... intrabacterial cAMP and cGMP
concentrations
The levels of cAMP and cGMP in bacteria exposed to
natriuretic peptides were determined using cAMP and a
W. Veron et al. Effect of natriuretic peptides on Pseudomonas
FEBS ... 253–263.
Effect of natriuretic peptides on Pseudomonas W. Veron et al.
5864 FEBS Journal 274 (2007) 5852–5864 ª 2007 The Authors Journal compilation ª 20...
... (G-CSF) and cortisol.
Peptides were solubilized in acidi®ed chloroform/methanol,
and partitioned in chloroform/methanol/water. Water-
soluble polypeptides were separated by cation-exchange and
reversed-phase ... antibacterial peptides; histone; post-translational
modi®cation; ubiquitination; tandem mass spectrometry.
In innate immunity, antibacterial peptides are important
effector...
... Maaheimo
3
,
Pirkko Mattila
2
and Risto Renkonen
1
1
Department of Bacteriology and Immunology, Haartman Institute and Biomedicum, University of Helsinki, Finland;
2
MediCel, Helsinki, Finland;
3
VTT Biotechnology, ... rhamnosylation of g lycoprotein a nd glyco-
peptides.
Keywords: A-band O-antigen; GDP-4-keto-6-deoxy-
D
-mannose r eductase(RMD);GDP-rhamnose; Pseudomonas
aeruginosa...
... of
chlorophyll biosynthesis
Mark Shepherd, Samantha McLean and C. Neil Hunter
Robert Hill Institute for Photosynthesis and Krebs Institute for Biomolecular Research, Department of Molecular Biology and ... product and intermediate
evolution. All solutions contained 100 m
M Tris pH 7.5, and 100 mM
NaCl. Immediately after mixing, concentrations of ChlM, SAM and
MgD were fixed at 0....
... polymerase and
2 lL of the template library in a final volume of 20 lL.
The amplified enolase PCR product and the pQE30
plasmid vector were digested with KpnIandPstI
restriction enzymes, and these ... were screened by PCR and plasmid DNA
preparation, and finally sequencing was performed (Mac-
rogen Inc., Seoul, South Korea) using standard protocols
[20].
Expression in
E. coli
and pre...
...
integrates local and global coherence concerns.
Summary sentences are grouped before
ordering is applied on two levels: group-level
and sentence-level. Different algorithms for
grouping and ordering ... identification, interpretation, and
summary generation (Hovy, 2005). In the simple
case of extraction, topic identification and
interpretation are conflated to sentence select...
... from lobster and Saccharomyces cerevisiae, both
as apoenzyme structures and complexes with substrates
and inhibitors [4–7]. These two enolases have very similar
amino-acid sequences and three-dimensional ... developmentally and tissue
specifically, but the kinetic properties of all isoenzymes are
very similar [14,15]. In yeast, the levels of the two
isoenzymes are under metabolic a...
... bacteria,
fungi and protozoa: making it and taking it. Microbiol-
ogy 155, 1386–1396.
88 Gardocki ME, Jani N & Lopes JM (2005) Phosphati-
dylinositol biosynthesis: biochemistry and regulation.
Biochim ... PC
and diacyl PE species and a small increase in alk-1-enyl-acyl PE. Changes in the PE and PC contents in (B) and (C) relative to control cells (A)
are reflected by the siz...
... tetraphenylarso-
nium and ethidium bromide and it decreases the resistance
against divalent cations like dequalinium and methyl
viologen [13]. A region including TMS 2 to 5, and
homologous to proteins ... spectro-
scopy and by functional assays.
Experimental procedures
5
Strains, plasmids, oligonucleotides and DNA techniques
6
Strains and plasmids used in this study are listed i...