Báo cáo khoa học: Interaction analysis of the heterotrimer formed by the phosphatase 2A catalytic subunit, a4 and the mammalian ortholog of yeast Tip41 (TIPRL) ppt

Báo cáo khoa học: Interaction analysis of the heterotrimer formed by the phosphatase 2A catalytic subunit, a4 and the mammalian ortholog of yeast Tip41 (TIPRL) ppt

Báo cáo khoa học: Interaction analysis of the heterotrimer formed by the phosphatase 2A catalytic subunit, a4 and the mammalian ortholog of yeast Tip41 (TIPRL) ppt

... FEBS Interaction analysis of the heterotrimer formed by the phosphatase 2A catalytic subunit, a4 and the mammalian ortholog of yeast Tip41 (TIPRL) Juliana H. C. Smetana and Nilson I. T. Zanchin Center for ... the interaction of TIPRL with the C-terminal region of the catalytic subunits of type 2A phosphatases. TIPRL forms a heter...
Ngày tải lên : 07/03/2014, 05:20
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Báo cáo khoa học: Molecular analysis of the interaction between cardosin A and phospholipase Da Identification of RGD/KGE sequences as binding motifs for C2 domains pdf

Báo cáo khoa học: Molecular analysis of the interaction between cardosin A and phospholipase Da Identification of RGD/KGE sequences as binding motifs for C2 domains pdf

... illustrated by the superimposition of the 3D structures of kistrin [32] and cardosin A (Fig. 8). The importance of both motifs and in particular their basic residues was fur- ther emphasized by the complete ... at the tip of a loop protruding away from the core of the protein. This amino-acid motif mimics RGD in terms of charge and is positioned at the ti...
Ngày tải lên : 30/03/2014, 11:20
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Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx

Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx

... gifts of the MAP2 cDNA and the pCAG expression vector, respectively. The present study was supported by grants from the Japanese Ministry of Education, Culture, Sports, Science and Technology; the ... 1657 Discussion The present study revealed the structural and func- tional determinants of the protein–protein interaction between v-KIND and MAP2 (i.e. the M...
Ngày tải lên : 14/02/2014, 19:20
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Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll fluorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc

Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll fluorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc

... depends on the relative amplitude and rate constant of the J–I phase compared to the amplitudes and rate constants of the two other phases. The above should be gov- erned by the balance between the ... modeled by the superposition of the exponential rise to analyze quantitatively the contribution of each phase. The amplitude and rate constant of each...
Ngày tải lên : 19/02/2014, 05:20
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Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf

Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf

... (Met1)-ONC (M23L) by the addition of AAP, as described in the Materials and methods. Completion of the hydrolysis was confirmed by the disappearance of the (Met1)-ONC (M23L) signal in the MALDI-TOF mass ... analyzed by MALDI-TOF MS. The addition of the protease is considered the zero time point. The relative intensities of the peaks from the mass spect...
Ngày tải lên : 19/02/2014, 12:20
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Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... DEC1 with BMAL1 and the bin ding to the E-box. Substitution of Arg65 for Ala abolished the interaction of DEC1 with BMAL1 and the binding of DEC1 to the E-box. However, substitution of His57 for ... regulating the mammalian circadian rhythm by suppressing the CLOCK/BMAL1-activated promoter. The interaction of DEC1 with BMAL1 has been suggested a s one of...
Ngày tải lên : 19/02/2014, 16:20
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Tài liệu Báo cáo khoa học: Control analysis as a tool to understand the formation of the las operon in Lactococcus lactis doc

Tài liệu Báo cáo khoa học: Control analysis as a tool to understand the formation of the las operon in Lactococcus lactis doc

... show that the data fit well with the indi- vidual determination of flux control coefficients by PFK, PK and LDH. The role of the las operon is dis- cussed on the basis of the distribution of flux control for ... individually and simultaneous by changing the expression of the respective genes. We found that nei- ther the individual enzymes nor the sum of th...
Ngày tải lên : 19/02/2014, 17:20
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Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf

Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf

... bond, linkage of the active site Ser of the protease to the carboxyl group of P 1 by an ester bond and insertion of the N-terminal part of the RCL as strand 4 in b-sheet A (s4A) of the serpin. ... behaviour of the serpin. Complex formation between serpins and their cognate proteases is fuelled by the thermodynamic properties of the serpin. Accordingly...
Ngày tải lên : 20/02/2014, 11:20
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Tài liệu Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin doc

Tài liệu Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin doc

... appearance of an additional peak at 7.65 mL on the elution profile. Increase of the temperature of incubation was accompanied by the simultaneous increase of the peak eluted at 7.65 mL and decrease of the ... 0.05, and 0.15 ± 0.06 l M in the absence of HSP25, and in the presence of the wild type HSP25 and its 3D mutant, respectively. At the same time,...
Ngày tải lên : 20/02/2014, 23:20
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Tài liệu Báo cáo khoa học: Fluorescence analysis of the Hansenula polymorpha peroxisomal targeting signal-1 receptor, Pex5p pdf

Tài liệu Báo cáo khoa học: Fluorescence analysis of the Hansenula polymorpha peroxisomal targeting signal-1 receptor, Pex5p pdf

... spectroscopy. The intensity of Trp fluorescence of purified HpPex5p increased by 25% upon shifting the pH from pH 6.0 to pH 7.2. Together with the results of fluorescence quenching by acrylamide, these data ... calculation of the fraction (f a ) and the effective quenching constant (K Q ) for the most accessible class of Trp chromophores [16]. The pH dependence of...
Ngày tải lên : 21/02/2014, 00:20
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