Báo cáo khoa học: A novel mechanism of V-type zinc inhibition of glutamate dehydrogenase results from disruption of subunit interactions necessary for efficient catalysis doc
... Authors Journal compilation ª 2011 FEBS 3151
A novel mechanism of V-type zinc inhibition of glutamate
dehydrogenase results from disruption of subunit
interactions necessary for efficient catalysis
Jaclyn ... that the V-type inhibition produced with glutamate as the substrate
results from disruption of subunit interactions necessary...
... GCG
GGTACCAATGTGATGGGTGGACTGGT
hRhoA +166 GCG
AAGCTTACCAGACCGTGGACTAACGA
hRhoB sense CCCACCGTCTTCGAGAACTA
hRhoB
antisense
CTTCCTTGGTCTTGGCAGAG
hRhoA sense CCAGACTAGATGTAGTATTTTTTG
hRhoA
antisense
ATTAGAGCCAGATGCTTAAGTCC
GAPDH-F ... 3T3
fibroblasts. These data reveal a novel mechanism of cross-talk between
the classical TGFb ⁄ Smad pathway and Rho GTPases, regulating the rapid
and the l...
... (sense) and
5¢-GAAAAAACGCGATCCTACTT-3¢ (antisense). Primers
for unmethylated DNA were: 5¢-GAAGTAGGTGGAGT
ATTGAAT-3¢ (sense) and 5¢-CAAAAAAACACAATCCT
ACTT-3¢ (antisense).
Caspase 3 activity
Cells ... intensity was determined using imagemaster
2d elite software 4.01 (Amersham Bioscience, Uppsala,
Sweden).
Statistical analysis
Data in bar graphs are expressed as the mean and standard
deviation...
... tachykinins: a review. Zool Sci 5,
533–549.
7 Satake H, Ogasawara M, Kawada T, Masuda K, Aoy-
ama M, Minakata H, Chiba T, Metoki H, Satou Y &
Satoh N (2004) Tachykinin and tachykinin receptor of
an ascidian, ... vulgaris).
Biochem J 387, 85–91.
25 Kanda A, Takahashi T, Satake H & Minakata H (2006)
Molecular and functional characterization of a novel
gonadotropin-releasing-h...
... the data above allowed the identification
of the carbohydrate backbone from alkaline degradation of
the rough form LPS from P. stutzeri OX1.
Isolation, NMR and MS analyses of oligosaccharide 2
from ... Gram-negative bacteria, and their adaptability to
many different pollutants [1].
Pseudomonas stutzeri OX1 is a Gram-negative bacterium
isolated from the activated sludge of a...
... NADPH, and glutamate dehydrogenase
were from Wako Pure Chemicals (Osaka, Japan); meat
extract (Extract Ehlrich) w as from Kyokuto Seiyaku Kogyo
(Osaka, Japan); and pentafluorophenylhydrazine was ... only as a carbon source, but also as a nitrogen
source for g rowth of the assimilating bacteria. Deaminases,
which catalyze the release of ammonia, are a key enzyme in
the metab...
... the
biodegradation of a n a lmost unlimited spectrum of natural
and man-made organic compounds, among them the
tobacco alkaloid nicotine. Perhaps analysed in greatest
detail is the pathway of nicotine ... pair 5¢-GAC
CTGAGTAGAAATGGATCCCTGA TGGACAGG-3¢
and 5¢-GGAATGGCTCGAGGGATCATCACC-3¢ bear-
ing the restriction e nzyme recognition sites Bam HI and
XhoI, respectively. pAO1 DNA, isola...
... gamus, Molgula occidentalis
and Pyura vittata); 11 species of Cnidaria (Bartholo-
mea annulata, Budonosoma granulifera, Cassiopea
xamachana, Condylactys gigantea, Gorgonia ventalina,
Lebrunia ... screened for CPA activity
using AAFP as a substrate: four species of Mollusca
(Aplysia dactylomela, Aplysia juliana, Isognomun radia-
tus and Lima scabra); four species of Chordata (Pallu-...
... against a shifting target: a structural basis for
resistance to inhibitors in a variant of influenza virus
neuraminidase. Structure 6, 735–746.
13 Ely B & Pittard J (1979) Aromatic amino acid
biosynthesis: ... inhibitory
concentration was about 0.41 lm. The results indicate
that binding of ATB107 reduces the catalytic activity
of mIGPS, and that ATB107 is a high-affinity i...
... processes such as transport,
protein synthesis, catabolism and metabolism [1]. It
can also protect cells against reactive oxygen species
and help them maintain an adequate intracellular
redox status [2]. ... centri-
fuged at 1000 g for 1 min at room temperature to
remove cells and platelets [10]. Afterwards, 0.50 mL
of absolute alcohol was added to the plasma
with shaking. Plasma proteins...