... Characterization of phycoviolobilin phycoerythrocyanin-a84-cystein-
lyase-(isomerizing) from
Mastigocladus laminosus
Kai-Hong Zhao
1
, Dong Wu
1
, Lu ... His
6
-PecF). Addition of Triton X-100 to the reaction
mixture resulted in an absorption shift of the long
wavelength band of PCB from 620 nm to 600 nm
(Fig. 2), irrespective of the presence of the lyase ... 100
Fig....
... 10
6
mL
)1
. Next, 0.5 mL of
propidium iodide staining solution (10 mg of propidium
iodide, 0.1 mL of Triton-X, 3.7 mg of EDTA in 100 mL of
NaCl ⁄ P
i
) was added to 0.5 mL of cell suspension. The ... spectra of extracted lipids (A) and PCA extracts (A¢) from one representative sample of MCF-7 cells; 2D
1
H NMR spectra
of extracted lipids (B) and PCA extracts (B¢) from o...
... gigas.
In this article, we report the characterization of a
new CLP, named Cg-Clp2, from the oyster C. gigas.
The tissue distribution and temporal pattern of expres-
sion of the gene encoding Cg-Clp2 ... conserved amino acid sequences of the
catalytic domain of members of the GH18 family
resulted in the amplification of an expected 147 bp
sequence. Cloning and sequencing of...
... number of transcripts containing exon 1a
with the number starting from exon 1. The abun-
dance of transcripts starting from exon 1 was 10-fold
higher than that of the transcripts starting from
exon ... 5¢-flanking
region of exon 1 in DNASE1. Deletion of the
upstream end of the 5¢-flanking region of exon 1 from
position )1386 to )231, )116, or )78 did not result in
any sign...
... are from one experiment, representative of
three giving similar results.
Fig. 6. Dependence on the concentration of PPI1
588
His
6
of the sti-
mulation of H
+
-ATPase activity in PM purified from ... the mechanism of action of PPI1.
The analysis of the pH dependence of PPI1-
induced activation of the H
+
-ATPase showed that
stimulation decreases dramatically with the incr...
... Proteinase K from
Tritirachium album Limber. Characterization of the
chromosomal gene and expression of the cDNA in
Escherichia coli. Eur J Biochem 179, 185–194.
Characterization of a Serratia ... Isolation of genomic DNA from Serratia sp.
Genomic DNA was isolated as described by Chen and Kuo
[38] for use in identification of the peptidase gene.
Generation of an 200-bp fr...
... of all of these genes remains to be clarified.
Expression and purification of Sno1p and Snz1p
In light of the similarity in the amino acid sequence of
Sno1p to that of the glutaminase subunit of ... absorbance of
the supernatant was read at 363 nm. The absorbance of
the reduced form of APAD (APADH) was linear over the
2–100 l
M
range of glutamate with a molar extinction
co...
... differences
between the TP isoforms in terms of their mechanisms
of signaling [18,19], modes of regulation⁄ desensitiza-
tion [20–23] and patterns of expression [24], this repre-
sents a question of potentially ... (2002) Characteri-
zation of the 5¢ untranslated region of alpha and beta
isoforms of the human thromboxane A2 receptor (TP).
Differential promoter utilization by t...
... data.
Data analyses
Data were analysed by means of sigmaplot 2001 (SPSS
Science Software Gmbh, Erkrath, Germany). Data for the
characterization of the kinetics of electrogenic BSP uptake
fitted the Michaelis–Menten ... – University of Trieste) for the immunization
and bleeding of rabbits; to Silvia Zezlina for the affin-
ity purification of antibody A from rabbit sera; to Dr
Paol...
... the number of attached cells in
four randomly chosen fields from duplicate wells. The
data was presented as a percentage of bound cells
(amount of bound red cells divided by input of cells). For
blocking ... percentage of attached cells
(amount of bound cells divided by input of cells). The amounts of bound and added red cells were quantitated by counting cells in four random...