Báo cáo khoa học: Coordination of three and four Cu(I) to the a- and b-domain of vertebrate Zn-metallothionein-1, respectively, induces significant structural changes doc

Báo cáo khoa học: Tetracysteine-tagged prion protein allows discrimination between the native and converted forms pptx

Báo cáo khoa học: Tetracysteine-tagged prion protein allows discrimination between the native and converted forms pptx

... arsenic atoms in FlAsH enables it to bind with high affinity and specificity to the TC motif introduced into a variety of different proteins [15]. The binding of FlAsH reagent and also the quan- tum ... ¼ðPrP tot À PrP nat Þ=PrP tot where PrP nat represents the nonconverted amount and PrP tot the total amount (converted plus nonconverted) of PrP. Construction of retr...
Ngày tải lên : 06/03/2014, 11:20
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Báo cáo khoa học: Epidermal growth factor receptor-regulated miR-125a-5p – a metastatic inhibitor of lung cancer potx

Báo cáo khoa học: Epidermal growth factor receptor-regulated miR-125a-5p – a metastatic inhibitor of lung cancer potx

... subset of human lung cancers To gain further insights into the role of miR-12 5a-5 p in lung carcinogenesis and to examine the clinical rele- vance of our findings, we investigated the expression of ... added to the insert. Forty-eight hours after seeding, the cells on the upper surface of the membrane were removed using cotton buds. Cell monolayers on the low...
Ngày tải lên : 07/03/2014, 00:20
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Báo cáo khoa học: "Entailment-based Text Exploration with Application to the Health-care Domain" pot

Báo cáo khoa học: "Entailment-based Text Exploration with Application to the Health-care Domain" pot

... common type of entailment rules, in which the left-hand-side of the rule (LHS) and the right-hand-side of the rule (RHS) are propo- sitional templates - a proposition, where one or both of the arguments ... Fragment of the entailment graph for ‘asthma’ (top), and its conversion to a DAG (bottom). 3.2 Exploration Scheme The objective of the exploration scheme...
Ngày tải lên : 07/03/2014, 18:20
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Báo cáo khoa học: Coordination of three and four Cu(I) to the a- and b-domain of vertebrate Zn-metallothionein-1, respectively, induces significant structural changes doc

Báo cáo khoa học: Coordination of three and four Cu(I) to the a- and b-domain of vertebrate Zn-metallothionein-1, respectively, induces significant structural changes doc

... stoichiometries of three and four for the a- and b-domain, respectively. The presence of Cu(I) led to dramatic conformational changes of both polypeptide folds. Cu(I) stoichio- metries of up to six Cu(I) ... mm. Coordination of Cu(I) to acetonitrile prevented undesired Cu(I) disproportionation into Cu(II) and elemental copper. Four and three...
Ngày tải lên : 07/03/2014, 09:20
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Tài liệu Báo cáo khoa học: 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5¢-phosphate synthases of fungi and archaea docx

Tài liệu Báo cáo khoa học: 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5¢-phosphate synthases of fungi and archaea docx

... were shown to be A-type reductases catalysing the transfer of deuterium from the 4(R) position of NADPH to the 1¢ (S) position of the substrate. These results are in agreement with the known three- dimensional ... temperature, and a dual set of 13 C-NMR signals is therefore observed for each of the 13 C-labelled carbon atoms of the ribosyl side chain [12]. Mor...
Ngày tải lên : 18/02/2014, 18:20
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Tài liệu Báo cáo khoa học: DNA modification with cisplatin affects sequence-specific DNA binding of p53 and p73 proteins in a target site-dependent manner pptx

Tài liệu Báo cáo khoa học: DNA modification with cisplatin affects sequence-specific DNA binding of p53 and p73 proteins in a target site-dependent manner pptx

... in the genome of living cells is naturally stochastic on the one hand, and influenced by the actual structural and functional state of the given chromatin domain on the other. Moreover, some of the ... Hence, the response of a cancer cell to cisp- latin seems to be rather complex, and its relationship to the status of the p53 family proteins does not a...
Ngày tải lên : 19/02/2014, 05:20
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Tài liệu Báo cáo khoa học: Efficient RNA ligation by reverse-joined hairpin ribozymes and engineering of twin ribozymes consisting of conventional and reverse-joined hairpin ribozyme units ppt

Tài liệu Báo cáo khoa học: Efficient RNA ligation by reverse-joined hairpin ribozymes and engineering of twin ribozymes consisting of conventional and reverse-joined hairpin ribozyme units ppt

... Addition of the fragment S20 cp led to the for- mation of new products detected as three additional bands (lanes 3 and 4). These signals correspond to the 29- and 35-mer resulting from ligation of the ... the oxygen atom of the free 5¢-OH group of one RNA fragment attacks the phosphorous of the cyclic 2¢,3¢-phosphate group of another, result- ing in lig...
Ngày tải lên : 20/02/2014, 01:20
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Tài liệu Báo cáo khoa học: Coordination chemistry of iron(III)±porphyrin±antibody complexes In¯uence on the peroxidase activity of the axial coordination of an imidazole on the iron atom ppt

Tài liệu Báo cáo khoa học: Coordination chemistry of iron(III)±porphyrin±antibody complexes In¯uence on the peroxidase activity of the axial coordination of an imidazole on the iron atom ppt

... about the topology of the binding site of the antibody and particularly to appreciate the size of the cavity left around the iron atom and which kind of ligands it can accommodate; second, to measure ... in the presence of increasing concentra- tions of imidazole and compared to that of the a,a -and a,b-1,2-Fe(DoCPP ))13G10 complexes. With both a,b -...
Ngày tải lên : 21/02/2014, 03:20
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Tài liệu Báo cáo khoa học: Casein kinase 2 specifically binds to and phosphorylates the carboxy termini of ENaC subunits ppt

Tài liệu Báo cáo khoa học: Casein kinase 2 specifically binds to and phosphorylates the carboxy termini of ENaC subunits ppt

... between the C tails of b and c and the ubiquitin ligase Nedd4. The WW domains of Nedd4 bind to the proline-rich PY motifs of b and cENaC, leading to channel ubiquitination, internalization and degradation ... quantified by phosphorimaging of the band corresponding to the full- length protein, and normalizing to the Coomassie blue staining of the same...
Ngày tải lên : 21/02/2014, 03:20
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Báo cáo khoa học: Caenorhabditis elegans metallothionein isoform specificity – metal binding abilities and the role of histidine in CeMT1 and CeMT2 potx

Báo cáo khoa học: Caenorhabditis elegans metallothionein isoform specificity – metal binding abilities and the role of histidine in CeMT1 and CeMT2 potx

... level to be of the highest interest, in order to shed light on the possible physiological functions of MTs in this organism and to further the understanding of the forces driving MT isoform differentiation, ... for the native and recombi- nant Cd(II)–CeMT2 isoform [16,20]. The slight blue- shift of the spectrum of Cd 6 Zn 1 –CeMT1 in relation to that of C...
Ngày tải lên : 07/03/2014, 02:20
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