Tài liệu Báo cáo khoa học: Fluorescence analysis of the Hans

Tài liệu Báo cáo khoa học: Fluorescence analysis of the Hansenula polymorpha peroxisomal targeting signal-1 receptor, Pex5p pdf

... Fluorescence analysis of the Hansenula polymorpha peroxisomal targeting signal-1 receptor, Pex5p Raina Boteva 1 , Anne Koek 2 , Nina V. Visser 2 , ... investigate the binding of the dansylated PTS1 peptide L2 to HpPex5p and correlate the ﬂuorescence data with structural data, a molecular model of the HpPex5p was built based on the known structure of the...

Ngày tải lên : 21/02/2014, 00:20

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Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll ﬂuorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc

... amplitudes and rate constants of the two other phases. The above should be gov- erned by the balance between the rate of reduction and oxidation of the acceptor side of PSII by the avail- able electron ... half-time of the J–I phase were both decreased with DCMU, demonstrating that the J–I rise is not directly related to the reduction of the PQ pool. A further...

Ngày tải lên : 19/02/2014, 05:20

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Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... with BMAL1 and the bin ding to the E-box. Substitution of Arg65 for Ala abolished the interaction of DEC1 with BMAL1 and the binding of DEC1 to the E-box. However, substitution of His57 for Ala ... increase in the mutant mice, the disruption of DEC1 might be compensated by DEC2. Further investigation is required to clarify the functions of DEC1 and DEC2. The supp...

Ngày tải lên : 19/02/2014, 16:20

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Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx

... mitochondrial proteins, on the other hand, are speciﬁcally linked to one of the major patho- genetic mechanisms of PD [5]. Worthy of note is the complete disappearance of the VDAC-2 upon dopamine ... quanti- ﬁed on the basis of their relative volume (spot volume nor- malized to the sum of the volumes of all the representative spots) and those that consistently...

Ngày tải lên : 15/02/2014, 01:20

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Tài liệu Báo cáo khoa học: Kinetic analysis of effector modulation of butyrylcholinesterase-catalysed hydrolysis of acetanilides and homologous esters pdf

... found between the effects of these compounds on the AAA and esterase activities of human BuChE. The concentrations of tyramine and serotonin that activate or inhibit the AAA activity of BuChE (and also ... the titrant. The active site concentration of wild-type BuChE was 0.16 ± 0.01 lm, and that of the D70G mutant was 0.196 ± 0.02 lm. Effects of ligands on steady-s...

Ngày tải lên : 18/02/2014, 17:20

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Tài liệu Báo cáo khoa học: Thermodynamic analysis of porphyrin binding to Momordica charantia (bitter gourd) lectin pptx

... investigated the interaction of several water-soluble porphyrins with MCL. The thermodynamic forces governing the interaction of some of the porphyrins have been delineated from an analysis of the temperature ... investigate the effect of porphyrin b inding on the protein conformation. For these spectra, a spectrum of the buffer containing the same concentratio...

Ngày tải lên : 19/02/2014, 16:20

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Tài liệu Báo cáo khoa học: Subproteomics analysis of Ca2+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle pdf

... ÔStains- AllÕ analysis of Fig. 3 and the 1D i mmunoblotting of Fig. 4. As the full-length Dp427 isoform of dystrophin does not enter the second dimension of conventional 2D gels, the expression level of ... In contrast, the exp ression o f major i on-regulatory muscle components, such as the Na + /K + ATPase, the SERCA1 isoform of the sarcoplasmic reticulum Ca 2+...

Ngày tải lên : 19/02/2014, 16:20

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Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf

... bond, linkage of the active site Ser of the protease to the carboxyl group of P 1 by an ester bond and insertion of the N-terminal part of the RCL as strand 4 in b-sheet A (s4A) of the serpin. ... cognate proteases is fuelled by the thermodynamic properties of the serpin. Accordingly, insertion of the RCL as s4A and the ensuing structural rearrangements of...

Ngày tải lên : 20/02/2014, 11:20

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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... [26]. These ﬁndings lead to the hypothesis that Fe-bound ApeSOD mimics the product-inhibited form and the shift of Tyr39 sup- presses the release of the peroxide product. This may be one of the ... inhibition of the binding of the superoxide substrate and the other to product inhibition by hydrogen peroxide. There are two mechanisms proposed for the reaction cy...

Ngày tải lên : 14/02/2014, 22:20

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Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt

... manganese in the transcriptional regulation of the nrd operon [35] may not be considered in the light of the results obtained in the present study. Rather, the involvement of manganese in the in vivo ... to the catalytic site of the R1E subunit. As reported in the Results, upon completion of substrate conversion, the radical is then rapidly passed back from...

Ngày tải lên : 15/02/2014, 01:20

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