Báo cáo khoa học: Xenopus Rbm9 is a novel interactor of XGld2 in the cytoplasmic polyadenylation complex pptx
Báo cáo khoa học: Xenopus Rbm9 is a novel interactor of XGld2 in the cytoplasmic polyadenylation complex pptx
... However, the RRM-containing centraldomain of hRbm9 (amino acids 48–269) and aminoacids 269–350 of hRbm9 are not able to mediate the binding. These data suggest that a domain surroundingamino acid ... 2008 The Authors Journal compilation ª 2008 FEBS 503 Xenopus Rbm9 is a novel interactor of XGld2 in the cytoplasmic polyadenylation complex Catherine Papin*, Christel Rouget* and Elisabeth MandartCentre ... frame with the Gal4activation domain (Gal4AD) into the EcoRI site of the pADGal4 vector. pAD XRbm9DN: the XRbm9DN frag-ment was inserted in frame with Gal4AD into the EcoRIsite of the pADGal4...
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Tài liệu Báo cáo khoa học: KIPase activity is a novel caspase-like activity associated with cell proliferation doc
... standarddeviation. (B) The ability of the broad specificity caspase inhibitor, z-VAD-fmk, to inhibit KIPase activity in the indicated cell lines was assessedusing the same assay. The data are ... Ac-YVAD-AMC is a substrate for caspase 1;Ac-IETD-AMC is a substrate for caspase 8 and 10;Ac-LEHD-AMC is a substrate for caspases 2, 4, 5 and 9.Ac-DVPD-AMC, Ac-DPSD-AMC and Ac-ESQD-AMCare tetra peptide ... that KIPase is a novel enzyme distinct from any of the previously characterized human caspases. We showthat KIPase is active in a variety of cell lineages, its activity is associated with the...
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Báo cáo khoa học: Intermittent hypoxia is a key regulator of cancer cell and endothelial cell interplay in tumours pot
... 728–733.72 Hayakawa M, Miyashita H, Sakamoto I, Kitagawa M,Tanaka H, Yasuda H, Karin M & Kikugawa K (2003)Evidence that reactive oxygen species do not mediateNF-kappaB activation. EMBO ... each cycle of 30 min of reoxygenation,showing that HIF- 1a had not accumulated in the course of intermittent hypoxia cycles, and thereforethat it is its stabilization that is increased in theseconditions ... 3374–3378.101 Kanaan A, Farahani R, Douglas RM, Lamanna JC &Haddad GG (2006) Effect of chronic continuous orintermittent hypoxia and reoxygenation on cerebralcapillary density and myelination. Am J...
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Báo cáo khoa học: Structural evidence for a constant c11 ring stoichiometry in the sodium F-ATP synthase doc
... presence of SDS. The second type of aggregate appears as a ladder above the original c11band on SDS ⁄ PAGE andconsists of c11rings hosting varying amounts of the cmonomer. Aggregates are particularly ... significantly decreased the amount of the stable c11aggregates. A similar observation wasmade after incubating the sample for 5 min at 95 °CinSDS-containing loading buffer; this result confirms the extreme ... subunits. In combination with the asymmetric loca-tion of the central c subunit, and in accordance with the binding change mechanism [3], this arrangementsuggests a catalytic mechanism in which the...
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Báo cáo khoa học: "KNOWLEDGE ORGANIZATION AND APPLICATION: BRIEF COMIIENTS OF PAPERS IN THE SESSION " potx
... not clear how negation is handled. The main application is for the command and control of advanced graphics manioulators through natural language. Is there an implicit claim here that the KLONE ... representation. It is concerned with complete and partial matching procedures, especially for determining whether a particular instance satisfies the criteria for membership in a particular class. ... comments may turn out to be inappropriate or else they have already been taken care of in the full texts. In a couple of cases~ I had the benefit of reading some earlier longer related reports,...
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Tài liệu Báo cáo khoa học: Human Cdc45 is a proliferation-associated antigen pdf
... proliferation [55]. The assess-ment of cellular proliferation in histological material is a valuable component of conventional histopathologi-cal analysis and may be of major prognostic import-ance ... Westernblotting demonstrated that the precipitates containedapproximately equal amounts of the human Cdc45protein. Autoradiography of these samples showed a significant reduction in the radiolabelled ... Cdc45 is much less abundant than the minichromosome maintenance(Mcm) proteins in human cells, supporting the concept that origin binding of Cdc45 is rate limiting for replication initiation. We also...
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Tài liệu Báo cáo khoa học: Hu-K4 is a ubiquitously expressed type 2 transmembrane protein associated with the endoplasmic reticulum ppt
... membrane or with the membranes of intracellular organelles although theylack a transmembrane domain. They are attached to the cytoplasmic face of the membranes via palmitoylanchors [15] as is the ... between plasma membrane and intracellularorganelles [9] and is involved in the trafficking of integral membrane proteins from the Golgi apparatus.This function is inhibited by a phospholipase D inhib-itor ... the carboxy tail of Hu-K4. The apparent molecular mass of 65 kDa is higher than the calculated molecular mass of 55 kDa for Hu-K4 hinting at a post-translationalmodification, e.g. glycosylation, of...
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Báo cáo khoa học: Syndecan-4 is a signaling molecule for stromal cell-derived factor-1 (SDF-1)/ CXCL12 pptx
... SDF- 1a and phorbol 12-myristate-13-acetate (PMA) induced a rapid activation of p44 ⁄ 42MAPK and JNK ⁄ SAP kinase signaling in HeLa cellsby increasing phosphorylations of the respective pro-teins ... 0.05. The position of immunoglobulin chains is indicated by a star. Pro-tein bands with changes in tyrosine phosphorylation state are indicated by arrows.N. Charnaux et al. Syndecan-4 is an auxiliary ... (2002) Interaction of the CC-chemokine RANTES with glycosaminogly-cans activates a p44 ⁄ p42 mitogen-activated protein kin-ase-dependent signaling pathway and enhances humanSyndecan-4 is an auxiliary...
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Báo cáo khoa học: Cytochrome b6f is a dimeric protochlorophyll a binding complex in etioplasts doc
... study, the dataindicate that the phytyl chain in Chl is of centralimportance. Bleaching of the tetrayrrol moiety in Chlmaintained the Cyt b6f complex in a functional state[4]; however, a chlorophyll-less ... b6f complex of etioplasts exhibited an absorbance maxi-mum at 631 nm (Fig. 3). These findings argue for a replacement of Chl against Pchl in etioplasts. Replace-ment of a cofactor in the dimeric ... oper-ate in an alternative electron transfer chain. The NAD(P)H dehydrogenase complex, a peroxidase act-ing on reduced plastoquinone, a superoxide dismutaseand an iron sulfur protein have been...
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Báo cáo khoa học: Bacterial IscU is a well folded and functional single domain protein pdf
... Bacterial IscU is a well folded and functional single domain proteinSalvatore Adinolfi1, Francesca Rizzo1, Laura Masino1, Margie Nair1, Stephen R. Martin1, Annalisa Pastore1and ... occurring. In all datasets, the absorbance of the depleted area at a finalspeed of 40 000 r.p.m. provided an experimental value for the baseline offset. The data were analysed with the ORIGINXL- A /XL1 ... by the solvent is observed.Binding of a paramagnetic species leads instead to majorFig. 4. Probing the aggregation state of IscU. (Top) Calibration curvefor apparent molecular mass determination...
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