... polyQ
and polyQ-expanded Huntingtin formed immobile
inclusions in the cytosol and in the nucleus [20]. Thus,
there are at least two types of inclusions – mobile inclu-
sions and immobile inclusions ... glutamine residues in healthy individuals,
whereas they are expanded to more than 40 repeats in
patients.
In the polyQ diseases, inclusions containing polyQ-
expanded...
... differentiation
was initiated at 48 h post-transfection by maintaining the cells in culture medium containing 2% horse serum. Cells were fixed on day 4 of
differentiation and stained with hematoxylin and eosin. ... control was maintained in a standard incubator with 21% O
2
. The normoxia data are the same as shown in
Fig. 1 and are included here for comparison. Expression of...
... M (2004)
Kinetics and binding sites for interaction of the prefol-
din with a group II chaperonin: contiguous non-native
substrate and chaperonin binding sites in the archaeal
prefoldin. J Biol ... death and blocked LTP.
The finding of ADDL in soluble brain extracts from
the human AD brain using ADDL-specific antibody
supports the idea that the existence of ADDLs in the...
... this context, the amyloids that are used in the
prion should be fragile. Understanding, in greater
detail, the intrinsic fragility of the amyloids and their
susceptibility to trans-acting factors, ... in the cells after the foci disappeared.
The live-cell imaging showed the appearance of the
foci in the daughter cell at almost same time as when
the foci re-appe...
... structure. The situation is, in some
respects, similar to the situation of studying protein
folding and dynamic protein–protein interactions, such
as in the case of chaperone action. Restricting us ... twofold:
the necessity of a cysteine-scanning approach; and the
intrinsic bulkiness and hydrophobicity of the pyrene
label.
Modern fluorescent techniques and
novel spectr...
... nuclei within the same cytoplasm. One is the
germinal micronucleus and the other is the somatic
macronucleus. These nuclei both originate from a ferti-
lized micronucleus (synkaryon) during conjugation
[14,15]. ... caspase-8- and caspase-9-
like activities, which appear to be involved in the final
resorption of the parental macronucleus during PND
in T. thermophila, and s...
... Italy
Introduction
Towards the end of the 1970s, in the beginning of
pre-mRNA splicing research [1,2], defining exons and
introns was essentially based on observing the final
composition of the mature ... J (2006) A splice-
supporting intronic mutation in the last bp position of
a cryptic exon within intron 6 of the CYBB gene
induces its incorporation into the mRNA causing...
... lack of DNA binding. Indeed,
in the case of War1-42p, in vivo footprinting data
(Fig. 5) indicate an inability to bind to the WARE,
which is normally decorated by wild-type War1p in the
presence ... expressed and
properly localized in the nucleus, the War1-42p variant fails to bind the
weak-acid-response elements in the PDR12 promoter, as shown by in vivo
footprin...
... negative
when the n-gram occurs more in the test data than
in the training data. Let W
i
= {w : k
train
(w) = i}
be the set of n-grams with count i in the training
corpus. We define the average ... configurations, training on NYT95 and testing on the
indicated corpora. For each n-gram count k, we compute
the average number of occurrences in test for all n-grams
occurri...
... representation /an-
guages in order to focus on formally specifying
domains and using ~hat specification in language
processing rather than on defining new domain-
independent representation ... supporting
this hypothesis appear in [2, 13] for interpreting
nominal compounds; in [6, 7, 29], for common
sense reasoning about modifier attachment;
and in [32] for phenomena in...