Báo cáo khoa học: The role of helix 8 and of the cytosolic C-termini in the internalization and signal transduction of B1 and B2 bradykinin receptors potx
... in obese mice
In order to gain insights into the potential biologically
relevant role of miR-27 in the regulation of adipose tis-
sue functions in vivo, we examined the expression of
miR-27 in ... differentiation
was initiated at 48 h post-transfection by maintaining the cells in culture medium containing 2% horse serum. Cells were fixed on day 4 of
differentiati...
... mechanism of antitumour
action of BS-RNase is based on the ability of the protein
to resist the neutralizing action of the cytosolic RNase
inhibitor, a resistance due to the dimeric structure of the
enzyme. ... Effects of protein RNase inhibitor and
substrate on the quaternary structures of bovine seminal RNase.
Biochemistry 35, 388 0– 388 5.
6. Leland,P.A.,Schu...
... molecule
comprising Ôresidues in the protruding domain consisting
of the type VI b-turn and the first part of b-strand 3Õ (i.e. the
b-hairpin loop and part of the adjoining b-sheet) and
Ôresidues in the loop ... obtained by other groups
who also point to the role of the connecting loop of the
b-hairpin of defensins. A combination of mutational
analys...
... deviates from the original position. In the struc-
tural deviation, the solvent ASA of the side-chain of His210
increased with the decrease in size of the side-chain at
residue 169 (Table 1 and Fig. ... effect of the size of the residue at position 169 may be
related to the solvent accessibility of the side-chain of
His210 (Fig. 3C). When the accessib...
... hydrolysis, resulting in an
increase in intracellular sterol trafficking. It is unclear, then,
whether the corresponding increase in pregnenolone pro-
duction results from an increase in free sterol ... pregnenolone, including the
effect of the steroid products on the yeast sterol synthesis
pathway, acetylation of the product, availability of the
substrate, electron fl...
... loose
binding of azide to the trinuclear copper center. As the
hydrogen bond between the C-terminus and His140 is
broken, and the stability of the trinuclear site is
decreased, the binding of azide ... preventing binding of oxygen.
We analyzed the azide inhibition and determined the
inhibition constants for sodium azide, in order to see
the effect of the...
... signaling [23]. Domains similar to
the ATM-binding domain of Nbs1 are also found in
ATRIP and Ku80 and are required for the interaction
of these proteins with the ATM-related kinase, ATR,
and the ... activation of the S-phase checkpoint. The Mre11-bind-
ing domain is responsible for binding to Mre11 during formation of
the Mre11–Rad50–Nbs1 (MRN) complex. The AT...
... release restores the inward-
facing state. The linker region of P-gp can be assumed
to connect the C-terminal helix of subunit A (shown in
red in Fig. 7) with the N-terminal elbow helix of sub-
unit ... containing
P-gp, the results could be affected by other proteins.
In fact, it was reported that the linker region of P-gp
interacts with other proteins such as RI...
... sequences in the N-terminal region of the fibrin Bb-chain, have been
investigated. Peptides Bb12–26 and Bb26–46, but not Bb40–46, Bb19–26,
and Bb26–36, proved to be specific inhibitors of fibrin polymerization. ... activity of synthetic peptides mimick-
ing the various fragments of the N-terminal region of
the fibrin Bb-chain was investigated. Two peptides,
Bb12–26 and...
... in the target recombinant protein
[22]. The distribution of side chains in the sequence,
such as occurs in protein folding, plays a pivotal role
in determining the conformational properties of ... problems in the
development of the biotechnology industry. Over the last decade, the fine
dissection of the quality control system in bacteria and the reco...