isomerization for the standard substrate suc-AAPF-pNA. Black and red spectra of the Ala residues in the peptide are ... biophysical assays. Using a colorimetric coupled assay against succinyl-Ala-Ala-Pro-Phe-p-nit- roaniline (suc-AAPF-pNA), a well-characterized CypA substrate, we found tha...
Ngày tải lên : 23/03/2014, 07:20
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Báo cáo khoa học: The crystal structure of the ring-hydroxylating dioxygenase from Sphingomonas CHY-1 pot

Báo cáo khoa học: The crystal structure of the ring-hydroxylating dioxygenase from Sphingomonas CHY-1 pot

... and catalytic domains of the a subunit. In the heterohexamer, the Rieske domain interacts with the base of the adjacent b subunit and the cata- lytic domain of the adjacent a subunit. Most of the ... vicinity of the catalytic iron, aca11 contains residues 356–360 and carries the totally conserved amino acids Gly a 354, Glu a 357, Asp a 359 and Asn a...
Ngày tải lên : 23/03/2014, 09:21
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Báo cáo khoa học: The crystal structure of a hyperthermostable subfamily II isocitrate dehydrogenase from Thermotoga maritima pdf

Báo cáo khoa học: The crystal structure of a hyperthermostable subfamily II isocitrate dehydrogenase from Thermotoga maritima pdf

... [17]. Table 3. Primer sequences. TmIDH mutant Primer sequence R186M 5’-CCTGGAGAAATCGATCA TGAGCTTCGCTCAGTCGTG-3’ 5’-CACGACTGAGCGAAGCT CATGATCGATTTCTCCAGG-3’ F205M 5’-AAAAGGTCGACATCTGG ATGGCGACGAAAGACACGATC-3’ 5’-GATCGTGTCTTTCGTCGC CATCCAGATGTCGACCTTTT-3’ D36N ... 5’-CACTCTCGAAGAGTTCATA AACGAAGTGAAGAAGAATCTC-3’ 5’-GAGATTCTTCTTCACTTCGT TTATGAACTCTTCGAGAGTG-3’ M. Karlstro ¨ m et al. Structure a...
Ngày tải lên : 23/03/2014, 10:21
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Báo cáo khoa học: The crystal structure of Thermoactinomyces vulgaris R-47 a-amylase II (TVA II) complexed with transglycosylated product potx

Báo cáo khoa học: The crystal structure of Thermoactinomyces vulgaris R-47 a-amylase II (TVA II) complexed with transglycosylated product potx

... using partial hydrolyates of pullulan and an inactive TVA II mutant, D325N. A hexasaccharide containing two panose units, 4 3 -a- panosylpanose (4 3 -P2) (Fig. 2A) , was thereby prepared. We analyzed ... of Kunkel et al. [17]. To construct the Y37 4A mutant, the following oligonucleotide was used as a mutagenic primer: 5¢-GATCACACTCTCGCG AAACA AATAATTCATCACCG-3¢. The underline...
Ngày tải lên : 23/03/2014, 12:20
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Báo cáo khoa học: The crystal structure of pyruvate decarboxylase from Kluyveromyces lactis Implications for the substrate activation mechanism of this enzyme ppt

Báo cáo khoa học: The crystal structure of pyruvate decarboxylase from Kluyveromyces lactis Implications for the substrate activation mechanism of this enzyme ppt

... by the similarity of the quaternary structures of KlPDC and pyruvamide-activated ScPDC, although the first has been crystallized in the absence of any allosteric effec- tors and the latter in the ... described the structural consequences of substrate activation on the basis of the crystal struc- ture of pyruvamide-activated ScPDC compared to that of ScPDC crys...
Ngày tải lên : 30/03/2014, 10:20
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