Báo cáo khoa học: Molecular cloning and characterization of

Báo cáo khoa học: Molecular cloning and characterization of two soybean protein disulﬁde isomerases as molecular chaperones for seed storage proteins doc

... (2008) A novel plant protein disulﬁde isomerase family homologous to animal P5: molecular cloning and characterization as a functional protein for folding of soybean seed storage proteins. FEBS J ... substrate proteins. Mapping of associa- tion sites of chaperones and PDI family proteins along individual substrate polypeptides will be necessary for clar...

Ngày tải lên : 07/03/2014, 05:20

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Báo cáo khoa học: cDNA cloning and characterization of a novel calmodulinlike protein from pearl oyster Pinctada fucata potx

... and CaM, it is possible that these proteins share potential binding sites or target proteins. Potential CaLP binding and CaM binding proteins in the extracts of the mantle and gill tissues, two ... as a single band with an apparent molecular weight of approximately 18 kDa and 14 kDa, respectively, whereas in the absence of cal- cium, the apparent molecular mass was...

Ngày tải lên : 16/03/2014, 23:20

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Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebraﬁsh, Danio rerio docx

... used as an internal control for RT-PCR. PCR condi- tions were as follows: one cycle of 94 °C for 3 min, 32 cycles of 94 °C for 30 s, 54 °C for 30 s and 72 °C for 30 s, followed by one cycle of ... analysed for similarity with other known sequences using the fasta [25] and blast [26] suite of pro- grams. Homology analysis of the amino acid sequences was performed...

Ngày tải lên : 16/02/2014, 09:20

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Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf

... properties of SmSmad1 and SmSmad1B as described above. However, in the presence of SmSmad4 and the active mutant form of TbRI, both SmSmad2 and SmSmad4 interacted with GCN5, indicating the formation of ... yeast strain Y190, a HIS3 ⁄ LacZ reporter strain, was utilized in the yeast two- hybrid assays. Preparation of yeast competent cells and transformations were achieved u...

Ngày tải lên : 18/02/2014, 16:20

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Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon doc

... from leaves was composed of two subunits with different N-terminal amino acid sequences. Analysis of the corresponding cDNA revealed that these two subunits have molecular masses of 21.3 and 23.5 kDa and ... 249–252. 35 Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein- dye bi...

Ngày tải lên : 20/02/2014, 03:20

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Tài liệu Báo cáo khoa học: The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox properties ppt

... visible, as well as a faint band at 37 kDa, suggesting the presence of dimers. All of the bands stained positively for heme c (Fig. 1B) but only the high molecularmassbands(‡ 55 kDa) stained for nitrite ... combination of the two subunits that match the smallest molecular mass is a 2 b 2 . Overnight incubation of the protein in this detergent led to a decrease of the a...

Ngày tải lên : 21/02/2014, 00:20

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Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a sialic acid speciﬁc lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf

... preparation of glycoproteins. De-O- acetylation of glycoproteins was performed following the procedures of Sarris and Palade [30] and Schauer [6]. A solution of 750 lL of glycoprotein (5 mgÆmL )1 ) was ... for 3 h at 37 °C. As a control, glycoproteins were treated similarly without sialidase. The HAI assay was performed with puriﬁed lectins for treated and untreated glyc...

Ngày tải lên : 21/02/2014, 00:20

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Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulﬁde reductase from methanogenic archaea pdf

... does an aspartate residue replace one of the ﬁve cysteines present in this motif [25]. The AF501 protein has a calculated molecular mass of 38 k Da. The molecular mass of this protein was estimated by ... 1). The AF503 protein has a calculated molecular mass of 16.7 kDa. The protein contains three CxxCH sequence motifs characteristic of proteins th at co-ordinate hem...

Ngày tải lên : 21/02/2014, 03:20

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Báo cáo khoa học: Puriﬁcation and characterization of zebraﬁsh hatching enzyme – an evolutionary aspect of the mechanism of egg envelope digestion pot

... ZHE1 and analyzed by SDS ⁄ PAGE. After incubation for 2 min, bands with molecular masses of 43 and 39 kDa were observed in addition to undigested bands of ZP2 and ZP3. After incubation for 10 ... 281–289. 8 Yasumasu S, Iuchi I & Yamagami K (1994) cDNAs and the genes of HCE and LCE, two constituents of the medaka hatching enzyme. Dev Growth Differ 36, 241–250. 9 K...

Ngày tải lên : 07/03/2014, 04:20

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Báo cáo khoa học: Identiﬁcation and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot

... domain as demonstrated in a yeast one-hybrid assay; it interacts with SmRXR1 in a yeast two- hybrid assay and in a glutathione S-transferase pull-down assay. Electrophoretic mobil- ity shift assay ... (GST pull-down assays) and in vivo (yeast two- hybrid and mammal cell assays) results. Likewise, the ability of the heterodimer to bind DNA is shown by in vitro (electrophoretic mobi...

Ngày tải lên : 07/03/2014, 11:20

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