Báo cáo khoa học: Expression and characterization of the pro

Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx

... Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family Ste ´ phane Canaan 1 , Damien Maurin 1 , ... is composed of 7 parallel b strands associated to an anti- parallel strand (b2) and is surrounded by 5 helices (a1 , a2 , a3 , a7 anda8). T he second...

Ngày tải lên : 07/03/2014, 16:20

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Báo cáo khoa học: Expression and characterization of the bioﬁlm-related and carnosine-hydrolyzing aminoacylhistidine dipeptidase from Vibrio alginolyticus pot

... binding ACTATTGATGAA GCCGCGGGCATGACAGGTGC D17 3A Zinc binding CCTTCTAAATACA GCTAGCGAACAAGAAGGCG H46 1A Zinc binding CCAACCATCAAGTTCCCT GCTAGCCCAGATGAG Characterization of V. alginolyticus PepD T Y. Wang ... CGCTCGGGGCA GCTAACGGCATCGGCATGGC D119X Zinc binding CGCTCGGGGCA NNNAACGGCATCGGCATGGC E14 9A General base CTGACGATCGAT GCAGAAGCAGGCATGACAGG E149X General base CTGACAATTGAT NNNGAAGCAG...

Ngày tải lên : 23/03/2014, 06:20

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Báo cáo khoa học: Expression and characterization of recombinant 2¢,5¢-oligoadenylate synthetase from the marine sponge Geodia cydonium ppt

... discovered as a part of the interferon antiviral pathway in mammals [1,2]. In higher animals (vertebrates), when activated by dsRNA, 2- 5A synthetases catalyze the polymerization of ATP into unusual 2¢,5¢-linked ... optimal for the nuclease (high NaCl and phosphate concentrations and the absence of Mg 2+ ). Another nuclease treatment was carried out after puriﬁcation and...

Ngày tải lên : 23/03/2014, 09:20

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Báo cáo khoa học: Expression and characterization of cyanobacterium heme oxygenase, a key enzyme in the phycobilin synthesis Properties of the heme complex of recombinant active enzyme potx

... Fig. 7, panel A. Addition of ascorbate to the heme–Syn HO-1 complex commences the reaction, which is monitored by the steady decrease of the Soret and 498 nm bands and the shift of the band at 630 ... gauss is attributable to the 14 N nuclei of the axial ligand trans to the nitrosyl ligand. This ﬁrmly establishes that the proximal ligand of the heme...

Ngày tải lên : 23/03/2014, 20:22

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Báo cáo khoa học: Expression and characterization of soluble forms of the extracellular domains of the b, c and e subunits of the human muscle acetylcholine receptor pot

... Psaridi-Linardaki L, Trakas N, Mamalaki A & Tzartos SJ (2005) Speciﬁc immunoadsorption of the autoantibo- dies from myasthenic patients using the extracellular domain of the human muscle acetylcholine ... Tzartos SJ, Barkas T, Cung MT, Mamalaki A, Mar- raud M, Orlewski P, Papanastasiou D, Sakarellos C, Sakarellos-Daitsiotis M, Tsantili P, et al. (1998) Anat- omy of the...

Ngày tải lên : 30/03/2014, 10:20

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Báo cáo khoa học: Cloning and characterization of the genes encoding toxic lectins in mistletoe (Viscum album L) pot

... and XhoI ML2p A- chain 5¢- GATATA CATATG TACGAGCGTCTTCGTCTTCGTGTTACGCATC -3¢ 5¢- CACAC CTCGAG TTATTAAGAAGAAGACGGACGGTCCCGGCATAC -3¢ NdeI and XhoI ML3p A- chain 5¢- GATATA CATATG TACCGTCGTATTAGCCTTCGTGTCACGGAT -3¢ 5¢- CACAC GAATTC TTATTAAGAAGAAGAAGAACGGTCCCTGCATAC -3¢ NdeI and EcoRI ML3.1p A- chain 5¢- GATATA CATATG TACGAGCGTCTTCGTCTTCGTGTTACGCATC -3¢ 5¢- CACAC GAATTC TTATTAAGAAGAAGAA...

Ngày tải lên : 07/03/2014, 15:20

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Báo cáo khóa học: Isolation and characterization of the Xenopus HIVEP gene family ppt

... underlined: wt, 5¢-AGAGAGAA TGAGAGGCTTCCCAATAGC-3¢;mut1,5¢-AGAGAG AATGA TAGGCTTCACAATAGC-3¢;mut2,5¢-AGAG AGAATGA TAGGCTTCCCAATAGC-3¢;mut3,5¢-AGA GAGAATGAGAGGCTTC ACAATAGC-3¢. Binding reactions were ... derived from the additional sequence obtained in the ﬁrst ampliﬁcation (5¢-GARAAYTTYGARAAYCAYAARAARTTYTAYTG-3¢ and 5¢-AGTTCTAATGCTATGTTTGGATGC-3¢)affor- ded a product of 1.7 kb. Add...

Ngày tải lên : 30/03/2014, 13:20

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Báo cáo khoa học: Fatty acid regulation of adenylyl cyclase Rv2212 from Mycobacterium tuberculosis H37Rv doc

... active state (pH 6), the catalytic domains align as a closed dimer capable of binding ATP and of catalysis. In the in- active state (pH 8), the catalytic domains are drawn apart by extended a- helices ... mycobacterial class IIIb ACs, the HAMP domains appear to directly act as modulators of AC activity, possibly transmitting signals that may be picked up by a receptor f...

Ngày tải lên : 30/03/2014, 10:20

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Tài liệu Báo cáo khoa học: Expression and secretion of interleukin-1b, tumour necrosis factor-a and interleukin-10 by hypoxia- and serum-deprivation-stimulated mesenchymal stem cells Implications for their paracrine roles ppt

... cardiac fibrosis in a paracrine manner under ischaemic conditions. Taken together, these findings may improve understanding of the cellu- lar and molecular basis of the anti-inflammatory and paracrine ... transcription and secretion of IL-10 Because of the lack of secretion of the inflammatory cytokines IL-1b and TNF -a from hypoxia ⁄ SD-stimulated MSCs, as well...

Ngày tải lên : 18/02/2014, 04:20

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Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx

... in the Caryophyllaceae, Sambucaceae, Cucurbitaceae, Euphorbiaceae, Phytolaccaceae and Poaceae [1]. Although many are potentially useful in agriculture and medicine because of their antiviral properties ... displayed hemagglutination and toxicity toward mice (data not shown), additional efforts to cleanly isolate the isoform were not successful and further characterization wa...

Ngày tải lên : 18/02/2014, 16:20

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