Báo cáo khoa học: C fi G base mutations in the CArG box of c-fos serum response element alter its bending flexibility Consequences for core-SRF recognition potx

Báo cáo khoa học: C fi G base mutations in the CArG box of c-fos serum response element alter its bending flexibility Consequences for core-SRF recognition potx

Báo cáo khoa học: C fi G base mutations in the CArG box of c-fos serum response element alter its bending flexibility Consequences for core-SRF recognition potx

... SRE Gfos 5¢-d(GGATGTgCATATTAGGACAT)-3¢ and SRE GGfos 5¢-d(GGATGTggATATTAGGACAT)-3¢ have one (C fi G) and two (CC fi GG) mutations, respectively, at the end of the CArG consensus sequence underlined above (mutations ... restricted range of conformational space. Thus, for the CArG box of SRE GGfos , the rigid G )4 A )3 step inhibits (by mechanical locking) the...
Ngày tải lên : 07/03/2014, 09:20
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Báo cáo khoa học: Nanoparticles can induce changes in the intracellular metabolism of lipids without compromising cellular viability docx

Báo cáo khoa học: Nanoparticles can induce changes in the intracellular metabolism of lipids without compromising cellular viability docx

... triggered by CdTe QDs in glial cells depends on the de novo synthesis of lipids and phosphoinositide 3-kinase (PI3K) signaling Cytoplasmic LDs contain neutral lipids, including triacylglycerols, ... than the control (Fig. 4I versus Fig. 4C, K). Cells treated with CoCl 2 in the absence of serum did not contain LDs, suggesting that the activation of PI3K ⁄ Akt by trophic fac...
Ngày tải lên : 07/03/2014, 00:20
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Báo cáo khoa học: A ribonuclease zymogen activated by the NS3 protease of the hepatitis C virus potx

Báo cáo khoa học: A ribonuclease zymogen activated by the NS3 protease of the hepatitis C virus potx

... 19N [17]. The linker-encoding region of that plasmid was replaced with DNA encoding GEDVVCCSMSYGAG (to yield the ‘ 2C zymogen) or GEDVVACSMSYGAG (to yield the ‘ 1C zymogen) by using the QuikChange muta- genesis ... that of the unactivated 2C zymogen, and the difference is again the result of both a decrease in k cat and an increase in K m . The increase in k cat o...
Ngày tải lên : 07/03/2014, 11:20
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Tài liệu Báo cáo khoa học: Ionic strength and magnesium affect the specificity of Escherichia coli and human 8-oxoguanine-DNA glycosylases pdf

Tài liệu Báo cáo khoa học: Ionic strength and magnesium affect the specificity of Escherichia coli and human 8-oxoguanine-DNA glycosylases pdf

... in Fig. 2. In the absence of MgCl 2 , the affinity of Fpg for the THF :C ligand was 1.6-fold higher than for the THF:A ligand. The presence of Mg 2+ had little effect on the binding of Fpg to the ... no Mg 2+ (C ⁄ A specificity of 1.5 for DNA glycosylase reaction and 0.78 for AP lyase reaction) and the increase in the preference for 8-oxoG :C with...
Ngày tải lên : 18/02/2014, 18:20
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Tài liệu Báo cáo khoa học: Domain deletions and substitutions in the modular protein evolution doc

Tài liệu Báo cáo khoa học: Domain deletions and substitutions in the modular protein evolution doc

... length of the N-terminal fragment was definned as the length of the amino acid sequence preceding the next domain in the arrangements, expressed as the percentage of the calcu- lated average length ... C terminus Length of the N terminus in % of the deleted domain Length of the C terminus in % of the deleted domain Pfam, N−terminus Pfam, C −termin...
Ngày tải lên : 19/02/2014, 07:20
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Tài liệu Báo cáo khoa học: Tryptophan tryptophylquinone cofactor biogenesis in the aromatic amine dehydrogenase of Alcaligenes faecalis Cofactor assembly and catalytic properties of recombinant enzyme expressed in Paracoccus denitrificans pptx

Tài liệu Báo cáo khoa học: Tryptophan tryptophylquinone cofactor biogenesis in the aromatic amine dehydrogenase of Alcaligenes faecalis Cofactor assembly and catalytic properties of recombinant enzyme expressed in Paracoccus denitrificans pptx

... FEBS genomic DNA using forward (5¢-GGAGGGATCCCATATG AAGTCTAAATTTAAATTAACG-3¢) and reverse (5¢-GC GTG CTCGAGCGATCCATGGAGCCGTA-3¢) primers that incorporated BamHI and NdeI restriction sites in the 5¢-end ... activator of the mauF promoter were amplified from genomic DNA using forward (5¢ -TTGGT AAGCTTGGGCATTTCTGAT CGGGTCGC-3¢) and reverse (5¢-AAAC CATATGACGCC TCCTCTCGCT-3¢) primers that...
Ngày tải lên : 19/02/2014, 07:20
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Tài liệu Báo cáo khoa học: "Enhanced word decomposition by calibrating the decision threshold of probabilistic models and using a model ensemble" pdf

Tài liệu Báo cáo khoa học: "Enhanced word decomposition by calibrating the decision threshold of probabilistic models and using a model ensemble" pdf

... ap- proaches is based on the learning aspect during the construction of the morphological model. If the data for training the model has the same struc- ture as the desired output of the morphological analysis, ... described below, we chose the South African language Zulu since our research work mainly aims at creating morphological resources for under-resourced indig...
Ngày tải lên : 20/02/2014, 04:20
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Tài liệu Báo cáo khoa học: "Identifying Agreement and Disagreement in Conversational Speech: Use of Bayesian Networks to Model Pragmatic Dependencies" docx

Tài liệu Báo cáo khoa học: "Identifying Agreement and Disagreement in Conversational Speech: Use of Bayesian Networks to Model Pragmatic Dependencies" docx

... before the actual agree- ment and disagreement classification. Since tag se- quences are known during training, the inference of a model for sequence labels is no more difficult than inferring a model ... retaining the hand-labeled data of a meeting for testing and the rest of the data for training. Table 7 summarizes the performance of our classifier with the differe...
Ngày tải lên : 20/02/2014, 16:20
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Tài liệu Báo cáo khoa học: Human intrinsic factor expressed in the plant Arabidopsis thaliana doc

Tài liệu Báo cáo khoa học: Human intrinsic factor expressed in the plant Arabidopsis thaliana doc

... cloning of the chimeric cDNA into the plant transformation vector CRC-179. CRC-179 was derived from the lbc3-GUS vector [19] by removal of a DNA fragment containing the Gmlbc3 promoter, the gusA ... IF. Binding of IF to cubilin Speci c binding of IF–Cbl complex to the immobilized receptor cubilin was conducted on a BIAcore 2000 equipment as described earlier [24]. In sh...
Ngày tải lên : 21/02/2014, 00:20
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Tài liệu Báo cáo khoa học: "THERE STILL IS GOLD IN THE DATABASE MINE" potx

Tài liệu Báo cáo khoa học: "THERE STILL IS GOLD IN THE DATABASE MINE" potx

... language interfaces to databasesl" has resulted in this panel, which is supposed to debate the necessity of continuing work in this area rather than to debate technical issues in the ... language interfaces (limiting that phrase, for the moment, to mean typed English sentences) to various kinds of information systems. At one end of this spectrum is simple, sing...
Ngày tải lên : 21/02/2014, 20:20
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