... crotonyl-CoA,
3-hydroxybutyryl-CoA and 3-methylcrotonyl-CoA
were used for the kinetic characterization of AUH.
The data are summarized in Table 2.
Overexpression of a mutant form of AUH and
its activity on (E)-3-MG-1-CoA
Mutations ... shoulder and the tailing is due to acetyl-CoA and not due to
any other compound. (B) The peaks 3, 4, and 5 were isolated by HPLC and used...
... large number of orthologs
(Figs 2,9). This suggests an essential role for FMN despite
the low affinity of the enzyme for that cofactor and the
apparent absence of a redox process as part of the catalytic
cycle. ... homooctamer
structure with a relative mass of 309 kDa [29]. In order to
check for the potential influence of substrates and cofactors
on the quaternary structur...
... enzyme
forms [16,17]. Even though S. cerevisiae is a close rel-
ative of Candida species, and is often used as a model
pathogen, its DHODH of family 1 is unsuitable as a
prototype for the search for ... calculated:
pK
a1
, 6.7 ± 0.05 for both enzymes; pK
a2
, 9.5 ± 0.1
for CaDHODH and 9.9 ± 0.15 for DNCaDHODH.
We compared the activity of CaDHODH and
DNCaDHODH with a vari...
... Yusof
1
, Erning Gao
1
, Hong-Li Yan
2
and Andreas Hofmann
1
1 Institute of Structural & Molecular Biology, School of Biological Sciences, The University of Edinburgh, UK
2 Department of ... precipitation
assay
Calcium-induced precipitation of annexins B1 and A1 was
performed as a control for the heparin binding and lipo-
some copelleting assays. 10 lLof2mgÆmL
)1
prote...
... domains that are responsible for binding
substrate and NADP cofactor. The detailed structural
information might expedite the discovery of novel
SDH inhibitors and further of antimicrobial agents,
though ... expressed,
and purified in Escherichia coli system. The enzymatic characterization of
HpSDH demonstrates its activity with k
cat
of 7.7 s
)1
and K
m
of 0.148 mm
to...
... in the 3¢ UTR. In recent years, and as a result
of great efforts aiming to understand the mechanism
of RNA control in animals, the function of a diverse
number of RNA-binding proteins has been ... that of other well-
characterized members of PUF family. These proteins
must bind to transcripts in the plant and regulate their
translation and ⁄ or turnover.
A large number of...
... (1971) The kinetics
and mechanisms of the reactions of iron(III) with b-dik-
etones. The formation of monoacetylacetoatoiron(III)
and the effect of copper (II) on the formation of
monothenoyltrifluoroacetonatoiron ... measurements of con-
sumption of O
2
and substrate with the formation of
detectable products. Figure S4 shows that conversion
of 2,4-pentanedione (5 mm)...
... at 60 °C for 5 min and then slow cooled to
44 °C before addition of the substrate, which was also at
44 °C. The chase phase was then initiated by removing
5 lL of the reaction mixture and diluting ... lLof8nm 5¢-end labeled sub-
strate and allowed to react in a total volume of 6 lL. The
ribozyme solution was preincubated at 60 °C for 5 min and
then slow cooled to 44 °C befo...
... Soret band maxima of the azide-free
form at 405 nm and of the azide-bound form at
421 nm, so the relative amounts of the nonbound and
azide-bound forms were estimated directly from the
values of ... with time, indicating the formation of biliverdin.
In this case, neither bands of the oxy form (at 541 and
578 nm) nor bands of verdoheme and CO–verdoheme
(at 690 and at...