... teaching was not often a very elevating process; and too often
appealed to the motive of fear, either of a black dog in the cellar or of the assurance that Buonaparte was
coming! Education of the ... pound of butter, cheese, and so on through all the products of the land. The
inconvenience of this clumsy system was often greatly felt, when a farmer was compelled to del...
... of ETA, or whether it involves the dual het-
erogeneous part of the toxin. Use of this approach will
provide novel insight(s) into the physiological signifi-
cance of the endosomal fragments of ... vitro proteolysis of ETA by hepatic endosomes
and proteases
The endosomal fraction ( 1 lg) was incubated for varying
lengths of time at 37 °C with 10 lg of native ETA in 30 l L...
... exon 7 of fragment C represents a dele-
tion of 39 bp. The thick lines indicate intron 6 of 75 bp in fragments
B and C, 3¢ portion of 35 bp in intron 5 in fragments C and D, and
3¢ portion of 25 ... activity of the )1386M construct was at least
eightfold higher than that of pGL3–basic vector and
was half that of pGL3–promoter vector. These findings
demonstrate the promo...
... 10.0;
GAPEXT
,0.05;
GAPDIST
,8;
MAXDIV
,
40;
ENDGAPS
,off;
NOPGAPS
,off;
NOHGAPS
,off.Graphical
output of the bootstrap figure was produced by the program
TREEVIEW
.
Isolation of RNA and RT-PCR analysis
Total RNA was isolated from ... fish
species. Here, we present evidence for existence of two
distinct HSF1 isoforms in rainbow trout and the formation
of heterotrimers of these i...
... using NADH was not affected. Like the
wild type, the mutant showed allosteric behaviour toward
BPGA and its K
0.5
was twofold higher, whatever the
cofactor. The kinetic parameters of the purified ... 1 lgofGAPDH($ 0.08 nmol)
and 0.08 lgofCP12($ 0.08 nmol) were mixed and 1 lgofthemix-
ture was analyzed. In lane 2, 38 lg o f the K 128A mutan t and 3 lgof
CP12 were mixed and about 1 0 lg...
... 4673–4680.
J. To
¨
zse
´
r et al. Comparison of two potyvirus proteases
FEBS Journal 272 (2005) 514–523 ª 2004 FEBS 523
Comparison of the substrate specificity of two potyvirus
proteases
Jo
´
zsef To
¨
zse
´
r
1
, ... key
determinants of substrate specificity. The specificity of the mutant enzymes
was assessed by using peptides with complementary substitutions. The crys-
tal stru...
... rate of accumulation of the
two transcripts under these conditions. Induction for longer
periods of time did not further change the levels of the two
transcripts (not shown). The induced rates of ... the turnover
of the D1 protein of photosystem II (PSII), causes the
partial inhibition of PSII, and leads to a reduction of
photosynthetic O
2
evolution. The partial inhibit...
... Antiviral Program of the Office of the Director of NIH and
by AIDS FIRCA Grant TW01001. The contents of this publication
do not necessarily reflect the views or policies of the Department of
Health and ... zse
´
r
1
1
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, Hungary;
2
Department of
Biology, Georgia State University, Atlan...
... leftovers of strand D via a disulfide b ridge to
Cys66 [5].
Immunoreactivity of the products of BLG hydrolysis
As stated in the introduction, one of the goals of this work
was to t ake advantage of the ... smaller peptides) when enough
enzyme activity was present (that is, at relatively low
Fig. 4. Time course of the formation of fragments having different size
during pr...
...
There are two benches to the left of the
shop
*There are two benches to the left of the shop.
Hình thức ngữ pháp : cấu trúc: “there are/is….to the left of ” – (có cái gì ở bên ... từ đó)
There are two benches to the left of the shop.
2. Các bạn hãy di chuột vào từng cụm từ một để biết chức năng của cụm trong
câu:
There are two benches to the left of the sh...