Báo cáo khoa học: Structure of the complex of a yeast glucoamylase with acarbose reveals the presence of a raw starch binding site on the catalytic domain doc
... GLU H44 7A forward (5¢- GCAAGTCATTT
TGGATGCTATTAATGATGATGGCTC-3¢), GLU H44 7A
reverse (5¢- GAGCCATCATCATTAATAGCATCCAAAA
TGACTTGC-3¢); GLU T46 2A forward (5¢- GAACAACTT
AACAGATATGCCGGTTATT CCACCGGT ... similar to that
of the catalytic domain of A. awamori and T. thermos-
accharolyticum glucoamylases, with the active site at
the narrower end of barrel. There is no termin...
... measured by a TLC assay. Table 1 shows
that 5-halogenated analogues are good substrates and
have the highest activities. The iodo atom has the same
van der Waals radius as the methyl group of ... molecule.
Substrate binding
The substrate dT binds to the enzyme in a similar way
to the dT moiety of the feedback inhibitor dTTP
(Figs 1B and 2) and has the same interact...
... PA-oligosaccharides,
GalNAca1-3(Fuca1-2)Galb1-3(Fuca1-4)GlcNAcb1-3Galb1-
4Glc-PA and Neu5Aca2-6Galb1-4GlcNAcb1-2Mana1-
6(Neu5Aca2-3Galb1-3(Neu5Aca2-6)GlcNAcb1-4(Neu5Aca2-
6Galb1-4GlcNAcb1-2)Mana1-3)Manb1-4GlcNAcb1-4Glc-
NAc-PA ... (C)
Aromatic side chains and one backbone trace of the NMR struc-
tures for the N-terminal domain. (D) Aromatic side chains and one
backbone trace of the...
... Ding-Kwo Chang
Institute of Chemistry, Academia Sinica, Taipei, Taiwan, Republic of China
The structure and membrane interaction of the internal
fusion peptide (IFP) fragment of the avian sarcoma and
leucosis ... Æmin
)1
with a time constant of 2 s, step resolution
of 0.1 nm, and bandwidth of 1 nm. The final spectra were
taken from the average of five s cans. Th...
... functioning as nitroreductase and ferric
reductase is capable of catalyzing the Fenton reaction.
FEBS J 274, 1318–1327.
14 Kitamura M, Kojima S, Ogasawara K, Nakaya T,
Sagara T, Niki K, Miura K, Akutsu ... argon, and
NADH or NADPH was used as the reductant. The
observed increase in absorption around 340 nm is
caused by the addition of NADH or NADPH. Based
on the amino acid com...
... transmembrane domain, and
an intracellular domain consisting of an ATP -binding
regulatory domain and a GCase catalytic domain [8].
ANP binding to the ECD stimulates the intracellular
GCase domain, thereby ... quaternary
arrangement of the ECD dimer without significant
intramolecular structure change. This change in the
quaternary structure causes an alteration i...
... PP2C
enzymes may also have additional domains, for exam-
ple, Arabidopsis thaliana ABI1 in which the catalytic
domain is fused to an EF-hand motif, and human STP
(HsSTP), which has an additional 8 kDa a- helical
domain ... histidine, aspar-
tate and asparagine side chains bind the metal ions,
whereas in PPM enzymes, aspartates and a glycine
backbone carbonyl coordinate the meta...
... and a C-terminal flavodoxin-like
domain (residues 253–404) containing FMN. Two
monomers assemble via a head-to-tail arrangement,
such that the b-lactamase and the flavodoxin domains
face each other, ... to as proximal iron,
and the other as distal iron. The distance between N5
of FMN and the proximal Fe of about 9 A
˚
is within a
suitable range to allow rapid electron tr...
... and
catalyse a broad range of reactions mainly involving
the cleavage and the formation of C–C-bonds. For
instance, they catalyse nonoxidative and oxidative de-
carboxylations of 2-ketoacids, produce ... Benzaldehyde lyase (BAL) catalyzes cleavage and formation
of R-benzoin. BAL is known to accept several other substituted aro-
matic or aliphatic acyl-acceptors as substrates for...
... Rha
II
isthesiteofattachmentofthe
GlcNAc side chain. The NOESY data were in agreement
with the methylation analysis data, and the glycosylation
pattern was further confirmed by the
13
CNMRchemical
shift ... composition and similar structures differing from
each other in the position of substitution of one of the
rhamnose residues (Rha
IV
)inthemainchainandthesite
of attac...