Báo cáo khoa học: Puriﬁcation and structural analysis of the

Báo cáo khoa học: Puriﬁcation and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot

... Hordeum A. A.N N-QRIN LQ GG IFW AGAD ASDA.NS.VS D. D 113 Triticum G .A. S N-QRIN LQ GG IFW AGAD AADA.NA.VG D. D 113 Zea AYA.S A- QRQG LI GG FW AGAD.SASDA.GS.VS QY.DHDT.S. 112 Nicotiana AYA.N S-Q.AA ... GAG TCA GCC TCC CTC ACA ACA CCA 216 73TTREEQPAAEAAASTAGGSQ ACC ACC AGG GAA GAA CAA CCG GCG GCA GAG GCC GCG GCG TCC ACC GCT GGC GGT AGC CAA 276 93QEGYGSTQLPSDEPLNGLND CAA GAA GGA TAT GGC...

Ngày tải lên : 07/03/2014, 12:20

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Báo cáo khoa học: Puriﬁcation and structural study of the b form of human cAMP-dependent protein kinase inhibitor pdf

... with oligonucleotides 5¢-CCCCATATGATGAGGACAGATT CATCAAAAATG-3¢ and 5¢-CATGGATCCTCATTTT TCTTCATTTTGAGGC-3¢. The ampliﬁed PCR fragment was inserted into plasmid vector pET between the endo- nuclease sites NdeIandHindIII. ... signal ATTAAA (1018– 1023) and poly (A) tail (Fig. 1). The human PKIb protein predicted by the open reading frame is 78 amino acids in length with a calcula...

Ngày tải lên : 07/03/2014, 15:20

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Báo cáo khoa học: Puriﬁcation and kinetic analysis of the two recombinant arogenate dehydrogenase isoforms of Arabidopsis thaliana pptx

... of the two A. thaliana arogenate dehydrogenases TyrAAT1 and TyrAAT2 deduced from their respective coding sequences (A) and alignment of amino acid sequence of the two protein domains of TyrAAT1 ... synthase and shiki- mate kinase, and not less than six for arogenate dehydratase. Analysis of the patterns of expression of the two A. thaliana arogenate dehyd...

Ngày tải lên : 17/03/2014, 11:20

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Tài liệu Báo cáo khoa học: Puriﬁcation and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx

... two additional residues, Leu and Val, at the C-terminus, which are cleaved during maturation. Fig. 2. cDNA and the deduced precursor sequence of conomarphin. The signal peptide is shadowed and ... shadowed and the mature peptide is underlined. The polyA signal AATAAA in the 3¢-UTR is also underlined. The cDNA of conomarphin has been deposited in the Gen- bank d...

Ngày tải lên : 18/02/2014, 17:20

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Tài liệu Báo cáo khoa học: Puriﬁcation and cDNA cloning of a cellulase from abalone Haliotis discus hannai ppt

... nucleotide and amino acid are indicated in the right of each row. The translational start codon ATG, termination codon TAA, and a putative polyadenylation signal AATAAA are boxed. A putative signal ... Relative positions of Hd1-DNA, Hd2-DNA, Hd5RACE-DNA, Hd3RACE-DNA, and HdFull-DNA are indicated as solid lines. Bold lines in both sides of the cDNAsindicateprimersusedfort...

Ngày tải lên : 20/02/2014, 23:20

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Báo cáo khoa học: Identiﬁcation and structural characterization of a sialylated lacto-N-neotetraose structure in the lipopolysaccharide of Haemophilus inﬂuenzae pptx

... residue are indicated. (B) The assignments of the 1 H-resonances of the GlcNAc and Gal II residues are indicated. The spectrum was recorded in D 2 O at pH 7.0 and 295 K. 4016 A. D. Cox et al. ... acceptor the sialylated tetrasaccharide unit cannot be attached. In strain RM118, and the RM118 lgtC mutant strain, we have now identiﬁed two different sialylated species, n...

Ngày tải lên : 23/03/2014, 21:21

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Báo cáo khoa học: Puriﬁcation and functional characterization of human 11b hydroxylase expressed in Escherichia coli doc

... PCR using the 5¢-primer (CGCCATATGGCTACTAAAGCTG CTCGTGTTCCACGTACAGTGCTGCCA) and 3¢-primer (GCGAAGCTTAATGATGATGATGATGATGGTTGAT GGCTCTGAAGGTGAGGAG) and inserted into the NdeI ⁄ HindIII-digested pET17b ... expression of AdR and Adx. The plasmid containing the coding sequence for AdR was kindly provided by Y. Sagara [32]. Recombinant Adx and AdR were puriﬁed as described previou...

Ngày tải lên : 30/03/2014, 04:20

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Báo cáo khóa học: Puriﬁcation and functional characterization of insecticidal sphingomyelinase C produced by Bacillus cereus ppt

... addition, the N-terminal and internal amino-acid sequences deduced from the cloned gene were the same as those determined by Edman degradation. The recombinant protein expressed by E. coli was puriﬁed ... degradation (data not shown). Another insecticidal protein was also puriﬁed from fraction ii. This protein migrated as a single band at a molecular mass of 34 kDa...

Ngày tải lên : 30/03/2014, 13:20

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Báo cáo khoa học: Puriﬁcation and partial characterization of seven glutathione S -transferase isoforms from the clam Ruditapes decussatus pptx

... collected from the lagoon of Thau (N.W. Mediterranean sea) and placed on ice for transport to the laboratory. They were then transferred to a 200-L aquarium ﬁlled with natural aerated sea water, in a ... cross-reacted with antisera to pi and alpha classes and the isoform 5-5 cross-reacted with the antisera to mu and pi classes. Subunit 4 was recognized by the three a...

Ngày tải lên : 31/03/2014, 09:20

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Báo cáo khoa học: Identiﬁcation and expression analysis of an IL-18 homologue and its alternatively spliced form in rainbow trout (Oncorhynchus mykiss) doc

... partial deletion of exon 2. However, this deletion did not disrupt protein translation and thus the alternative spliced mRNA remained in-frame and potentially translated into a 182 amino acid ... cytokine. Materials and methods Cloning and sequencing of genomic DNA and cDNA All products ampliﬁed by PCR were ligated into the pGEM-Teasy vector (Promega) and transformed i...

Ngày tải lên : 16/03/2014, 16:20

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