... MINIREVIEW
Biological role of bacterial inclusion bodies: a model for
amyloid aggregation
Elena Garcı
´
a- Fruito
´
s
1–3,
*, Raimon Sabate
1,4,
*, Natalia S. de Groot
1,4
, Antonio Villaverde
1–3
and
Salvador ... Garcı
´
a- Fruito
´
s E, Gonzalez-Montalban N, Morell M,
Vera A, Ferraz RM, Aris A, Ventura S & Villaverde A
(2005) Aggregation as bacterial inc...
... Moskalenko AA & Karapetyan NV (1996) Structural
role of carotenoids in photosynthetic membranes.
Z Naturforsch 51c, 763–771.
24 Trebst A & Depka B (1997) Role of carotene in the
rapid ... isomers of
b-carotene, a monohydroxy carotenoid and zeaxanthin, respectively)
instead. This indicates a substantial role of the carotenoid – possibly for
strucure and assembly –...
... germ-cell development and germ-cell malignancy.
Nature 460, 909–913.
89 Hong H, Takahashi K, Ichisaka T, Aoi T, Kanagawa
O, Nakagawa M, Okita K & Yamanaka S (2009)
Suppression of induced pluripotent ... cells.
Exp Hematol 38, 809–818.
88 West JA, Viswanathan SR, Yabuuchi A, Cunniff K,
Takeuchi A, Park IH, Sero JE, Zhu H, Perez-Atayde
A, Frazier AL et al. (2009) A role for Lin28...
... 5¢-CCCGCCATGGGTAAAATAATTGGTA
TCG-3¢ and 5¢-CCCGGATCCAAGCTTTTACTGCTTAG
TTTCACCAGA-3¢. The PCR fragments were cloned into
the bacterial expression vector pTrc9 9A (Amersham Phar-
macia Biotech, Piscataway, NJ) following ... structure and specificity F. Moro et al.
Conformational properties of bacterial DnaK and yeast
mitochondrial Hsp70
Role of the divergent C-terminal a- helical sub...
... specifically
associate to negatively charged membranes.
As all these data are suggestive of a role of mem-
branes, in particular of negatively charged membranes,
in the mechanism of action of RNases, ... dimeri-
zation into RNase-AA.
The replacement in dimeric RNase-AA of one negat-
ively charged residue (Asp38) with an uncharged Gly
residue generates an RNase variant (RNase-AA...
... syndrome and adipocytokines
Y. Matzuzawa
Department of Internal Medicine and Molecular Science, Graduate
School of Medicine Osaka University, Osaka, Japan
Visceral fat accumulation plays crucial roles ... yeast mutants blocked at various sta-
ges of transport pathways are an invaluable method for
unravelling the molecular details of vacuolar and lysosomal traf-
fic. The activities o...
... The role of the ESSS protein in the assembly of a functional and stable
mammalian mitochondrial complex I (NADH-ubiquinone
oxidoreductase)
Prasanth Potluri, Nagendra Yadava and Immo E. ... from
Covance BabCo, anti-mouse and anti-rabbit secondary
antibodies from Bio-Rad Laboratories and Amersham
Pharmacia Biotech, respectively. Antibodies against the
Rieske protein, PSST, and 18 kDa we...
... protein
)1
Æmin
)1
.
For quantitative measurement of the activity of GABA-
uptake, an aliquot of the stable CHO transfectants was
used for GABA-uptake assay, and another aliquot was
used to determine the quantity ... GABA-uptake activity
For quantitative measurement of the specific activity
of GABA-uptake, an aliquot of the stable CHO trans-
fectants was used for the GABA-u...
... 5¢-GTTCAGGC
CAGCATCTGTGGTGGTACAATTG-3¢ (sense), 5¢ -CAAT
TGTACCACCACAGATGCTGGCCTGAAC-3¢ (antisense),
S ⁄ A substitution: 5¢-GTTCAGGCCAGGAGCTGTGGTG
GTACAATTG-3¢ (sense), 5¢-CAATTGTACCACCACAG
CTCCTGGCCTGAAC-3¢ (antisense), ... the
WT
F /A
R /A
U
G /A
V /A
S /A
50
75
105
160
250
U
WT
F /A
R /A
G /A
S /A
V /A
250
50
75
105
35
30
25
WT
F /A
R /A
G /A
S /A
V /A
WT
F /A
R /A
G /A...
... structural domain
that were sometimes missing in the Pfam alignments were
added manually. Sequence analysis was performed using
CLUSTAL X
software for multiple alignment and
SEAVIEW
software for manual ... manual adjustment. Three dimensional struc-
ture analysis and drawing were done with
SWISS
-
PDB VIEWER
v3.7 using the 1jfn file from the Protein Data Bank.
Antibacterial assays
Antibact...