... solve the solution structure
of the reduced dimeric protein (by using a classical NMR
approach) in order to compare the solution structures of the
monomeric and dimeric species as well as the solution ... dimeric Cu,Zn SOD (Eur. J. Biochem. 269) 1915
The solution structure of reduced dimeric copper zinc superoxide
dismutase
The structural ef...
... present for the last four residues at
the C-terminus. The stereochemistry of the model has
been assessed using the program procheck [23]. Ninety
per cent of the residues fall in the most favoured
regions ... mol-
ecules. When the R-factor decreased to a value of 0.27
at 2.0 A
˚
resolution, inspection of the electron density
map in the enzyme active site clearly rev...
... A
˚
resolution by the molecular replacement method. The
schematic view of the overall structure is shown in Fig. 3A.
Theenzymeexistsasadimeroftheabc heterotrimer. There
is a noncrystallographic twofold ... twofold axis around the center of
Fig. 3A. The structure of an abc heterotrimer unit is shown
in Fig. 3B. The central region of the a subunit constitutes
the (b/...
... turns
of the DNA double helix yields binding of the two or
three NF-Y complexes on the same side of the DNA.
Conservation of spacing is required for optimal pro-
moter activity because changing the ... However, as in most other CDE ⁄ CHR
promoters, mutation of the CHR results in a smaller
remaining cell cycle-regulation than alteration of the
CDE [37]. Interestingly,...
... involved either in the assembly of the immature
HIV-1 capsid, or in the reassembly of CA into a
mature capsid. The detailed description of the structure
and conformational dynamics of these interfaces,
together ... helix 4 of
the NTD of a neighboring subunit in the hexamer.
Additional contacts involve helix 11 of the CTD and
the C-terminal end of helix 7 o...
... at Thr188. The two addi-
tional aromatic residues in each monomer, Tyr164 and
Fig. 1. Structure of the CTD dimer. X-ray structure of CTD showing
the dimeric structure of the domain. The monomers ... the X-ray structure of the nonmu-
tated-CTD–CAI complex [28] (Fig. 2A, main panel).
The aromatic moiety of Phe3, together with Leu6 and
Ile1, are the residues...
... Furthermore, the pro-peptide
of GDF-8 impairs interaction of the mature part with
its receptors [16]. In the case of BMP-9, the pro-pep-
tide appears not to alter significantly the activity of the
mature ... here suggest that the
pro-domain of BMP-2 can alter the signalling properties of the growth factor
by modulating the ability of the mature part to inter...
... into the chitinolytic poten-
tial of L. lactis, the present results provide only the
third example of the role of family 33 CBPs in the deg-
radation of recalcitrant polysaccharides. Furthermore,
the ... [10].
Although the occurrence of family 33 CBPs has been
known for some time [23], the present results provide
only the third demonstration of the accessory fu...
... e.g.
the case of the association between the N-terminal
region of the membrane-bound tyrosine kinase Lck
with the cytoplasmic tail of the T-cell coreceptors CD4
or CD8 [37].
In solution, on the ... dispersion
of 0.16 p.p.m. (supplementary Figure S1). The lack of
chemical shift dispersion in the HN region as well as
in the methyl region (data not shown) is an ind...
... (enzyme
I of the PTS), the first protein in the cascade of proteins
forming the PTS, to HPr, the histidine-phosphocarrier
protein. HPr is the smallest protein in the protein cascade
of the PTS and ... from the fitting of the CD thermal denaturation data to Eqn (1). Fitting of the thermal and urea-denaturation data (using the
approach of Pace and Laurents [39]...