... Evidence for proteasome dysfunction in cytotoxicity mediated
by anti-Ras intracellular antibodies
Alessio Cardinale, Ilaria Filesi, Sonia Mattei and Silvia ... Italy
Anti-Ras intracellular antibodies inhibit cell proliferation
in vivo by sequestering the antigen and diverting it from its
physiological location [Lener, M., Horn, I. R., Cardinale, A.,
Messina, .....
... tetra-
thiolate sites, the choice for the third incoming metal
ion would be to form a bridging interaction in the
a domain or to form another independent tetrathiolate
site in the b domain. It is probable that ... to coordinate to
the two-domain ba-MT in a domain-specific manner,
with a preference for the a domain, has been construed
as being an indicator of cooperative metal binding...
... an
essential twin-arginine motif in the N-terminal domain
[9,10]. They then interact with a translocon in the
inner membrane that consists, minimally, of three sub-
units (TatABC) in Escherichia ... specificity determinant for protein
secretion via the twin-arginine translocation pathway.
J Biol Chem 275, 41350–41357.
16 Pop O, Martin U, Abel C & Muller JP (2002) The twin-
arginine...
... changes in the cytoskeleton that
may occur during the initial 6 h window of infection.
We infected RPE cells with K26GFP [27], and stained
cells for F-actin, using phalloidin at 30 min and 6 h
postinfection, ... FEBS 5281
damine-conjugated phalloidin (Invitrogen) was added for
F-actin staining at room temperature for 45 min. Finally, the
cells were washed three times with 1· NaCl ⁄ P...
... Diego, CA, USA) binding and
PI staining were performed according to [44]. Following
incubation with 1.0 lgÆmL
)1
annexin V and 4.0 lgÆmL
)1
PI
for 20 min, cells were analyzed by flow cytometry.
Preparation ... detected by Nile red stain-
ing as previously described [42]. Cell imaging for Nile red
staining was performed by confocal microscopy. Quantifica-
tion of lipid droplets was per...
... protein 1 ⁄ core pro-
tein 2) in a large core structure that also
includes the chaperone protein Bcs1p. This
core structure is then able to sequentially
bind the remaining bc
1
subunits in a ... [45] and the transformants
were then selected for leucine prototrophy. The double
deletion strains were constructed by crossing selected single
deletion strains. The resulting diploids were sp...
... European Bioinformatics
Institute’s server (http://www.ebi.ac.uk/) for clu-
stalw2 [53] as Phylip-tree type and neighbour-joining
clustering [26] using the alignment of complete
sequences, including ... cDNA involved in cystine,
dibasic, and neutral amino acid transport. J Clin Invest
91, 1959–1963.
26 Saitou N & Nei M (1987) The neighbor-joining
method: a new method for reconstruct...
... A
synergistic increase in neutralizing antibody titers by
Table 1. Vaccines against dengue fever by the Initiative for Vaccine Research, World Health Organization, updated February 2006.
Type of vaccine
Pharmaceutical
company ... also for the correct interaction
with the substrate. The binding site of the NS2B cofac-
tor could be examined for a view to inhibitor develop-
ment, and...
... N-ter-
minal or C-terminal histidine tags. For that purpose, a cod-
ing sequence for six histidines was introduced by PCR
either at the N-terminus, after the ATG, or at the C-termi-
nus before the ... protein had a similar activity in crude yeast
extract as the untagged protein, indicating that the tag
was not interfering with the protein activity. The
tagged protein was purified and t...
... outlined in Figure 1.
Each Arabic letter has several forms. The particular
form selected in any given instance is determined by
the preceding and following letters. In general, there-
fore, in ... input sentence remains intact,
in order and form, as it does in the present program.
The only changes which are made are in the form of
added elements making grammatical inform...