Báo cáo khoa học: Coordination of three and four Cu(I) to the a- and b-domain of vertebrate Zn-metallothionein-1, respectively, induces significant structural changes doc
... arsenic atoms in FlAsH
enables it to bind with high affinity and specificity to the
TC motif introduced into a variety of different proteins
[15]. The binding of FlAsH reagent and also the quan-
tum ... ¼ðPrP
tot
À PrP
nat
Þ=PrP
tot
where PrP
nat
represents the nonconverted amount and
PrP
tot
the total amount (converted plus nonconverted) of
PrP.
Construction of retr...
... subset of
human lung cancers
To gain further insights into the role of miR-12 5a-5 p
in lung carcinogenesis and to examine the clinical rele-
vance of our findings, we investigated the expression
of ... added to the insert. Forty-eight hours after
seeding, the cells on the upper surface of the membrane
were removed using cotton buds. Cell monolayers on the
low...
... common type of entailment
rules, in which the left-hand-side of the rule (LHS)
and the right-hand-side of the rule (RHS) are propo-
sitional templates - a proposition, where one or both
of the arguments ... Fragment of the entailment graph for ‘asthma’
(top), and its conversion to a DAG (bottom).
3.2 Exploration Scheme
The objective of the exploration scheme...
... stoichiometries
of three and four for the a- and b-domain, respectively.
The presence of Cu(I) led to dramatic conformational
changes of both polypeptide folds. Cu(I) stoichio-
metries of up to six Cu(I) ... mm. Coordination of Cu(I) to
acetonitrile prevented undesired Cu(I) disproportionation
into Cu(II) and elemental copper. Four and three...
... were
shown to be A-type reductases catalysing the transfer of deuterium from
the 4(R) position of NADPH to the 1¢ (S) position of the substrate. These
results are in agreement with the known three- dimensional ... temperature, and a dual
set of
13
C-NMR signals is therefore observed for
each of the
13
C-labelled carbon atoms of the ribosyl
side chain [12]. Mor...
... in
the genome of living cells is naturally stochastic on
the one hand, and influenced by the actual structural
and functional state of the given chromatin domain
on the other. Moreover, some of the ... Hence, the response of a cancer cell to cisp-
latin seems to be rather complex, and its relationship
to the status of the p53 family proteins does not
a...
... Addition of the fragment S20 cp led to the for-
mation of new products detected as three additional
bands (lanes 3 and 4). These signals correspond to the
29- and 35-mer resulting from ligation of the ... the oxygen atom of the free 5¢-OH
group of one RNA fragment attacks the phosphorous
of the cyclic 2¢,3¢-phosphate group of another, result-
ing in lig...
... about the
topology of the binding site of the antibody and particularly
to appreciate the size of the cavity left around the iron atom
and which kind of ligands it can accommodate; second, to
measure ... in the presence of increasing concentra-
tions of imidazole and compared to that of the a,a -and
a,b-1,2-Fe(DoCPP ))13G10 complexes. With both a,b -...
... between the C tails of
b and c and the ubiquitin ligase Nedd4. The WW domains
of Nedd4 bind to the proline-rich PY motifs of b and
cENaC, leading to channel ubiquitination, internalization
and degradation ... quantified by
phosphorimaging of the band corresponding to the full-
length protein, and normalizing to the Coomassie blue
staining of the same...
... level
to be of the highest interest, in order to shed light on
the possible physiological functions of MTs in this
organism and to further the understanding of the
forces driving MT isoform differentiation, ... for the native and recombi-
nant Cd(II)–CeMT2 isoform [16,20]. The slight blue-
shift of the spectrum of Cd
6
Zn
1
–CeMT1 in relation to
that of C...