Báo cáo khoa học: C fi G base mutations in the CArG box of c-fos serum response element alter its bending flexibility Consequences for core-SRF recognition potx
... SRE
Gfos
5¢-d(GGATGTgCATATTAGGACAT)-3¢ and
SRE
GGfos
5¢-d(GGATGTggATATTAGGACAT)-3¢ have
one (C fi G) and two (CC fi GG) mutations, respectively,
at the end of the CArG consensus sequence underlined
above (mutations ... restricted range
of conformational space. Thus, for the CArG box
of SRE
GGfos
, the rigid G
)4
A
)3
step inhibits (by
mechanical locking) the...
... triggered by CdTe QDs in glial cells
depends on the de novo synthesis of lipids and
phosphoinositide 3-kinase (PI3K) signaling
Cytoplasmic LDs contain neutral lipids, including
triacylglycerols, ... than the control (Fig. 4I versus Fig. 4C, K). Cells
treated with CoCl
2
in the absence of serum did not
contain LDs, suggesting that the activation of
PI3K ⁄ Akt by trophic fac...
... 19N
[17]. The linker-encoding region of that plasmid was
replaced with DNA encoding GEDVVCCSMSYGAG (to
yield the ‘ 2C zymogen) or GEDVVACSMSYGAG (to
yield the ‘ 1C zymogen) by using the QuikChange muta-
genesis ... that of the unactivated 2C zymogen,
and the difference is again the result of both a decrease
in k
cat
and an increase in K
m
. The increase in k
cat
o...
... in Fig. 2. In the absence of MgCl
2
, the
affinity of Fpg for the THF :C ligand was 1.6-fold higher
than for the THF:A ligand. The presence of Mg
2+
had
little effect on the binding of Fpg to the ... no Mg
2+
(C ⁄ A specificity of
1.5 for DNA glycosylase reaction and 0.78 for AP
lyase reaction) and the increase in the preference for
8-oxoG :C with...
... length of the
N-terminal fragment was definned as the length of the
amino acid sequence preceding the next domain in
the arrangements, expressed as the percentage of the calcu-
lated average length ... C terminus
Length of the N terminus in % of the deleted domain
Length of the C terminus in % of the deleted domain
Pfam, N−terminus Pfam, C −termin...
... FEBS
genomic DNA using forward (5¢-GGAGGGATCCCATATG
AAGTCTAAATTTAAATTAACG-3¢) and reverse (5¢-GC
GTG
CTCGAGCGATCCATGGAGCCGTA-3¢) primers
that incorporated BamHI and NdeI restriction sites in the
5¢-end ... activator
of the mauF promoter were amplified from genomic DNA
using forward (5¢ -TTGGT
AAGCTTGGGCATTTCTGAT
CGGGTCGC-3¢) and reverse (5¢-AAAC
CATATGACGCC
TCCTCTCGCT-3¢) primers that...
... ap-
proaches is based on the learning aspect during
the construction of the morphological model. If
the data for training the model has the same struc-
ture as the desired output of the morphological
analysis, ... described below, we chose the South
African language Zulu since our research work
mainly aims at creating morphological resources
for under-resourced indig...
... before the actual agree-
ment and disagreement classification. Since tag se-
quences are known during training, the inference of
a model for sequence labels is no more difficult than
inferring a model ... retaining the hand-labeled data of
a meeting for testing and the rest of the data for
training. Table 7 summarizes the performance of
our classifier with the differe...
... cloning of the chimeric cDNA into
the plant transformation vector CRC-179. CRC-179 was
derived from the lbc3-GUS vector [19] by removal of a
DNA fragment containing the Gmlbc3 promoter, the
gusA ... IF.
Binding of IF to cubilin
Speci c binding of IF–Cbl complex to the immobilized
receptor cubilin was conducted on a BIAcore 2000
equipment as described earlier [24]. In sh...
... language interfaces to databasesl"
has resulted in this panel, which is supposed to
debate the necessity of continuing work in this
area rather than to debate technical issues in the ... language interfaces
(limiting that phrase, for the moment, to mean
typed English sentences) to various kinds of
information systems. At one end of this spectrum
is simple, sing...