Tài liệu Báo cáo Y học: Proteolysis of bovine b-lactoglobulin during thermal treatment in subdenaturing conditions highlights some structural features of the temperature-modified protein and yields fragments with low immunoreactivity pptx
... 2002
Proteolysis of bovine b-lactoglobulin during thermal treatment
in subdenaturing conditions highlights some structural features
of the temperature-modified protein and yields fragments
with low ... B.W. (1993) Enzymatic hydro-
lysis of whey proteins. In uence of heat treatment of alpha-lac-
talbumin and beta-lactoglobulin on their p...
... protease
cathepsin D, as indicated by the following observa-
tions: (a) the inhibitor profile of the endosomal
ETA-degrading activity was very similar to that of
cathepsins B and D [30]; and (b) the ... determined. Finally, we assign
an important role to the endosomal acidic cathep-
sins B and D in increasing the in vitro transfer of
the ADP-ribosyl moiety of NA...
... visualized by SOD activity staining may have
been derived from further interactions of the fusion protein
GST domains (Fig. 2B). Although not visualized in these
zymograms, all of the fusion proteins ... combination of key residues.
Further analysis of these and other mutant SODs is
currently underway.
ACKNOWLEDGEMENTS
We are indebted to G. Peplow, F. Yamakura and T. Matsu...
... appearance of the ThDP adduct with the substrate
moiety during the complex-catalyzed succinyl-CoA hydro-
lysis [72] accounts for the inactivation by dihydrolipoamide
in the presence of succinyl-CoA ... several lines of evidence
point to the presence of the thiyl radical in the aerobic
system. First, the complexes are inactivated by 2-oxo acid
and CoA in...
... model the C-terminal domain of SmGSTO
due to the low rate of identity between the two proteins in
this region. However, as the active sites of the GSTO1-1 are
located at the N-terminal domain, ... are in line with the high GSH K
m
value obtained for
SmGSTO and the strong inhibitory effect of S-hexyl
glutathione on the enzyme activity. The preference of...
... consump-
tion. On the other hand, the laccase/TEMPO system
performs the selective oxidation of the hydroxymethyl
groups of the substrate to aldehydes. Both with laccase
alone and with the laccase/HPI system, ... promotes the formation of the same phenoxy radical
intermediate as laccase, yields the same products as the
enzyme alone, namely, ring-opening and...
... N-terminal of the
proteinase domain (see Fig. 2 or 3). The proteinase domain
of VPR additionally contains Cys277 and Cys281, and
Cys351 and Cys362 in the C-terminal domain, that suppo-
sedly also ... high
homology to proteinases of the proteinase K family of
subtilisin-like proteinases. With respect to the enzyme
characteristics compared in this study the prope...
... NAMI-A-mediated inhibition
of ERK1/2 phosphorylation may be lying upstream of
ERK1/2.
Using a serine/threonine kinase SPA assay kit, the
capability of NAMI-A to affect the ERK1/2 phosphoryla-
tion activity ... as an
internal control for mRNA integrity and equal loading. The
levels of radioactivity incorporated into c-myc product were
normalized by comparison with the level...
... mutated
negatively and positively charged residues on the proposed
interaction site of plastocyanin with cytochrome f and
analysed the reaction of these mutants with the soluble
redox-active domain of cytochrome ... Further-
more, the type I copper protein plastocyanin can be
replaced by cytochrome c
6
, a redox protein of similar size
but entirely different foldin...
... aminoglycoside,
kanamycin, the a minocyclitol glycoside, streptomycin, t he
penicillins, ampicillin and penicillin G, the aminocyclitol,
spectinomycin, tetracycline and Cl-tetracycline, and the
macrolide, erythromycin) ... ata
con®rmed the possible binding of Cl-tetracycline a nd
streptomycin to the pre-mRNA. With Cl-tetracycline
and s treptomycin, the preincubation re...