Báo cáo khoa học: Phosphorylation of the Saccharomyces cerevisiae Grx4p glutaredoxin by the Bud32p kinase unveils a novel signaling pathway involving Sch9p, a yeast member of the Akt / PKB subfamily pot
... -ATC ATC TCC
ATC GAC TAC TCC CTG-3¢, the antisense primer
5¢-AAG AAT
TCT AGA TTA ATG GTG ATG ATG GTG
ATG ATG GTG TGG GGT CAG CGG TGC AGC AGG
GGG GGT-3¢ (XbaI sites underlined; His-tag in italic) ... activity by bis-ANS was
decreased upon phosphorylation. A possible explana-
tion for this apparent discrepancy could be that PKA
phosphorylation induces a conformational change that
inc...
... McIntosh EM & Haynes RH (1984) Isolation of a
Saccharomyces cerevisiae mutant strain deficient in
deoxycytidylate deaminase activity and partial charac-
terization of the enzyme. J Bacteriol 158, ... Coordinated
closure of the active site and rearrangement of the
main chain and side chains in the active site appear
as key players in a slow transformation from an
ina...
... 3¢-GG
AACCACGAAAGTAG CAATC AGA ACACTA AAACC A
GGTACAATGATGC-5 ¢ . All vector inserts were
sequenced prior to use.
CDK4 pY17 antibody generation
Murex lop-eared rabbits were injected with a phosphopep-
tide ... C-YES and C-SRC were assayed for Y17 kinase activity
using the plate format assay at various concentrations of the Src
family kinase inhibitor PP2. Values are the averag...
... treated in the same way as described
above. The reaction was started by the addition of fumarate
instead of DMN, and the consumption of fumarate was
recorded in a se parate experiment in the absenc ... technical reasons, the H
+
/e ratio of apparent proton
translocation can only be measured at vanishing Dp.Itis
generally thought that the same ratio is valid in the...
... this was a valid assumption as loss of
area from the CoQ
2
H
2
and decylQH
2
peaks was compara-
ble with the gain in size of the CoQ
2
and decylQ peaks.
Determination of the fraction of CoQ
2
and ... library
A genetically stable double-knockout Dcoq2DORF MATa
library was created using the approach of Tong and
coworkers [14]. We utilized the commercially available dip...
... upregulation of both RGS
mRNA was found only after activation of the TLR3
signalling pathway. This upregulation of RGS1 and
RGS2 mRNA is due to the TRIF pathway. We veri-
fied the data by stimulation ... stimu-
lates the GTPase activity of several members of the
Ga
i
subfamily but is ineffective against Ga
s
[21],
whereas RGS2 does not interact with Ga
i
,Ga
o
,Ga
s
a...
... counteracting the
glycosylation pattern associated to malignancy.
We found in fact that NeuAca2-3Galb1-3[Fuca1-4]Glc-
NAcb1-3Gal and NeuAca2-3Galb1-3GlcNAcb1-3Gal are
the main oligosaccharides released by ... b1,4- and b1,3galactosidases,
giving rise to a disaccharide and a monosaccharide, and
was thus identified as a mixture of Galb1-4[Fuca1-3]Glc-
NAcb1-3Gal and Galb1-3[Fuca1-4...
... 5 ¢-GCAACACCATGGCTTCTTACAAAG
TGAAA-3¢ and Fdlw 5¢-CCACAAGCTTGATATCATA
TCATAGCATAGCAGT-3¢ and the full length p ea Fd
precursor cDNA as template. To facilitate the cloning
process, NcoIandHindIII ... Fd-NADP
+
reductase was purified according
to published procedures [32].
Fld from Anabaena was k indly provided by M. Medina
(University of Zaragoza, Zaragoza, Spain). ApoFld from
Anabaena...
... [40].
Two major pathways for caspase activation have
been identified: the receptor pathway and the mito-
chondrial pathway; both pathways trigger a cascade of
downstream caspases that induces DNA fragmentation
[2]. ... mitochondrial pathway [47]. Caspase-9 is also
cleaved by caspase-3 at another cleavage site. How-
ever, this fragmentation does not have caspase activity.
It enha...
... indicated strains
were grown to stationary phase and then lipids were extracted and
separated by TLC. The amount of SE of the wildtype strain was set
at 100%. Data are mean values of three independent ... substratum [10].
Among the Cdc42p effectors that regulate cell polari-
zation are Ste20p and Cla4p, both members of the p21-
activated kinase (PAK) family [4,11]. Cla4p p...