Tài liệu Báo cáo Y học: Characterization of a cloned subtili

Tài liệu Báo cáo Y học: Characterization of an omega-class glutathione S-transferase from Schistosoma mansoni with glutaredoxin-like dehydroascorbate reductase and thiol transferase activities pptx

... (GeneBank accession num- ber AI975843). A 549-bp fragment was ampliﬁed by PCR using oligonucleotides GSTX1: 5¢- GTTGTCGACAAA CATCTCAACTAG-3 and GSTX3: 5¢-GTAAGTGTGG GAATAAGATCAAATC from adult S. mansoni ... (parasitic stage of the intermediate host Biomphalaria glabrata) and adult worms (parasitic stage of human) than in others stages. Expression of SmGSTO was also studied at the...

Ngày tải lên : 21/02/2014, 01:21

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Tài liệu Báo cáo Y học: Characterization of a cloned subtilisin-like serine proteinase from a psychrotrophic Vibrio species doc

... homologues, aqualysin I from Thermus aquaticus and a proteinase from Thermus strain Rt4 1A, the cold-adapted Vibrio -proteinase has a higher content of polar/uncharged amino acids, as well as aspartate ... 5¢-ATGTTAAA GAAAGTATTAAGTTGTTG-TATTGCAGC-3¢ and 5¢-AAAGTTTGCTTGGAGCGTCAAGCC-ACTGTAAG CCG-3¢, cloned into the expression vector pBAD-TOPO, transformed into the E.coliTop10...

Ngày tải lên : 21/02/2014, 01:21

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Tài liệu Báo cáo Y học: Characterization of a novel silkworm (Bombyx mori ) phenol UDP-glucosyltransferase potx

... Technology and Medicine, London SW7 2AZ, UK; 2 Laboratory of Molecular Entomology and Baculovirology, Riken, Wako, Japan Sugar conjugation is a major pathway for the inactivation and excretion of both ... project. A wing disc cDNA library derived from ﬁfth instar B. mori C108 larvae was kindly provided by Dr Kawasaki (University of Utsunomiya, Japan). A total of 1000 clones we...

Ngày tải lên : 22/02/2014, 04:20

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Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf

... Biochemistry 39, 7131– 7137. 31. Hiraoka, B .Y. , Yamakura, F., Sugio, S. & Nakayama, K. (2000) A change of the metal-speciﬁc activity of a cambialistic superoxide dismutase from Porphyromonas gingivalis ... mutagenesis reactions together with oligonucleotides ECF-Q69G d(5¢-AACAACGCAGCT GGGCTCTG GAACCAT), ECF -A1 41Q d(5¢-TCAACCTCTAAC CAG GCTACTCCGCTG) ECM-G77Q d(5¢-AACAACGCTG...

Ngày tải lên : 21/02/2014, 01:21

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Tài liệu Báo cáo khoa học: Characterization of phycoviolobilin phycoerythrocyanin-a84-cysteinlyase-(isomerizing) from Mastigocladus laminosus pdf

... phycoerythrocyanin -a8 4-cystein-lyase(PVB- PEC-lyase) from Mastigocladus laminosus, which catalyses both the covalent attachment of phycocyanobilin to PecA, the apo -a- subunit of phycoerythrocyanin, and its isomeri- zation to phycoviolobilin. ... to ENZMES, as an enzyme of the subclass 4.4.1, as an alternative name we proposed holo -a- phycoerythrocyanin synthase, in analogy to the...

Ngày tải lên : 21/02/2014, 03:20

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Tài liệu Báo cáo Y học: Characterization and regulation of yeast Ca2+-dependent phosphatidylethanolamine-phospholipase D activity docx

... pattern raises the possibility that Ca 2+ may have a dual mechanism of action in activating PtdEtn-PLD, e.g. Ca 2+ may participate in catalysis as well as facilitate enzyme– substrate interaction. ... preferentially hydrolyses phosphatidylethanolamine (PtdEtn) and phosphatidylserine and does not catalyse a transphosphatidylation with primary short-chain alcohols. We have characterized...

Ngày tải lên : 22/02/2014, 07:20

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Tài liệu Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas doc

... cells from human adenocarcinomas Anna Maria Luciani 1 , Sveva Grande 1 , Alessandra Palma 1 , Antonella Rosi 1 , Claudio Giovannini 2 , Orazio Sapora 3 , Vincenza Viti 1 and Laura Guidoni 1 1 Dipartimento ... spectra. Time (day) Time (day) Time (day) Time (day) A/ (Lys + Ala) c A/ (Lys + Ala) i A B A B′ C Fig. 5. Intensity modulation of ML signals from a representative experimen...

Ngày tải lên : 18/02/2014, 13:20

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Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx

... sequencing chemistry. Real-time quantitative PCR Quantitative RT-PCR analysis was performed using the iCycler apparatus (Bio-Rad, Hercules, CA, USA). Total RNA was isolated from oocytes, embryos, larvae and adult tissues ... (5¢-GCATAGCGATGTGGACGA-3¢) and QaCgClp2 (5¢-GAGGACCGAGACCGTGAA-3¢). The abbreviations ‘Qs’ and ‘Qa’ refer, respectively, to sense and antisense primers. Accurate ampliﬁ...

Ngày tải lên : 19/02/2014, 00:20

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Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt

... Kominato Y, Yamamoto F & Takizawa H (2002) Characterization of the human ABO gene pro- moter in erythroid cell lineage. Vox Sang 82, 39–46. 34 Yasuda T, Awazu S, Sato W, Iida R, Tanaka Y & Kishi ... Kumamoto T, Mor- ikawa N, Masamura K, Tada H, Ito S, Hoshizaki H, Oshima S et al. (2004) Diagnostic use of serum deoxyribonuclease I activity as a novel early-phase marker in...

Ngày tải lên : 19/02/2014, 06:20

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Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

... Characterization of the interaction between the plasma membrane H + -ATPase of Arabidopsis thaliana and a novel interactor (PPI1) Corrado Viotti, Laura Luoni, Piero Morandini and Maria Ida ... primers: gatggatcccatATGGGTG TTGAAGTTGTA annealing around the start codon of the Ppi1 ORF and gactcgagATTAGTCGACTTCTTACGC annealing just before the putative transmembrane domain (capital lette...

Ngày tải lên : 19/02/2014, 07:20

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