... abundant enzymes in the biosphere [1,2]. It
consists of the Fe-protein (dinitrogenase reductase,
also referred to as NifH), an a
2
dimer of the nifH gene
product and of the MoFe-protein (dinitrogenase) ... suggested
that nitrogenase Fe-protein was modified by ADP-
ribosylation of R102 also in Azoarcus sp. BH72.
Further support was obtained by site-directed muta-...
... thus the presence of
the N-terminal methionine may enable detection of
Asp1 that would otherwise be invisible in
1
H
15
N-
HSQC spectra. Therefore, the presence of the N-termi-
nal methionine ... chromatogram obtained with UV absorption at 214 nm, the
middle panel is the corresponding total ion-current after infusion into the mass spectrometer, and the bottom panel i...
... in insulin-secreting b-cells. In order to gain
more insight into the physiological role of mir-9 in
insulin secretion, we have specifically addressed the
link between GSIS and the regulation of ... conditions, there was a dramatic
effect on insulin secretion in response to glucose chal-
lenge (GSIS). These reports strongly suggest a role for
Sirt1 in the regulation of i...
... K, Hoffmann S, Bauer F, Friedrich U,
Kardinal C, Feller SM, Biesinger B & Sticht H (2002)
Structural investigation of the binding of a herpesviral
protein to the SH3 domain of tyrosine kinase ... CD4 in the endoplasmic reticulum, which is
mediated by the cytoplasmic region of the protein [1,2].
This function involves binding and recruitment of the
b-transducin repe...
... Taken together with the inability of P III
and P IV to inhibit hemagglutination in vitro in the
presence of this sugar, these data suggest that the bind-
ing and uptake of these two isoforms does ... one. In the four pulchellin A-chains,
the residues involved in the active site cleft are the
same as in abrin and ricin A-chains. This suggests that
the catalytic...
... I (NADH:ubiquinone oxidoreductase),
the largest and most complicated of these, is the main
point of entry into the respiratory chain for electrons.
The mammalian complex I contains nearly 50 ... catalyzes the
oxidation of succinate to fumarate in the mitochond-
rial matrix as succinate dehydrogenase. Succinate oxi-
dation is coupled to reduction of ubiquinone to
ubiquino...
... Da). The mass of the follow-
ing theoretical tryptic peptide (SSVTTIINQLYGP-
NSGK, m ¼ 1777.927 Da) could not be found,
indicating that the C-terminus of the protein is within
this sequence. In the ... [16]. The met state is the resting state of the
enzyme, and in the absence of substrate about 85–90%
of the enzyme is in this state [16]. Both the met an...
... deletion in the autoinhibitory
(NAF) motif failed to interact with PsCBL thus con-
firming the authenticity of these proteins and emphasi-
zing the importance NAF in the interaction between
them (data ... is
the first indication showing the phosphorylation of a
CBL by its interacting kinase. An understanding of the
functional significance of this observation and the
e...
... DNA-binding domain of HSF, whereas clone
C2 contained an insert of 2771 nucleotides including introns
and an ORF encoding 513 amino acids.
By using 5¢-and3¢-RACE, the full-length cDNAs of
clones ... clarify the molecular
mechanism underlying the stress response in cold-water fish
species. Here, we present evidence for existence of two
distinct HSF1 isoforms in rainbow trout a...
... by mass spectrometry and
the lability of the sulfogroup in mass spectrometry
analysis allows recognition of the modification, and
differentiation of sulfotyrosine and phosphotyrosine [38].
Characterization ... the sulfated peptides [21,35]. In
the case of AnIB, tyrosine sulfation selectively in uenced the
binding to the mammalian a7 but not the a3b2 subtype...