Tài liệu Báo cáo Y học: Induction of (2¢)5¢)oligoadenylate synthetase in the marine sponges Suberites domuncula and Geodia cydonium by the bacterial endotoxin lipopolysaccharide docx
... Biochem. 269) Ó FEBS 2002
Induction of (2¢)5¢)oligoadenylate synthetase in the marine
sponges
Suberites domuncula
and
Geodia cydonium
by the bacterial endotoxin lipopolysaccharide
Vladislav A. ... to
24 h . The i dentity of the p
3
A
2
product synthesized by the
(2–5)A synthetase both in tissue and in primmorphs of
S. domuncula was v...
... is involved in many protein
folding and traffic processes i n the cell. The main compo-
nent of the system is DnaK, a t wo-domain protein with a
C-terminal domain responsible for the binding of ... unfolded
hydrophobic peptides and a N-terminal domain, which
binds ATP. This protein can bind and release peptides (in
the Ct domain) in a cycle driven by nucleotide hydro...
... identified,
including CYP1A1, human keratin 4, intestinal alkaline
phosphatase, ornithine decarboxylase, histidine decar-
boxylase and cyclin D1 [17]. KLF4 regulates the target
genes by binding to the ... reporter assay. The results showed that
induction of KLF4 by high-density lipoproteins could promote the expres-
sion of SR-BI, resulting from the binding to putative KL...
... progression, the understanding of these underly-
ing intracellular events is of importance. In the present
study, we explored the signaling pathway linking disrup-
tion of E-cadherin-dependent ... component of
the cell–cell adhesion complex and required for the for-
mation of epithelia in the embryo and the maintenance
of the polarized epithelial structure...
... between FeSOD and
MnSOD [7]. The position of the glutamine residue, which is
structurally equivalent in the enzymes, is contributed by the
N domain of FeSODs (Q69) and the C domain of MnSODs
(Q146). ... wild-type enzymes, the
metal sites of the purified proteins are either completely
(FeSOD, 1.17) or nearly fully occupied by the metal ion
from which the SOD...
... appearance of the ThDP adduct with the substrate
moiety during the complex-catalyzed succinyl-CoA hydro-
lysis [72] accounts for the inactivation by dihydrolipoamide
in the presence of succinyl-CoA ... network of interacting lipoyl moieties of the E2 core
provides a good explanation for these phenomena.
Considering possible mechanisms of the inactivation of
the...
... performed by
using different input order of the sequences. Each analysis
involved two steps: stepwise addition and nearest neighbor
interchanges. The most likely tree was obtained by using the
test of ... hydrophobic
ligands, binding to bilirubin and carcinogens [3,4], the
isomerization of maleylacetoacetate and the regulation of
stress kinases and apoptosis [5,6...
... bis-hydroxymethylated. The purity
of these compounds (> 96%) was checked by
1
HNMR
and HPLC.
Spectrophotometric determination of laccase activity
The activity of laccase was determined by following the rate
of ... N-hydroxyphtalimide (HPI) and 2,2,6,6-
tetramethylpiperidin-1-yloxy free radical (TEMPO) as
mediators, since they greatly differ both in their mechan-
ism...
... in
psychrophilic enzymes. Glycine residues are highly con-
served in the sequences of the proteinases compared in
this study. For example, of the 37 glycines present in the
proteinase domain of ... domain (see Fig. 2 or 3). The proteinase domain
of VPR additionally contains Cys277 and Cys281, and
Cys351 and Cys362 in the C-terminal domain, that suppo-
sedly...
... maintenance of tumor cell growth
[3]. The finding that the ERK signaling pathway is involved
in tumor cell migration and invasion [4,5], as well as in
tubular formation induced by insulin in human endothelial
cells ... NAMI-A-mediated inhibition
of ERK1/2 phosphorylation may be lying upstream of
ERK1/2.
Using a serine/threonine kinase SPA assay kit, the
capability of NA...