... 4865
Colocalization of annexins and dicalcin in frog
olfactory and respiratory epithelium
Dicalcin has been reported to localize in the cilia of
frog olfactory and respiratory epithelium [7]. To
understand the ... of dicalcin with annexin A1, annexin A2 or annexin A5 in frog olfactory epithelium. (A–I) Immunofluorescence double-
staining of dica...
... family of around 20 distinct enzymes involved in
maintaining the fidelity of protein synthesis through
the specific esterification of an amino acid to the 2¢-or
3¢-hydroxyl group of the 3¢-terminal ... min
3.5 4.0 4.5 5.0 5.5 6.0
90 min
63 min
36 min
9 min
t / min
Fig. 1. (A) Ion exchange HPLC measurement of mononucleotide and dinucleotide levels observed in a LysU catalysis mixt...
... experimentally
the determinant of V
H
⁄ V
L
interaction strength or its
relationship with antigen-binding affinity. Previously,
the effect of mutations of the V
H
⁄ V
L
interface residues
on antigen binding has ... affinity
and the V
H
⁄ V
L
interaction strength determined, five
clones in the H39Q group showed both high antigen-
binding affinity and strong V
H
⁄ V
L
interaction...
... compared with that of the scaffolding domain,
in repeated experiments.
Structure of ET
B
R recognized by caveolin
Since caveolin interacts with ET
B
R via the scaffolding and
C-terminal domains, ... suggest that caveolin-1 also utilizes the scaffolding
and C-terminal domains to interact with ET
B
R, as with
other signalling molecules. The binding of the C-terminal
domain f...
... solution
containing the protein. The binding constant (K
b
) was
determined using the simplest binding model, assuming that
one molecule of ligand binds to one molecule of protein.
The molar ... under-
stand the molecular basis of the interaction between
SlyD and HypB, and this could shed light on the role
of the atypical nickel ion-binding PPIase domain of
SlyD in the hydro...
... Nonspecific binding was measured as the binding
of
125
I-labelled toxin in the presence of an excess of unlabe-
led LT-B.
To assay nonspecific binding of
125
I-labelled LT-I or
competitive inhibition ... B
subunits of toxin were incubated with cells for 30 min at
4 °C and then further incubated with
125
I-labelled LT-I for
60 min at 4 °C. The blocking effect of
125
I-labelle...
... hydrogen
bond with FAD [16], the model structures indicated
that it is also involved in interactions with the neigh-
bouring monomer. As shown in Fig. 7B, Tyr377 and
Asn379 in the loop region interact with ... consists of 10 amino acids,
seven of which are conserved within the species
(Fig. 2C) and two of which are conserved within all
ACAD family of enzymes, forming a...
... was minimized in two
stages. In the first stage, we kept the protein fixed and only
minimized the position of the water molecules and ions. In
A. Viegas et al. Determinants of ligand specificity in ... for screening and identifying ligand binding to
protein receptors. Angewandte Chemie-International Edi-
tion 42, 864–890.
16 Mayer M & Meyer B (1999) Characterization of li...
... in ammatory cytokines, including IL-1b, TNF-a and
IFN-c. (B) Chemokines expressed in the joint recruit leukocytes into
the joints. In addition to functioning in cell trafficking, several
chemokines have ... associated
mainly with in ltrating macrophage-like cells and
fibroblast-like cells in the RA synovium, and the inter-
action of activated leukocytes with FLS results i...
... that the N-terminal domain is involved in
protein–protein interactions with other hydrophilic domains
of neighboring integral membrane subunits, and interspecies
interactions are incompatible.
From ... [15–18]. In the
bovine complex a large subdomain is made up of 20
integral membrane proteins contributing > 60 transmem-
brane segments. Some of these must be intimately invo...