... Membrane orientation of laminin binding protein
An extracellular matrix bridging molecule of
Leishmania donovani
Keya Bandyopadhyay*
,
†, Sudipan Karmakar*, Aruna Biswas and Pijush ... either anti-LBP IgG or anti -laminin IgG.
Proteolytic digestion of
L. donovani
membrane
L. donovani membrane preparations were made according
to the method described previ...
... the importance of anchoring the tail of the ecto-
domain for both IGF-II and Man-6-P ligand binding.
Receptor binding on resin resembles a patchwork
model, where a multivalent ligand can cross-bridge
between ... (1986) Analysis of serum insulin-like growth
factor binding proteins using western blotting: use of the
method for titration of the binding proteins and competi-
tiv...
... pool of translocator
proteins is released rapidly and concurrently, and
effectors are translocated in an ordered manner
[16,17]. Translocon proteins presumably travel through
the interior of the ... formation
The composition of the host cell membrane appears to
be a critical point for the invasion of bacteria, insertion
of translocators and functionality of the pore. Target
m...
... supernatant and the membrane frac-
tions was similar to those of GGN, GGD, GGS, and
AAG (Figs 2B and 4B). Interestingly, similar to the
case of GGN mutant, a small but significant amount
of proteins ... stroma and to the
membrane (Fig. 4A, lanes 21–24; Fig. 4B,C) in a way
similar to GGA mutant (Fig. 4A, lanes 9–12;
Fig. 4B,C). Import of GGE mutant was less efficient
than that of o...
... m
t1
ÞÁD ð9Þ
where lnk
f1
and lnk
f2
are folding constants associated with
first and second transitions, respectively, at any given dena-
turant concentration, and lnk
u1
and lnk
u2
are the unfolding
J. ... (1991) Denatured states of
proteins. Annu Rev Biochem 60, 795–825.
36 Matouschek A & Fersht AR (1993) Application of
physical organic chemistry to engineered mutants of
proteins:...
... 4407
direct substrate of the reductase and can be progres-
sively regenerated from the a anomer by spontaneous
anomerisation.
Kinetic analysis of the recombinant proteins
from C. glabrata and A. aeolicus ... RIB7
protein of Methanocaldococcus jannaschii (accession number Q58085); ARCFU, RIB7 protein of Archaeoglobus fulgidus DSM 4304 (acces-
sion number O28272); CANGL (CglRED), RI...
... mass lynx v.4.0 software package.
Acknowledgements
We thank NSERC of Canada for financial support
(M. J. Stillman) and Postgraduate Scholarship (K. E.
Rigby Duncan). We also thank Professor R. J. ... noncooperative metal binding to the
a domain of human metallothionein
Kelly E. Rigby Duncan and Martin J. Stillman
Department of Chemistry, The University of Western Ontario, London, ON,...
... buffer. Shown are stefin A
and stefin B at pH 5 and variant 2 of ste-
fin B and the P79S mutant of the variant at
both pH 7 and pH 5. Each panel is a plot of
heat change on ligand addition (kcalÆmole
)1
)
against ... the
ligand-macromolecule reaction occured. A binding isotherm
was fitted to the data, expressed in terms of the heat change
per mole of ligand against the ligand to macro...
... considering membrane components, the secretory
(Sec) translocon is known to play an important role as
a protein- conducting channel for membrane integra-
tion and translocation [33]. The majority of membrane
proteins ... mature protein.
Synthesis of membrane proteins for
minimal cells
The goal of the new and rapidly developing field of
synthetic biology is the development...
... relevant to the regulation of
AC proteins.
In view of the important and antagonistic effects of
ADF ⁄ cofilin and phosphate on actin dynamics, we
examined here the binding of cofilin to F-actin and ... quenchers.
These changes transform the nucleotide cleft of F-actin to G-actin-like.
Pi regulation of cofilin binding and the cofilin regulation of Pi binding to
F-actin can be i...