... the substrate via two
elements in ClpA, one in the N-domain and the other
in the pore of the ClpA hexamer. In the case of short
unstructured peptides or unfolded proteins such as
casein, binding ... have analysed the role of
five highly conserved basic residues in the N-domain of ClpA by monitor-
ing the binding, unfolding and degradatio...
... atalytic
function of the C-terminal domain is not apparent [12], and
thus the role of the corresponding part of the gene is also
unknown. These g ene f usions togeth er with single-copy
genes of APXs and CCPs ... conserved arginine (Arg622 in
HalomarCPc) in t he corresponding position, indicating that
these domains lost their ability to co-ordinate the heme.
Furthe...
... amino acid biosynthesis. Much of
the generation of NADH during amino acid biosyn-
thesis takes place in the mitochondria. Because of the
block in the respiratory chain caused by the addition
of ... N
2
(containing less than 3 p.p.m. O
2
) at a flow
rate of 0.1 LÆmin
)1
per liter of medium, and a stir rate of
500 r.p.m. was maintained. In order to minimize the...
... using the main chain of
residues Ser12 fiThr70 in t he structure a lignment. (B) O verlap of the
X-ray structure with the 30 °C NMR structure using the main chain of
residues Ser12fiThr70 in the ... considerable number of questions remain
before we can solve the interaction at the atomic level and
understand t he role of the threonine side chains in ice
bin...
... we then analyzed the
genomic organization of the murine o-macs gene. The
mouse o-macs is located in the 7F1 region of chromosome 7
and is composed of 14 exons including the 5¢ -and3 ¢-non
coding ... guanine nucleotide releasing protein ND – Rat IGF binding protein 5 ND –
Rat FGF receptor activating protein 1 ND –
Rat PKC delta-binding protein ND –
Rat cellular retinoyl-bin...
... cells.
Referring to Smad4, there are significant levels of protein
in the nucleus of both cell lines already in the absence of
ligand. Cycloheximide treatment for 1 h prior to the
addition of NGF indicated ... confocal
microscopy. The projection of multiple sections is seen on the left for each panel to visualize the morphology of the cells. The middle row shows...
...
modifiers in the domain of cooking tasks. Much of
this is pertinent to an examination of extent, in par-
tieular the relation of different modifiers to the as-
peetual category of an event (according ... frequency in time units, as
in every 5 minutes.
Duration of an action - delimiting the
interval
Here, Karlin enumerates the following kinds of
modifier...
... except in the
characters' dialogs.
Behind the superficial dl~erence in the use
of tenses stands the more important one in the
basic meaning of the grammatical category of
tense. The standard ...
Hartley and Phoebe had been sent by
their mother to fix the tail v-~hve of the windmilL
b. In the great expanse of the prairie where
they lived,...
... was
imported and processed even in the absence of exoge-
nous ATP. The yield of pTic22 import in the absence
of ATP varied between 20–50% of that in the presence
of ATP. The import of pMGD1 was ... [
35
S]pTic22, [
35
S]pMGD1 and [
35
S]pSSU were added to the column in binding buffer and incubated for 45 min. The flow
through after incubation (Ft), the...
... proteins of the thylakoid lumen. In addition,
there are certain proteins that possess a thylakoid
membrane anchor but expose the bulk of the poly-
peptide chain and the prosthetic group involved in
electron ... absence of specific
translocase inhibitors (Fig. 5). A striking feature in the
targeting process of Rieske protein is the remarkably
slow sorting of the...