Tài liệu Báo cáo Y học: Analyses of the CYP11B gene family in the guinea pig suggest the existence of a primordial CYP11B gene with aldosterone synthase activity docx

Tài liệu Báo cáo Y học: Analyses of the CYP11B gene family in the guinea pig suggest the existence of a primordial CYP11B gene with aldosterone synthase activity docx

Tài liệu Báo cáo Y học: Analyses of the CYP11B gene family in the guinea pig suggest the existence of a primordial CYP11B gene with aldosterone synthase activity docx

... decrease in the activity for the 11b-hydroxylase, CYP11B1 . Intriguingly, however, the 11b-hydroxylase activity of the aldosterone synthase CYP11B2 was basically unaffected whereas the 18-hydroxy- lation ... to adrenodoxin when compared with CYP11B2 . CYP11B1 has a tryptophan at position 366 while all other proteins of the CYP11B family including CYP11B...
Ngày tải lên : 22/02/2014, 07:20
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Tài liệu Báo cáo Y học: Systematic search for zinc-binding proteins in Escherichia coli potx

Tài liệu Báo cáo Y học: Systematic search for zinc-binding proteins in Escherichia coli potx

... structural factor for folding of domains involved in protein–protein and protein–DNA interactions. A large majority of the zinc-containing enzymes have a single zinc site consisting of a combination ... Department of Physics, Takatsuki, Osaka, Japan AsystematicsearchforEscherichia coli proteins with the zinc-binding activity was performed using the assay of radioac...
Ngày tải lên : 22/02/2014, 04:20
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Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf

Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf

... Chile; 2 Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Canada Escherichia coli phosphoenolpyruvate (PEP) carboxykinase catalyzes the decarboxylation of oxaloacetate and ... catalyzes the reversible decarb- oxylation of oxaloacetic acid (OAA) with the associated transfer of the c-phosphoryl group of ATP to yield PEP and ADP, where M 2+...
Ngày tải lên : 21/02/2014, 01:21
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Tài liệu Báo cáo Y học: The glucose-specific carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx

Tài liệu Báo cáo Y học: The glucose-specific carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx

... mutually interacting binding sites containing an active-site cysteine that is accessible from both faces of the membrane. The nature of the structural rearrangement for this alternating accessibility ... the other hand, the rate of inactivation by bromoacetyl-Glc ( 1a) was 15 times faster than by bromoacetic acid and 2.5 times faster than by the chemically more reactive iodo...
Ngày tải lên : 21/02/2014, 01:21
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Tài liệu Báo cáo Y học: Dnmt3a and Dnmt1 functionally cooperate during de novo methylation of DNA pdf

Tài liệu Báo cáo Y học: Dnmt3a and Dnmt1 functionally cooperate during de novo methylation of DNA pdf

... Dnmt1 is controlled by interaction of the catalytic domain with the N-terminal part of the enzyme leading to an allosteric activation of the enzyme after binding to methylated DNA. J. Mol. Biol. ... additional DNA binding site in its N-terminal part [33]. The observation that Dnmt 3a is not activated by pre-exiting methylation agrees with the fact that the rates of...
Ngày tải lên : 21/02/2014, 01:21
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf

Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf

... detected against any of the transgalactooligosaccharides listed in Materials and methods. Hydrolysis of arabinogalactans The sugar composition of the arabinogalactans from potato, onion and soy was ... galacto-oligosaccharides are detected. These disappear in time, suggesting the presence of an arabinose-releasing activity in the GALA preparation. However, no a- 1-,...
Ngày tải lên : 21/02/2014, 01:21
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Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf

Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf

... activity as described above, SOD concentrations having been calculated to yield 1 U SOD activity in a standard 1 mL assay. Activities were expressed as a percentage of SOD activity at 25 °C without ... program CE [35] while mutational analyses were carried out using the CARA and ENCAD algorithms included in the GENEMINE program. Bacterial strains and vectors The mutage...
Ngày tải lên : 21/02/2014, 01:21
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Tài liệu Báo cáo Y học: The structures of the lipooligosaccharide and capsule polysaccharide of Campylobacter jejuni genome sequenced strain NCTC 11168 pdf

Tài liệu Báo cáo Y học: The structures of the lipooligosaccharide and capsule polysaccharide of Campylobacter jejuni genome sequenced strain NCTC 11168 pdf

... the terminal galactose and GalNAc are missing in the latter [6]. As already mentioned, the missing galactose can be attributed to the phase variability of wlaN. The lack of terminal GalNAc in this strain ... followed by a final annealing at 72 °C for 10 min using primers: Cj1427cF918 (5¢-AACTTTCATCATTTTAAAC GCTCTT-3¢) and Cj1427cR10 (5¢-AAAGTTTTAATTAC AGGTGGTGCAG-3¢). Deoxy...
Ngày tải lên : 21/02/2014, 01:21
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Tài liệu Báo cáo Y học: Soluble silk-like organic matrix in the nacreous layer of the bivalve Pinctada maxima A new insight in the biomineralization field pptx

Tài liệu Báo cáo Y học: Soluble silk-like organic matrix in the nacreous layer of the bivalve Pinctada maxima A new insight in the biomineralization field pptx

... WSM. Glycosaminoglycan analysis Glycosaminoglycans are highly negatively charged because of the presence of sulfate ester and/or carboxyl groups. Therefore, they interact with cations and are precipitated ... hydrochloric acid [61]. The characterization of the proteins associated to this complex by amino acid analysis of the precipitate after two deacetylation steps revealed...
Ngày tải lên : 21/02/2014, 01:21
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Tài liệu Báo cáo Y học: Inactivation of the 2-oxo acid dehydrogenase complexes upon generation of intrinsic radical species pptx

Tài liệu Báo cáo Y học: Inactivation of the 2-oxo acid dehydrogenase complexes upon generation of intrinsic radical species pptx

... In partic- ular, the appearance of the ThDP adduct with the substrate moiety during the complex-catalyzed succinyl-CoA hydro- lysis [72] accounts for the inactivation by dihydrolipoamide in the ... efficiently interact with the E1 catalytic intermediate, as the lipoyl-bearing domain of E2 is designed for this interaction (reaction 2). In this case, 1e – oxidation...
Ngày tải lên : 21/02/2014, 01:21
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