Báo cáo khoa học: Acidic extracellular pH increases calcium influx-triggered phospholipase D activity along with acidic sphingomyelinase activation to induce matrix metalloproteinase-9 expression in mouse metastatic melanoma pot
... 3183
Acidic extracellular pH increases calcium in ux-triggered
phospholipase D activity along with acidic
sphingomyelinase activation to induce matrix
metalloproteinase-9 expression in mouse metastatic
melanoma
Yasumasa ... attenu-
ated matrix metalloproteinase-9 induction but did not affect calcium in ux.
We found that acidic sphingomy...
... resolved. To ensure that the flotation was due to binding to light vesicles, 1% Triton X-100 and 1% SDS were added to dissolve the
membranes, as demonstrated for BIP, synaptophysin and biotinylated ... p25a p25aDN, p25aDC and the p25a core, corre-
sponding to residues 44–156, were cloned into pET1 1d vector, expressed in E. coli and purified. The purified proteins were subjected to...
... aB-crystallin.
Reduction-induced aggregation of the insulin B-chain
Light scattering due to DTT-induced amorphous
aggregation and precipitation of the insulin B-chain
commenced after 10 min and reached ... aB-crystallin protects against the
DTT-induced aggregation of insulin, and this
activity is enhanced by Arg-HCl. Insulin
(
, 0.25 mgÆmL
)1
) was incubated at 37 °C
in 50 m
M phosphate...
... Preincuba-
tion of CatE with the antibody showed a dose-depend-
ent inhibition of antibody binding (IC
50
¼ 48.6 ng;
Fig. 2C). Increasing concentrations of CatD did not
affect antibody binding. This experiment ... CatD is reported to be
involved in processing MHC II-associated invariant
chain [24] in antigen processing and presentation
[25,26]. CatE is also reported to be involved...
... this involves storing energy as fat during
periods of an abundant food supply to hedge against
periods of food shortage. Today, humans have pushed
storage too far, to the point of widespread obesity.
Although ... preferable to starvation, this state
frequently leads directly or indirectly to serious pathol-
ogies including diabetes and heart disease. Interven-
tions to diminish adip...
... stud-
ies, the human control and DRPLA brain tissues were fixed
in 10% formalin and embedded in paraffin. The sections
from the brain tissues were immunostained with ATN1
antibodies as described ... and Gerontology, Aichi,
Japan
Introduction
The polyglutamine (polyQ) diseases are a group of
hereditary neurodegenerative disorders that include
Huntington’s disease (HD), dentatorubral-pallid...
... locali-
zation within the developing neural tube [3,62,121].
In addition, mHSF2 is associated with DNA binding
in the developing brain, including cortex, striatum,
olfactory bulbs and mesencephalon, ... before and after birth in mouse brains
[122,124]. No HSF4 DNA-binding activity was
reported in the developing mouse brain so far. How-
ever, Hsf4b is expressed in the adult...
... its inhibitory
effects at or before the adipogenic commitment stage
and that the IDM-induced genes appear to overcome
the inhibitory effects of miR-27.
In order to determine whether miR-27
inhibits ...
+IDM
+IDM
A
B
C
D
Fig. 4. Inhibition of expression of PPARc and C ⁄ EBPa in preadipocytes by miR-27 . (A) 3T3-L1 preadipocytes were transfected with miRNAs,
and then induced...
... replaced by Cys in
order to introduce a symmetry-related disulfide bond
with the Cys residue at position 13 of the other sub-
unit [25,26], yielding a covalently bridged dimer. The
oxidized and reduced ... contained 100 mm triethanol-
amine, 5 mm EDTA, 0 .5 mm NADH and a-glycerolphosphate
dehydrogenase (20 lgÆmL
)1
) and 0.10–3.0 mm glyceralde-
hyde 3-phosphate. Enzyme activity was det...
... stressed the importance of
light-induced protein degradation in phytochrome-con-
trolled signalling and showed data on mutants, isolated
in a custom-designed genetic screen, that are impaired
in ... showed that the CUL4–DDB1–
DDB2 complex plays a role in nucleotide excision repair
of UV-C-induced DNA damage and that this activity is
controlled by the ATR kinase [68]. In addition,...