... at the
entrance of the cleft of EcoMBP, but the distance
between Od2 of Asp209 and Ng2 of Arg344 is only
10.2 A
˚
.InTliTMBP, the cleft is the smallest among
the three proteins (Fig. 7C). The ... half-buried, and the form of the cleft is markedly
different from those of TvuCMBP and EcoMBP.
These observations suggest that the structure of the
N -domain part...
... respectively, of that of HPRT. This low activity
could be explained by the fact that PRTFDC1 has a Gly in the position of
the proposed catalytic Asp of HPRT. In PRTFDC1, a water molecule at
the position of ... strength [20].
In the present study, we report the structural and
functional characterization of the human HPRT
homolog PRTFDC1. To shed light on the poten...
... hinge domains
The composition of the two hinges that connect the
FMN subdomain in NOS enzymes (H1 and H2 in
Fig. 5) defines the allowable movements of the FMN
subdomain and thus controls the FMN–NOSoxy ... in
NOS, the second electron is provided to the heme-
dioxy species by a bound H
4
B cofactor rather than by
the flavoprotein domain [16]. The H
4
B radical is then
r...
... nature. The growing body
of sequence and structural data on these well-studied
enzymes affords an opportunity to evaluate the conse-
quences of mutations. In the case of TIM, only nine of
the 220–250 ... method
for the quantitation of microgram quantities of protein
utilizing the principle of protein dye binding. Anal
Biochem 72, 248–254.
54 Oesper P & Meyer...
... evidence
supporting a structural interruption of the geometry
of the recognition helix for either Antp or the TTF-1
HD, the anomalous amide exchange pattern and the
NOE connectivity data of the C-terminal ... conflict with the
global interpretation of the data. With the exclusion of
the N-terminal octapeptidyl and C-terminal nonapept-
idyl fragments of Glu...
... proton from the catalytic Cys pres-
ent at the N-terminus of the helix to the His of the
dyad [19,50,51]. The first stage of catalysis is mediated
by the highly active thiolate ion of the Cys. Biochemi-
cal ... erva-
tamin-A, the P3 moiety of both the molecules of the
asymmetric unit runs along the extended backbone of
residues 65–64 and is partly exposed...
... urchin EGF-like protein and
bone morphogenic protein 1 [16]. These proteins are
often referred to as the prototype CUB domains. Like
the prototype CUB domains, the PDGF-C and -D
CUB domains span ... the
prototypic CUB domains, respectively (Fig. 2) [8]. The
CUB domains of PDGF-C and -D share 55%
sequence identity. CUB domains are assembled as a
Fig. 2. The CUB domain. Th...
... motif’, a structurally uncharacterized motif.
Fig. 2. An overview of the PhKc domain structure. The first 296
residues of the subunit encode a protein kinase domain. The struc-
ture of the constitutively ... these structures a
C-terminal extension to the protein kinase folds back
on the kinase domain and interferes with the substrate
binding sites. In the cas...
... about the function of the protein. The
Fig. 1. (A) Cartoon representation of CagZ
protein fold. The L-shape is clearly visible.
(B) Two views of the electrostatic potential
surface of CagZ. In the ... side of the peanut. The results of the crystallo-
graphic model of CagS do not show any clear evidence
of architectural similarity to other known structures,...
... C-lobe
domain containing 1) [9]. The KIND domain in these
proteins is localized to the N-terminal region, and their
specific functional domains are located in the C-termi-
nal region. The C-lobe of protein ... central domain of MAP2
contain the v-KIND binding core module
MAP2 consists of three main structural domains: the
cAMP-dependent protein kinase regulatory s...