Tài liệu Báo cáo Y học: Study of substrate speciﬁcity of hum

Tài liệu Báo cáo khoa học: Molecular and cellular speciﬁcity of post-translational aminoacyl isomerization in the crustacean hyperglycaemic hormone family docx

... Peptide Synthesizer (Applied Biosystems, Foster City, CA, USA) as described previously [51]. The homogeneity of the synthetic peptides preparation was assessed by MALDI-TOF MS and analytical RP-HPLC. Decapeptides ... 481–485. 10 Ollivaux C & Soyez D (2000) Dynamics of biosynthesis and release of crustacean hyperglycemic hormone isoforms in the X-organ-sinus gland complex of th...

Ngày tải lên : 18/02/2014, 11:20

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Tài liệu Báo cáo Y học: Study of substrate speciﬁcity of human aromatase by site directed mutagenesis pdf

... relationships of human aromatase (Eur. J. Biochem. 269) 1405 Study of substrate speciﬁcity of human aromatase by site directed mutagenesis P. Auvray 1, * ,† , C. Nativelle 1,† , R. Bureau 2 , P. Dallemagne 2 , ... mechanism of aromatase cytochrome P450. A site- directed mutagenesis study. Eur. J. Biochem. 268, 243–251. 63. Gaillard, J.L. (1991) Equine tes...

Ngày tải lên : 21/02/2014, 15:20

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Tài liệu Báo cáo Y học: The RuvABC resolvasome Quantitative analysis of RuvA and RuvC assembly on junction DNA doc

... Rafferty 3 , Robert G. Lloyd 2 , Jane A. Grasby 4 and David P. Hornby 1 1 Transgenomic Research Laboratory, Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheﬃeld, UK; 2 Institute ... over only a 10-fold increase in protein concentration, nearly 100% of the binding sites are occupied by RuvA. The concentration at which approximately 50% of the DNA...

Ngày tải lên : 21/02/2014, 01:21

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Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx

... the cystatin F produced by U937 cells is retained intracellularly or is taken up by the cells immedi- ately following secretion. Accordingly, the relatively low amount of cystatin F produced by ... to detect cystatin F directly in culture media or cell lysates by immunoblotting. How- ever, by absorption on Sepharose-bound Cm-papain fol- lowed by Western blot analysis, the cell l...

Ngày tải lên : 21/02/2014, 01:21

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Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt

... DISCUSSION By site- directed mutagenesis, a D153N mutant form of E. coli agmatinase was obtained. The mutant enzyme retained about 5% of wild-type activity and it was equally active when assayed in ... (Asp153), may be reason- ably expected. This expectation is supported by our present ﬁndings of a markedly decreased activity of a D153N variant of E. coli agmatinase. From th...

Ngày tải lên : 21/02/2014, 01:21

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Tài liệu Báo cáo Y học: Intracellular localization and transcriptional regulation of tumor necrosis factor (TNF) receptor-associated factor 4 (TRAF4) pdf

... Wajant 1 1 Institute of Cell Biology and Immunology and 2 Institute of Industrial Genetics, University of Stuttgart, Germany; 3 Institute of Immunology, Christian-Albrechts-University of Kiel, Germany To gain ... 8). Correspondingly, ﬂuorescence of cytoplasmic T4(259–470)– YFP and full-length T4–GFP was already signiﬁcantly reduced after 2 min of bleaching in a small area of...

Ngày tải lên : 21/02/2014, 15:20

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Tài liệu Báo cáo Y học: Studies on the nonmevalonate pathway of terpene biosynthesis The role of 2C-methyl-D -erythritol 2,4-cyclodiphosphate in plants docx

... a cyclic diphosphate by the consecutive action of 4-diphosphocytidyl-2C-methyl- D- erythritol synthase, 4-diphosphocytidyl-2C-methyl- D-erythritol kinase and 2C-methyl- D-erythritol 2,4-cyclodiphosphate synthase ... that [1- 3 H]2C-methyl-D-erythritol 4-phosphate was diverted efﬁci- ently to the 2C-methyl- D-erythritol 2,4-cyclodiphosphate Fig. 3. 13 C NMR signals of 2C-methyl-D-erythri...

Ngày tải lên : 22/02/2014, 07:20

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Tài liệu Báo cáo Y học: The solution structure and activation of visual arrestin studied by small-angle X-ray scattering pot

... phosphorylation of the C-terminus of R* and binding by arrestin. Phosphorylation somewhat decreases the ability of R* to signal transducin. Rapid shut- off of R* signalling is then accomplished by ... membrane hyper- polarization and the sensation of light (reviewed in [1]). The sensitivity of the system requires that the signal transmitted by R* be rapidly attenuated. This...

Ngày tải lên : 22/02/2014, 07:20

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Tài liệu Báo cáo khoa học: Structural and functional speciﬁcities of PDGF-C and PDGF-D, the novel members of the platelet-derived growth factors family docx

... expression of highly speci- fic proteases that proteolytically activate the PDGFs will also influence the availability and activity of the dif- ferent isoforms. This can be exemplified by the proteo- lytic ... interstitial cells in a diversity of renal diseases but not in normal human kidneys. Most interestingly, PDGFR-a is extensively expressed by interstital cells of normal kidne...

Ngày tải lên : 19/02/2014, 07:20

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Tài liệu Báo cáo khoa học: Changes in purine speciﬁcity in tandem GAF chimeras from cyanobacterial cyaB1 adenylate cyclase and rat phosphodiesterase 2 pptx

... with replacement of this segment by the rPDE2 region, which comprised only 23% of the residues of the cyaB1 GAF B domain, was exclusively, yet weakly, sti- mulated by cGMP, whereas the activation by cAMP was ... lm, similar to wild-type cyaB1, and, surpris- ingly, activation by cAMP was eliminated (Fig. 2A, Table 1). Obviously, exchange of this region switched the speciﬁcity...

Ngày tải lên : 19/02/2014, 02:20

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