Báo cáo khoa học: The Y42H mutation in medium-chain acyl-CoA dehydrogenase, which is prevalent in babies identified by MS/MS-based newborn screening, is temperature sensitiv pptx
... 2004 The MCAD Y42H mutation is temperature sensitive (Eur. J. Biochem. 271) 4063
The Y42H mutation in medium-chain acyl-CoA dehydrogenase,
which is prevalent in babies identified by MS/MS-based newborn
screening, ... observed,
indicating that the temperature- induced change in the fold
of the structure is indistinguishable between the th...
... the peak
of the emission spectrum at 342 nm. The concentration
of Na
+
was increased by aspirating a defined volume of
thrombin solution dissolved in the cuvette in the above buf-
fer containing ... of the reduced A-chain in the mutant enzyme. The sodium ion bind-
ing affinity was not significantly perturbed by Lys9 deletion, although the
linked increase in intrinsi...
... PKCf in signal
transduction. Our results indicate that PKCf is not involved
in the activation of glycogen synthesis by insulin. This
finding is in good agreement with a previous report showing
that ... taken and the experiment
was started by the addition of agonists to the dishes.
Inhibitors were added 10 min before the agonists. The
incubation was terminated by rap...
... are classified into
three groups, the neutral subtilisins, the alkaline subtilisins
and the ALP I-typeÕ subtilisin (Fig. 1) [3]. The neutral
subtilisins consist of the subtilisins from neutrophilic
Bacillus ... on the molecular surface. In the wild-
type enzyme, the side chain of Asp266 interacted with the
amino group of the main chain at Glu268. The oxygen of
the main...
... V-ATPase is required
further downstream in the secretory pathway but it is not
known in which compartment the V-ATPase becomes
active and which mechanism is involved in the targeting of
the V-ATPase ... completed in t his c ompartment by the build-up of the V
1
onto the V
0
[28,29]. Given the pH in the ER, the V-ATPase
should be considered as being es...
... conserved kinase
domain [7]. In tomato, PTI1 can physically interact
with the serine ⁄ threonine kinase PTO, which confers
resistance to the bacterial pathogen P. syringae pv
tomato carrying the avirulence ... & Martin GB (1995) The
tomato gene Pti1 encodes a serine ⁄ threonine kinase that
is phosphorylated by Pto and is involved in the hyper-
sensitive response. Cel...
... harbours an iscR::lacZ fusion [42].
IscR is a Fe ⁄ S protein encoded by the first gene of the
isc operon, and it is involved in the transcriptional
regulation of both the isc and the suf operons. In its
holoform, ... bodies.
Because the histidine-tag can in uence the solubility
and the folding of the protein in some cases, we also
purified the OsiS protein witho...
... microbody-localized
APXs of higher plants [8]. This finding raised the
hypothesis that a region within the C-terminal half of
the chloroplastic APXs is involved in their susceptibil-
ity to hydrogen peroxide.
Results
Preparation ... isoforms in chloroplasts, one of
which is soluble in the stroma and the other of which
is bound to the stromal side of thylakoid membra...
... to the pathogenesis of atherosclerosis.
In the vascular system, the major source of reactive oxygen species is the
NADPH oxidase (Nox) family. Nox1 is induced by lipopolysaccharide
(LPS) in macrophages, ... supported by fluores-
cent microscopy. In untreated cells, b-catenin was
mainly localized in the cytosol at a low level. After
45 min of treatment with LPS, the...
... concentrations of KCl. The other conditions were
as described above. In all cases, K
+
leads t o a reduction of ouabain
binding. (B) By plottin g the reciprocal binding of [
3
H]ouabain against
the K
+
concentration, ... explained on t he basis of structural
disturbances leading to the loss of a/b subunit interactions.
Together with the results of previous investigations, show-...