Tài liệu Báo cáo Y học: Analyses of the CYP11B gene family in the guinea pig suggest the existence of a primordial CYP11B gene with aldosterone synthase activity docx
... decrease in the activity
for the 11b-hydroxylase, CYP11B1 . Intriguingly, however,
the 11b-hydroxylase activity of the aldosterone synthase
CYP11B2 was basically unaffected whereas the 18-hydroxy-
lation ... to
adrenodoxin when compared with CYP11B2 . CYP11B1
has a tryptophan at position 366 while all other proteins of
the CYP11B family including CYP11B...
... structural factor
for folding of domains involved in protein–protein and
protein–DNA interactions.
A large majority of the zinc-containing enzymes have a
single zinc site consisting of a combination ... Department of Physics, Takatsuki,
Osaka, Japan
AsystematicsearchforEscherichia coli proteins with the
zinc-binding activity was performed using the assay of
radioac...
... Chile;
2
Department of
Microbiology and Immunology, University of Saskatchewan, Saskatoon, Canada
Escherichia coli phosphoenolpyruvate (PEP) carboxykinase
catalyzes the decarboxylation of oxaloacetate and ... catalyzes the reversible decarb-
oxylation of oxaloacetic acid (OAA) with the associated
transfer of the c-phosphoryl group of ATP to yield PEP and
ADP, where M
2+...
... mutually
interacting binding sites containing an active-site cysteine
that is accessible from both faces of the membrane. The
nature of the structural rearrangement for this alternating
accessibility ... the other hand, the rate of
inactivation by bromoacetyl-Glc ( 1a) was 15 times faster
than by bromoacetic acid and 2.5 times faster than by the
chemically more reactive iodo...
... Dnmt1 is
controlled by interaction of the catalytic domain with the
N-terminal part of the enzyme leading to an allosteric activation of
the enzyme after binding to methylated DNA. J. Mol. Biol. ... additional DNA binding site in
its N-terminal part [33]. The observation that Dnmt 3a is not
activated by pre-exiting methylation agrees with the fact
that the rates of...
... detected against any of
the transgalactooligosaccharides listed in Materials and
methods.
Hydrolysis of arabinogalactans
The sugar composition of the arabinogalactans from
potato, onion and soy was ... galacto-oligosaccharides are detected.
These disappear in time, suggesting the presence of an
arabinose-releasing activity in the GALA preparation.
However, no a- 1-,...
... activity as described above, SOD
concentrations having been calculated to yield 1 U SOD
activity in a standard 1 mL assay. Activities were expressed
as a percentage of SOD activity at 25 °C without ... program
CE [35] while mutational analyses were carried out using
the CARA and ENCAD algorithms included in the
GENEMINE
program.
Bacterial strains and vectors
The mutage...
... the
terminal galactose and GalNAc are missing in the latter [6].
As already mentioned, the missing galactose can be
attributed to the phase variability of wlaN. The lack of
terminal GalNAc in this strain ... followed by a final annealing at 72 °C for 10 min using
primers: Cj1427cF918 (5¢-AACTTTCATCATTTTAAAC
GCTCTT-3¢) and Cj1427cR10 (5¢-AAAGTTTTAATTAC
AGGTGGTGCAG-3¢).
Deoxy...
... WSM.
Glycosaminoglycan analysis
Glycosaminoglycans are highly negatively charged because
of the presence of sulfate ester and/or carboxyl groups.
Therefore, they interact with cations and are precipitated ... hydrochloric acid [61]. The characterization of the
proteins associated to this complex by amino acid analysis
of the precipitate after two deacetylation steps revealed...
... In partic-
ular, the appearance of the ThDP adduct with the substrate
moiety during the complex-catalyzed succinyl-CoA hydro-
lysis [72] accounts for the inactivation by dihydrolipoamide
in the ... efficiently interact
with the E1 catalytic intermediate, as the lipoyl-bearing
domain of E2 is designed for this interaction (reaction 2). In
this case, 1e
–
oxidation...