Báo cáo khoa học: Interaction analysis of the heterotrimer formed by the phosphatase 2A catalytic subunit, a4 and the mammalian ortholog of yeast Tip41 (TIPRL) ppt
... FEBS
Interaction analysis of the heterotrimer formed by the
phosphatase 2A catalytic subunit, a4 and the mammalian
ortholog of yeast Tip41 (TIPRL)
Juliana H. C. Smetana and Nilson I. T. Zanchin
Center for ... the interaction of TIPRL with the
C-terminal region of the catalytic subunits of type 2A
phosphatases. TIPRL forms a heter...
... illustrated
by the superimposition of the 3D structures of kistrin
[32] and cardosin A (Fig. 8). The importance of both
motifs and in particular their basic residues was fur-
ther emphasized by the complete ... at the
tip of a loop protruding away from the core of the
protein. This amino-acid motif mimics RGD in terms
of charge and is positioned at the ti...
... gifts
of the MAP2 cDNA and the pCAG expression vector,
respectively. The present study was supported by
grants from the Japanese Ministry of Education,
Culture, Sports, Science and Technology; the ... 1657
Discussion
The present study revealed the structural and func-
tional determinants of the protein–protein interaction
between v-KIND and MAP2 (i.e. the M...
... depends on
the relative amplitude and rate constant of the J–I
phase compared to the amplitudes and rate constants
of the two other phases. The above should be gov-
erned by the balance between the ... modeled by
the superposition of the exponential rise to analyze
quantitatively the contribution of each phase. The
amplitude and rate constant of each...
... (Met1)-ONC
(M23L) by the addition of AAP, as described in the
Materials and methods. Completion of the hydrolysis was
confirmed by the disappearance of the (Met1)-ONC
(M23L) signal in the MALDI-TOF mass ... analyzed by MALDI-TOF MS. The
addition of the protease is considered the zero time point.
The relative intensities of the peaks from the mass spect...
... DEC1 with BMAL1 and the bin ding to the E-box.
Substitution of Arg65 for Ala abolished the interaction of
DEC1 with BMAL1 and the binding of DEC1 to the E-box.
However, substitution of His57 for ... regulating the
mammalian circadian rhythm by suppressing the
CLOCK/BMAL1-activated promoter. The interaction of
DEC1 with BMAL1 has been suggested a s one of...
... show that the data fit well with the indi-
vidual determination of flux control coefficients by
PFK, PK and LDH. The role of the las operon is dis-
cussed on the basis of the distribution of flux control
for ... individually and simultaneous by changing the
expression of the respective genes. We found that nei-
ther the individual enzymes nor the sum of th...
... bond, linkage of the active
site Ser of the protease to the carboxyl group of P
1
by an
ester bond and insertion of the N-terminal part of the RCL
as strand 4 in b-sheet A (s4A) of the serpin. ... behaviour of the serpin.
Complex formation between serpins and their cognate
proteases is fuelled by the thermodynamic properties of the
serpin. Accordingly...
... appearance of an additional peak at 7.65 mL
on the elution profile. Increase of the temperature of
incubation was accompanied by the simultaneous increase
of the peak eluted at 7.65 mL and decrease of the ... 0.05, and 0.15 ± 0.06 l
M
in the absence of HSP25,
and in the presence of the wild type HSP25 and its 3D
mutant, respectively. At the same time,...
... spectroscopy. The intensity of
Trp fluorescence of purified HpPex5p increased by 25%
upon shifting the pH from pH 6.0 to pH 7.2. Together with
the results of fluorescence quenching by acrylamide, these
data ... calculation of the fraction (f
a
) and the effective
quenching constant (K
Q
) for the most accessible class of
Trp chromophores [16].
The pH dependence of...