Báo cáo khoa học: Starch-binding domains in the CBM45 family

Báo cáo khoa học: Secondary transporters of the 2HCT family contain two homologous domains with inverted membrane topology and trans re-entrant loops docx

... shows the same analysis for the hits between the N- and C-terminal halves and vice versa within the OPA family, the AAT family, the ST3 families and the DAACS1 family. In case of the AAT and DAACS1 families, ... the sequences in the database is about 60%. Hits between the N- and C-terminal halves Each of the sequences in the domain database was use...

Ngày tải lên : 07/03/2014, 17:20

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Báo cáo khoa học: Catalytic mechanism of the primary human prostaglandin F2asynthase, aldo-keto reductase 1B1 – prostaglandin D2 synthase activity in the absence of NADP(H) pptx

... core in this family [ 3–6 ]. To iden- tify the catalytic residues involved in the PGDS and PGFS activity catalyzed by AKR1B1, we introduced site-directed mutagenesis into the tetrad, generating the D43N, ... b-barrel, with the nicotinamide ring projecting down into the center of the barrel and pyrophosphate straddling the barrel lip [7]. Kubiseski et al. [8] have...

Ngày tải lên : 14/03/2014, 23:20

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Báo cáo khoa học: New members of the brachyurins family in lobster include a trypsin-like enzyme with amino acid substitutions in the substrate-binding pocket potx

... different in comparison with those of vertebrate trypsins, and also they are important for inhibitor binding [8]. The phenylalanine and Ile residues in crayfish Loop37 interact with the C-terminal segment ... (216 and 226, chymotrypsin numbering) in bovine and all other crustacean trypsins; (B) predicted disulfide bridges in lobster trypsins; (C) calcium-binding site config...

Ngày tải lên : 23/03/2014, 03:20

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Tài liệu Báo cáo khoa học: DNA modiﬁcation with cisplatin affects sequence-speciﬁc DNA binding of p53 and p73 proteins in a target site-dependent manner pptx

... different intensities of the R bands). To eliminate these differences, the effects of DNA cis-platination on the protein–DNA interactions were always normalized with the intensity of the retarded band ... accommodate the cisplatin IACs. The higher the probability of formation of the cisplatin IACs within the p53DBSs due to the occurrence of the GG, AG and ⁄ or GNG...

Ngày tải lên : 19/02/2014, 05:20

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Báo cáo khoa học: Functional similarities between the small heat shock proteins Mycobacterium tuberculosis HSP 16.3 and human aB-crystallin docx

... containing aB-crystallin induced and not induced with IPTG. Lane 2–5 show aB-cr ystallin uninduced at the 0, 3 and 6 h time points. Lane 7 contains cell homogenates of aB-crystallin n ot induced. ... expression is increased in response to stress, it protects against protein a ggregation in v itro, and it contains the core Ôa-crystallinÕ domain found in all sHsps. In this s...

Ngày tải lên : 23/03/2014, 21:21

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Báo cáo khoa học: Heme binding to the second, lower-afﬁnity site of the global iron regulator Irr from Rhizobium leguminosarum promotes oligomerization potx

... higher afﬁnity binding of ferric heme. Heme binding to proteins is commonly pH-dependent [2 6–2 8] and thus heme binding in both the oxidized and reduced states was investigated at higher pH. In the oxidized ... effect on the UV-visible or EPR spectra, clearly indicating that these residues are not directly involved in heme binding. Furthermore, low-spin heme binding t...

Ngày tải lên : 28/03/2014, 22:21

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Báo cáo khoa học: A dimer of the FeS cluster biosynthesis protein IscA from cyanobacteria binds a [2Fe2S] cluster between two protomers and transfers it to [2Fe2S] and [4Fe4S] apo proteins ppt

... of three domains. The N-terminal domain is very similar in sequence and size to IscU, the central domain is similar to a protein carrying a [2Fe2S] cluster known as Bfd and the C-terminal domain is ... cluster [12]. Other proteins which have been shown to be involved in FeS cluster biosynthesis are the two chaperones HscA and HscB. There are indications that HscA/B intera...

Ngày tải lên : 31/03/2014, 01:20

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Báo cáo khoa học: Heteromer formation of a long-chain prenyl diphosphate synthase from ﬁssion yeast Dps1 and budding yeast pptx

... Protein Standard were obtained from Invitrogen (Osaka, Japan). Strains and plasmids The strains and plasmids used in this study are shown in Table 1. The E. coli strain DH5a was used in the general construction ... with the Coq1 protein and the pHADPS1 plasmid that produces the HA-fused Dps1 protein were introduced into Sc. pombe strain LA1. Crude proteins extracted...

Ngày tải lên : 30/03/2014, 04:20

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Báo cáo khoa học: Starch-binding domains in the CBM45 family – low-afﬁnity domains from glucan, water dikinase and a-amylase involved in plastidial starch metabolism pptx

... 2011) doi:10.1111/j.1742-4658.2011.08043.x Starch- binding domains are noncatalytic carbohydrate-binding modules that mediate binding to granular starch. The starch- binding domains from the carbohydrate-binding module family 45 (CBM45, ... 1185 Starch- binding domains in the CBM45 family – low-afﬁnity domains from glucan, water dikinase and...

Ngày tải lên : 06/03/2014, 00:21

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Báo cáo khoa học: Respective roles of the catalytic domains and C-terminal tail peptides in the oligomerization and secretory trafﬁcking of human acetylcholinesterase and butyrylcholinesterase potx

... This indicates a predominant in uence of the catalytic domain on oligomerization. Role of the C-terminal cysteine Mutation of the cysteine located at )4 from the C-ter- minus to a serine in the ... Aa and Ab, as well as for Ba and Bb, indicating a predominant in uence of the catalytic domains. However, addition of a cysteine within the aromatic-rich segment of the...

Ngày tải lên : 07/03/2014, 03:20

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