... 540 8–5 421 ª 2008 The Authors Journal compilation ª 2008 FEBS 5421
ATP-dependent ligases in trypanothione
biosynthesis – kinetics of catalysis and inhibition
by phosphinic acid pseudopeptides
Sandra ... concentration of
phosphinate and initiating with enzyme.
Modality of inhibition
The mode of inhibition of the slow-binding phosphi-
nate was de...
... procedures
Cloning and expression of the open reading
frames for gld1 and gld2
The gld1 gene was cloned from a cDNA library of the
H. jecorina strain Rut C-30 [21] by PCR. The following
primers, introducing ... The GLD1 pro-
tein was tagged with a histidine tag at the N-terminal
end by adding the coding sequence for six histidines to
the end of the open reading frame, and then...
... proteins
Steffen P. Graether and Brian D. Sykes
CIHR Group in Protein Structure and Function, Department of Biochemistry and Protein Engineering Network of Centres of
Excellence, University of ... protein and i ce at the atomic level and
has included the determination of a number of fi sh AFP
structures (Fig. 1 ) (reviews in [ 9–1 6]). Early models of the
interact...
... obtained from different clones of the
same cell line, or among data obtained in single controls
and in single cell lines affected by the same mtDNA
mutation, we decided to carry out the following
experiments ... blotting analysis of content of CuZnSOD (16 kDa)
and MnSOD (25 kDa) proteins in control and LHON-affected cybrid
cells maintained in glucose-supplemented culture...
... original because of the writer’s relative lack of ex-
pertness in the Russian language) and sorted by the
writer into nine piles of ascending degrees of “informa-
tiveness” of the original ... hundred sentences, consisting of nearly all the
translations of the sentences in Passage 1, were sorted
and re-sorted by the writer into nine piles of increasing
intelli...
... mechanism to increase the complexity of
proteomes is the editing of transcripts (both in nuclear
and organelle genomes) [4,5]. Dual targeting of
proteins does not increase the number of proteins in a
cell, ... targeting may occur. Finally, an outline
of the future challenges in this field is provided, and
the insights that may be gained from a greater under-
standing of t...
... residues in the N-domain of ClpA by monitor-
ing the binding, unfolding and degradation of several different substrates,
including short unstructured peptides, tagged and untagged proteins. Inter-
estingly, ... for
various adaptor proteins [ 6–1 0]. ClpA consists of three
domains: an N-domain and two ATP-binding domains
referred to as the D1 and D2 domains. Interestingly,...
... continue to discuss the
solution of these first two types, since it will
be contained in that of the main problem, the
distinction of nominative from accusative in the
feminine and neuter singular ... syntactical function of such
forms is ambiguous, the types of ambiguity be-
ing three in number.
1. The masculine singular nominative of der
and welcher is identical...
... to obtain complementary information concerning
protein–solvent interactions [14,15], the unfolded states of
proteins [16,17], and protein folding pathways [18].
Our analytical techniques involved ... (propeptide) of 91 residues [2,3],
compared to the mature CPY. This propeptide structure is
essential for folding both in vivo and in vitro,aswellasfor
maintaining CPY in an inactiv...
... G-protein
interaction of the active receptor [28].
In summary, Arg46 of the V
1a
R is required for high
affinity agonist binding and signalling. It is one of the
constraining residues which maintain ... arginyl at this locus is a constraining
residue which contributes to maintaining the conformational
switch of the V
1a
R in the off-state. The unique ability of
arginyl to fulfil...