... in the
product chain length determination mechanism of
type II GGPS. Moreover, this result suggests that the
other subunit of the homooligomeric enzyme is
involved in the product chain length determination
mechanism. ... information on the product determination mecha-
nism of type II GGPS. (A) The direction of the geranylgeranyl chain...
... similar
arrangement, and the side chain of Ser97 in VPRK is
too short. One side of the loop is involved in the bind-
ing of the P2–P4 residues of a substrate. The other side
of the loop is close to Arg94 ... considered to display differences in the fold of
the main chains. None of these includes any of the
binding sites, but some are located in the vicini...
... To
overcome the fact that the prosa z-score is dependant of the
length of the amino acid sequence, the z-score was
normalized using the natural logarithm of the sequence
length [39]. The resulting ... distribution of the
percentage best model, for each of the 672 best models, is presented in
the left panel. Of the six template structures used, 54% of...
... changed either in the presence or
absence of the metal cofactor. By contrast, the sub-
strate binding affinity of the RNase H domain proba-
bly increases in the presence of the metal cofactor
compared ... sequences of the oligonucleotides cleaved
by the enzyme are shown. The differences in the lengths of the arrows reflect relative cleavage intensities at the p...
... Mechanism of product
chain length determination and the role of a flexible loop
in Escherichia coli undecaprenyl-pyrophosphate synthase
catalysis. J Biol Chem 276, 47474–47482.
Product chain length of ... the
length of prenyl products, indicating a requirement for
insertion of specific amino acid residues for lengthen-
ing the ultimate product chain. Moreo...
... G
4
and the sidechain of Asn122. In the second model
(Fig. 1B) the structure of the hexanucelotide is, on the
whole, unchanged and the majority of the interactions that
were seen in the first ... attributed solely to the change in
charge and size of the sidechain of this single residue, the
crystal structure of RTA N122A was solved. The RMSD
from the C
a
a...
... of propionates of heme, was mutated to Tyr,
and the thermodynamic and kinetic properties of the mutant (F166Y) were
compared with those of the wild -type (WT) enzyme. The mid-point
potential of ... because of the weaker domain interaction of
this mutant enzyme. The F166Y mutant is, however,
useful for monitoring the electron transfer step of
CDH, because the...
... loss
of these interactions even though the amounts of cellular
crBAP31 and c-actin did not apparently change (Fig. 5B).
These findings, coupled with the strong enrichment of the
c-isoform of actin ... have arisen at the
genetic level (by the presence of two or more allelic copies of
the gene of interest) rather than by the presence of low-level
amount of contami...
... During the monitoring period, details of
the total number of all prescribed drugs on each day were
recorded.
MEs were assessed by type and patient outcome. The type of
error was categorised by the ... (where the patient received the
drug). The intercepted errors were scored separately on the
basis of their possible impact on the patient, if the prescription
had...
... intravenous
lines). The observers were not involved in the daily care of the
patients, and ICU physicians were not aware of this part of the
observation. As a consequence, the clinical relevance of the
predefined ... for other infections, and the
absence of radiological signs of pulmonary congestion might
have resulted in another fluid therapy regimen.
We did not sco...